共查询到20条相似文献,搜索用时 15 毫秒
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Terminal sequences of bacteriophage RNAs 总被引:24,自引:0,他引:24
J E Dahlberg 《Nature》1968,220(5167):548-552
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多肽在丝状噬菌体外壳蛋白上的展示 总被引:1,自引:1,他引:1
王丽 《东北师大学报(自然科学版)》1998,(1):46-48
利用基因工程方法将丝状噬菌体fd基因8克隆入pKK233-3质粒中,将人工合成的六个氨基酸残基的多肽基因插入到修饰后的质粒载体中,并制备了杂合噬菌体。SDS-PAGE结果表明,外源多肽基因得到了正常表达,其产物展示于丝状噬菌体的外壳蛋白上。 相似文献
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刘彤 《大连海事大学学报(自然科学版)》1999,25(3):32-35
针对近年来应用于航海雷达的新型微带混频器,通过对输入输出信号频谱分析,严格论证了该器件的工作原理和工作特性,在理论和实践应用上具有参考价值。 相似文献
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对海稳定和地稳定矢量的优点和局限性作了全面地分析,并对雷达视角在避碰中的作用提出了不同看法。为了适应雷达在避碰和导航中的需要,对正确选用矢量稳定方式阐述了作者独到见解,并对ARPA性能指标提出了修改意见。 相似文献
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Radiochemical purification of bacteriophage lambda integrase 总被引:4,自引:0,他引:4
F M Ausubel 《Nature》1974,247(437):152-154
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海洋能源开发是海洋强国建设的重要支撑。论述了近年来科技创新为海洋能源勘探开发做出的巨大贡献,提出了中国海洋能源开发未来的主攻方向及建议:加大近海非常规油气开发技术攻关,为中国油气工业提供更多现实的贡献;加大深海油气勘探开发力度,打造中国油气产业未来的重要接替区;突破天然气水合物规模化开发关键技术,早日实现商业化开采;打造深海能源开发装备舰队,支撑海洋强国建设;抢占先机,布局并推进大洋矿产的勘探开发;进一步加强极地科研攻关,深度参与极地开发。 相似文献
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Recognition sequence of bacteriophage phi X174 gene A protein--an initiator of DNA replication 总被引:8,自引:0,他引:8
A D van Mansfeld S A Langeveld P D Baas H S Jansz G A van der Marel G H Veeneman J H van Boom 《Nature》1980,288(5791):561-566
Gene A protein, the initiator protein of bacteriophage phi X174 DNA replication, cleaves synthetic single-stranded oligodeoxyribonucleotides at the same site as the corresponding sequence at the phi X origin. The results identify the recognition sequence within the decamer CAACTTGATA which is cleaved next to the G residue. Further requirements for cleavage of double-stranded DNA by the gene A protein are supercoiling and an A + T-rich domain adjacent to the recognition sequence. 相似文献
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. 《科学通报(英文版)》2000,45(15):1434-1440
A new theoretical framework of tracer methods is proposed in the present contribution, on the basis of mass conservation.
This modet is applicable for both artificial and natural tracers. It can be used to calculate the spatial distribution patterns
of sediment transport rate, thus providing independent information and verification for the results derived from empirical
formulae. For the procedures of the calculation, first, the tracer concentration and topographic maps of two times are obtained.
Then, the spatial and temporal changes in the concentration and seabed etevation are calculated, and the boundary conditions
required are determined by fietd observations (such as flow and bedform migration measurements). Finally, based upon eqs.
(1) and (13), the transport rate is calculated and expressed as a function of the position over the study area. Further, appropriate
modifications to the modet may allow the tracer to have different densities and grain size distributions from the bulk sediment. 相似文献
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Boetius A Ravenschlag K Schubert CJ Rickert D Widdel F Gieseke A Amann R Jørgensen BB Witte U Pfannkuche O 《Nature》2000,407(6804):623-626
A large fraction of globally produced methane is converted to CO2 by anaerobic oxidation in marine sediments. Strong geochemical evidence for net methane consumption in anoxic sediments is based on methane profiles, radiotracer experiments and stable carbon isotope data. But the elusive microorganisms mediating this reaction have not yet been isolated, and the pathway of anaerobic oxidation of methane is insufficiently understood. Recent data suggest that certain archaea reverse the process of methanogenesis by interaction with sulphate-reducing bacteria. Here we provide microscopic evidence for a structured consortium of archaea and sulphate-reducing bacteria, which we identified by fluorescence in situ hybridization using specific 16S rRNA-targeted oligonucleotide probes. In this example of a structured archaeal-bacterial symbiosis, the archaea grow in dense aggregates of about 100 cells and are surrounded by sulphate-reducing bacteria. These aggregates were abundant in gas-hydrate-rich sediments with extremely high rates of methane-based sulphate reduction, and apparently mediate anaerobic oxidation of methane. 相似文献
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船舶液压传动系统故障诊断的理论研究 总被引:3,自引:0,他引:3
从液压传动系统的特征模型出发,提出了一种基于功率流理论的状态监测和故障诊断的方法、回路压降守恒规律和节点流量守恒规律。并以此为基础,通过一个典型的船舶机械液压传动系统,推导出全信息方程来确定最佳的监测点和故障位置。 相似文献
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发展海洋监测高新技术振兴海洋仪器行业 总被引:1,自引:0,他引:1
本文概述了我国海洋监测技术和仪器设备的发展现状,提出了建立和发展适合我国国情的海洋环境监测系统的必要性和重要性,从海洋监测系统建设、海洋仪器研制开发等方面分析了发展海洋监测新技术,振兴海洋仪器行业的意义以及必须解决的具体问题。 相似文献
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High-frequency generalised transduction by bacteriophage T4 总被引:30,自引:0,他引:30