基于β环糊精包合的激基缔合荧光

激基缔合荧光具有发射谱带宽、斯托克斯位移大的特点,在传感、检测、成像及照明等方面具有广泛应用。本文提出了一个基于β-环糊精（β-CD）包合作用构建激基缔合发光体的新方法。通过多环芳醛（蒽醛和芘醛）与金刚烷胺缩合合成了2个席夫碱（ANAD和PYAD）,二者都显示激基缔合增强的聚集诱导发光。席夫碱的金刚烷基与β-CD的疏水空腔高度匹配,与β-CD形成包合物后荧光性质会发生明显变化。在β-CD水溶液中,ANAD的发射光谱与β-CD浓度密切相关,当c（ANAD）∶c（β-CD）为1∶2时观察到明显的激基缔合发射。与ANAD不同,PYAD在各种浓度的β-CD水溶液中都发射激基缔合荧光,但过量的β-CD使荧光强度有所减弱。在固态下,ANAD和PYAD几乎没有荧光,与β-CD形成包合物后,二者都显示强激基缔合发射。总之,与β-CD形成包合物更有利于发射激基缔合荧光。     

微流控芯片加工工艺及生物相容性探究

本文就实验室微流控玻璃芯片湿法刻蚀加工工艺进行了研究,并对加工后芯片表面的生物相容性进行了评估.结果该工艺展宽100 μm,H₂SO₄处理后的基质生物相容性更好.所以在涉及具体的操作前,有必要进行相应的清洗以及包被处理,以便后续实验顺利开展.对于微流控芯片相关实验具有借鉴与指导意义.     

催泪喷射器中刺激组分的紫外分光光度法分析

采用紫外分光光度法对催泪喷射器中的刺激组分邻氯苯亚甲基丙二腈(CS)和辣椒素(OC)进行检测.分别选取300 nm和281 nm作为CS和OC的紫外检测波长,通过外加标准物质定量法建立标准曲线,对催泪喷射器中刺激组分进行含量检测,并依据HPLC方法测定结果对比检测可靠性.本方法对邻氯苯亚甲基丙二腈和辣椒素的检出限分别为0.02 μg·mL^-1和0.16 μg·mL^-1,精密度分别为1.04%和0.64%,平均回收率分别为97.37%和102.51%,CS和OC分别在0.1~25.0 μg·mL^-1和1~200 μg·mL^-1范围内具有良好的线性关系,线性相关系数均为0.999 9.结果表明,该方法简单、快速、成本低,可用于催泪剂中刺激组分的含量分析.     

豌豆根瘤菌gshR基因的抗氧化和共生固氮作用

目的: 研究豌豆根瘤菌 RL3841 的谷胱甘肽还原酶编码基因 gshR 的功能．方法: 采用基因同源重组构建了豌豆根瘤菌 gshR 基因突变株 RLgshR,探讨了基因突变对根瘤菌抗氧化和共生固氮的影响, 利用实时荧光定量 RT-PCR检测 gshR基因的 mRNA 表达水平．结果: gshR基因缺失不影响菌株在 AMS 基础培养基中的生长能力, 但突变株对氢过氧化枯( CuOOH) 和较高浓度的 H₂O₂氧化物敏感．结论: gshR 基因突变株 RLgshR 形成正常固氮根瘤, gshR 基因的表达不受 H2O2和共生环境诱导．     

乙型肝炎病毒HBx基因的shRNA重组表达载体的构建和鉴定

目的: 构建 pGenesil-3.1-HBx-shRNA 重组表达载体, 探讨其作用于 HepG2.2.15 细胞的生物活性． 方法: 构建了针对HBx基因的siRNA 表达载体,通过电穿孔法转染 HepG2.2.15 细胞,以荧光显微镜观察细胞的转染效率,QRT-PCR检测细胞中HBx基因的相对表达量,利用CCK-8细胞增殖检测试剂盒检测了细胞增殖活力,流式细胞术检测细胞凋亡．结果: 酶切和测序鉴定证实构建质粒含有HBx基因,QRT-PCR检测和荧光显微镜观察到构建的pGenesil-3.1-HBx-shRNA 可以在 HepG2.2.15 细胞中表达,HBx基因的表达下降了 28%(P＜0.05),细胞增殖水平下降了47%(P＜0.05),质粒 pGenesil-3.1-HBx-shRNA 转染后的HepG2.2.15 细胞凋亡率显著升高．结论: 乙型肝炎病毒HBx基因的shRNA重组表达载体构建成功,沉默HBx基因的shRNA能够抑制HBx基因的表达,且对HepG2.2.15细胞的增殖水平起明显的抑制作用．     

