Niflumic acid,prototype of a multiaction antithrombotic agent

Zusammenfassung Niflumsäure, Prototyp eines mehrfach wirkenden antithrombotischen Medikamentes, induziert Fibrinolyse in vitro und in vivo, hemmt Erythrozyten-und Thrombozytenaggregation und induziert eine stärkere Fibrinolyse vorgebildeter Gerinnsel als Strepto-und Urokinase.

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This study was supported by grant-in-aid No. HL 9985 National Heart and Lung Institute USPHS, Bethesda, USA.     

Elevation of blood glucose and induction of hepatic enzymes by 2-deoxyglucose in mice with hypothalamic damage caused by gold thioglucose

Zusammenfassung 2-Deoxyglukose führt bei Mäusen zu Blutzuckersteigerung und in der Leber zu Aktivierung der Tryptophan-Pyrrolase und Tyrosine-Transaminase bei durch Goldthioglukose verursachten hypothalamischen Schäden.

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I thankHarold D. Snoddy andBetty J. Warren for expert technical assistance.     

Hypothalamic tyrosine hydroxylase activity,plasma gonadotropin and prolactin levels after aminooxyacetic acid in ovariectomized rats

Summary Plasma concentrations of gonadotropin, prolactin and hypothalamic tyrosine hydroxylase (TH) activity were measured in ovariectomized rats treated with aminooxyacetic acid (AOAA), a drug which elevates brain GABA levels. Hypothalamic TH activity was significantly increased with a significant decrease in prolactin (Prl) release. Plasma levels of gonadotropins were not modified by AOAA. These results support an inhibitory action of GABA on Prl release possibly mediated through hypothalamic dopamine.Supported by grants from Indian Council of Medical Research (ICMR), New Delhi. RIA kits for the estimation of LH, FSH and Prl were kindly supplied by Dr A.F. Parlow, NIAMDD-NIH, Bethesda, Maryland, USA. GNB is a UGC research fellow.     

Hypothalamic tyrosine hydroxylase activity, plasma gonadotropin and prolactin levels after aminooxyacetic acid in ovariectomized rats

Plasma concentrations of gonadotropin, prolactin and hypothalamic tyrosine hydroxylase (TH) activity were measured in ovariectomized rats treated with aminooxyacetic acid (AOAA), a drug which elevates brain GABA levels. Hypothalamic TH activity was significantly increased with a significant decrease in prolactin (Prl) release. Plasma levels of gonadotropins were not modified by AOAA. These results support an inhibitory action of GABA on Prl release possibly mediated through hypothalamic dopamine.     

Temperature compensation in an ultradian rhythm of tyrosine aminotransferase activity in Euglena gracilis Klebs

Tyrosine aminotransferase activity of Euglena oscillates with an ultradian period of approximately 4-5 h. The oscillation frequency in the time series was determined by cosine fitting. Experiments which were performed between 16 and 31.5 degrees C revealed temperature compensation.     

Formation of 2-hydroxy-6-oxo-2,trans-4,trans-heptadienoic acid from 3-methylcatechol by aPseudomonas

Riassunto Ps. desmolyticum ossida la 3-metilpirocatechina ad acido 2-ossi-6-oxo-2,trans-4,trans-eptadienoico. Vengono descritte le proprietà chimicofisiche che hanno condotto all'identificazione di questo intermedio metabolico.

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This work was supported by C.N.R. (Consiglio nazionale delle Ricerche).     

Inhibition of tyrosine degradation in vivo by the dopa decarboxylase blocking agent NSD-1034

Zusammenfassung Der Stoffwechsel von H ₃ ² -3, 5-l-Tyrosin wurde in mit NSD-1034 (100 mg/kg) vorbehandelten Versuchstieren (Hähnchen, katzen) untersucht. Als Folge des durch NSD-1034 stark gehemmten Abbaus entstanden während der Versuchszeit (ca. 20 min) nahezu kein Tritiumwasser und keine aromatischen Abbauprodukte. Auch die sonst zu beobachtenden radioaktiven Metabolite des H³-Tyrosins (Glutamin- und Asparaginsäure, Dopamin und Noradrenalin) waren nicht nachweisbar. Ausserdem war der Gehalt an H³-Tyrosin in Organen behandelter Tiere höher als in Kontrollen.     

4-methylcatechol,a metabolite of homoprotocatechuic acid

Zusammenfassung Homoprotocatechusäure wird in der Ratte bei oraler Zufuhr teilweise durch Dekarboxylierung zu 4-Methylcatechol abgebaut, eine Reaktion, die von oxytetracyclinsensitiven Darmbakterien ausgeführt werden kann.     

Influence of flupirtine,a novel nonopioid analgesic agent on somatosensory evoked potentials in rats

The effect of flupirtine, a novel nonopioid analgesic, on somatosensory evoked potentials (SEP) was investigated in anesthetized rats. Primary somatosensory potentials were evoked in the cerebral cortex by stimulation of the skin of the whiskery part of the face. Flupirtine injected i.p. dose-dependently prolonged the latency and reduced the amplitude of SEP with ID₅₀-values of 5.4 mg/kg (2.6–9.3 mg/kg) and 7.9 mg/kg (3.9–13.8 mg/kg), respectively. This effect of flupirtine (10 mg/kg, i.p.) on the latency and the amplitude of SEP, did not change when naloxone (1 mg/kg, i.p.) was given before flupirtine. The results indicate that the analgesic flupirtine decreases the primary somatosensory evoked potential by diminishing the excitability of cortical neurons. Opioid mechanisms are not involved.     

Influence of flupirtine, a novel nonopioid analgesic agent on somatosensory evoked potentials in rats.

The effect of flupirtine, a novel nonopioid analgesic, on somatosensory evoked potentials (SEP) was investigated in anesthetized rats. Primary somatosensory potentials were evoked in the cerebral cortex by stimulation of the skin of the whiskery part of the face. Flupirtine injected i.p. dose-dependently prolonged the latency and reduced the amplitude of SEP with ID50-values of 5.4 mg/kg (2.6-9.3 mg/kg) and 7.9 mg/kg (3.9-13.8 mg/kg), respectively. This effect of flupirtine (10 mg/kg, i.p.) on the latency and the amplitude of SEP, did not change when naloxone (1 mg/kg, i.p.) was given before flupirtine. The results indicate that the analgesic flupirtine decreases the primary somatosensory evoked potential by diminishing the excitability of cortical neurons. Opioid mechanisms are not involved.     

The oxidation of N-2-Fluorenamine and 4-Aminobiphenyl by peracetic acid

Zusammenfassung Im Gegensatz zu neueren Befunden von anderen Autoren finden wir, dass die Oxydation von 2-Fluorenamin oder 4-Aminodiphenyl mit Peressigsäure keine Nitrosoderivate bildet, sondern die Azoverbindungen 2,2-Azoxybisfluoren und 4,4-Azobisdiphenyl.p-Nitroanilin wird zu einem Gemisch oxydiert, aus dem manp, p-Dinitroazoxybenzol undp, p-Dinitroazobenzol durch Chromatographie an Kieselgel isolieren kann.

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Supported by a grant (C-2571) from the National Cancer Institute, U.S. Public Health Service.