大粒径石灰石高温分解动力学

构建符合炼钢溅渣护炉阶段和开吹阶段在转炉中直接投入石灰石代替石灰的模型,对不同粒径、不同传热条件下的大粒径石灰石进行高温分解实验。通过实验求得石灰石分解转化率α,选取适合的反应控速环节,对不同控速环节进行分析,研究溅渣护炉阶段和开吹阶段大粒径石灰石等温加热动力学机理。结果表明：石灰石转化率随粒径增加减小,随温度升高增大;溅渣护炉阶段界面化学反应控速,分解模式为随机成核与随后生长模式,符合Avrami-Erofeev方程,最概然函数积分形式为：G（α）=[-ln（1-α）]ⁿ,n=3/4;开吹阶段产物层传热与CO₂扩散控速,温度一定时,时间t和（1-α）ln（1-α）+α<之间存在线性关系。     

纸基检测方法研究进展

随着现代检测技术的简约化、快速化、低成本的发展趋势,基于纸的各类检测方法应运而生.纸作为一种新的检测载体,既能满足传统检测方法的基本要求,还具备了便捷、成本低、检测时间短、适合户外及发展中国家实验室需求等优点.近年来纸基检测发展迅速,在酶联免疫吸附测定法、微流控检测、电传感器、荧光检测、核酸检测等方法中应用广泛.分析并梳理纸基检测方法在以上领域存在的不足,展望其在蛋白质检测、生物标志物筛查和临床诊断中的应用前景,对后续研究具有重要意义.     

混合梁刚构桥受力计算方法与合理结构体系研究

在传统混凝土刚构桥基础上把主跨跨中的一部分改为钢梁而形成的混合梁刚构桥有效实现了梁桥在跨度上的突破及结构性能的改善,成为非常有应用前景的一种桥梁结构形式,并在实际工程中越来越被广泛应用。为探究三跨钢-混凝土混合梁刚构桥结构体系的合理布置和受力性能的简化计算方法,推导了混合梁刚构桥在施工阶段及成桥阶段的简化力学模型,并通过有限元方法和实桥测试验证了简化力学模型的正确性。基于简化力学模型,以控制截面内力为目标函数,计算分析了边跨与中跨长度之比λ、钢梁长度与主跨长度之比μ（或结合段位置参数）对混合梁刚构桥受力性能的影响。研究结果表明,基于结构的合理受力性能,得到了λ和μ的合理取值范围,综合考虑桥梁施工和运营中的风险,建议λ取0.4、μ取0.4~0.5,可作为混合梁刚构桥的设计参考。     

荧光分析法测定美洲大蠊中多肽含量研究

荧光分析法是一种经济快速的分析方法,具有灵敏度高、选择性好、操作简单等优点。鉴于美洲大蠊多肽（DPSFNSWG-NH₂）自身具有荧光的特性,通过实验建立一种美洲大蠊多肽（DPSFNSWG-NH₂）的荧光检测新方法。实验结果表明,在最佳条件下,美洲大蠊多肽（DPSFNSWG-NH₂）在0.12 ~ 31.25 μg/mL浓度内与荧光强度之间具有良好的线性关系,线性回归方程为y = 10.698x + 14.625,R²= 0.994 2,检测限为0.09 μg/mL,将所建立的新方法用于美洲大蠊药材中其多肽（DPSFNSWG-NH₂）的测定,回收率为85.50% ~ 89.47%。本研究方法操作简便、快速、准确,具有良好的分析应用前景,可用于美洲大蠊中DPSFNSWG-NH₂多肽的含量测定。     

Design of electric-field coupled underwater wireless power transfer system based on class E amplifier

针对单级LC谐振型电场耦合式水下无线电能传输(electrical-field coupled power transfer,ECPT)系统的输出功率低、谐振容量小、频率漂移大等关键问题,设计一种更适于海水环境下的无线电能传输系统。该系统基于CLC-S调谐网络,综合E类放大器效率高、频率高等优点,实现较大的功率输出。利用Maxwell有限元仿真软件,对海水环境下耦合机构的电容值进行了有限元分析。通过对水下耦合机构进行试验,分析负载品质因数Q、归一化频率μ对水下无线电能传输系统的影响规律,可为水下ECPT系统设计提供参考。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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