Transmission of Rauscher Leukemia in mice

Zusammenfassung Es wird gezeigt, dass die wirkungsvollste übertragung desRauscher-Leukämie-Virus vertikal durch die Milch geht. Eine transplazentale übertragung wurde nicht erreicht. Die horizontale übertragung hingegen war unter optimalen Bedingungen minimal.     

Actions of interferon in tissue cultures harboring mouse leukemia virus

Zusammenfassung Das Virus der vesikulären Stomatitis verursacht Zellverschmelzung und Syncytienbildung in mit Mäuse-Leukämie-Virus infizierten Gewebskulturen. Interferon konnte diese Zellzerstörung in der Kultur nicht verhindern.

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This work was supported by U.S.P.H.S. research grant No. CA-7923 and award No. K3-CA-16747.     

Propagation of mouse hepatitis virus (MHV-3) in monolayer cell cultures from liver of new-born mice

Riassunto È stata provata la possibilità di coltivare MHV-3 in colture monostrato di cellule di fegato di topo neonato ottenute per tripsinizzazione. Per questo tipo di coltura è indispensabile l'aggiunta alla tripsina di metilcellulosa e di estratto embrionale di pollo al terreno di coltura. MHV-3 si moltiplica attivamente in queste colture, raggiungendo elevate concentrazioni nel liquido di coltura e producendo effetto citopatico.     

Inhibition of virus multiplication and alteration of cyclic AMP level in cell cultures by flavonoids

The inhibitory effect of four flavonoid compounds on virus multiplication and their influence on the intracellular cyclic AMP (cAMP) level were studied in cell cultures. Quercetin and quercitrin reduced the yields of Human (alpha) herpesvirus 1 (HSV-1) and Suid (alpha) herpesvirus 1 (pseudorabies virus), but hesperidin and rutin had no effect. Further, quercetin and quercitrin elevated the intracellular level of cAMP, whereas hesperidin and rutin did not alter the cAMP level. Both antiviral activity and cAMP-enhancing effect were dependent on the concentrations of the flavonoids, and these effects turned out to be parallel. This study suggests that a relation exists between the antiviral effect and the cAMP-enhancing activity of flavonoids.     

Inhibition of virus multiplication and alteration of cyclic AMP level in cell cultures by flavonoids

Summary The inhibitory effect of four flavonoid compounds on virus multiplication and their influence on the intracellular cyclic AMP (cAMP) level were studied in cell cultures. Quercetin and quercitrin reduced the yields ofHuman (alpha) herpesvirus 1 (HSV-1) andSuid (alpha) herpesvirus 1 (pseudorabies virus), but hesperidin and rutin had no effect. Further, quercetin and quercitrin elevated the intracellular level of cAMP, whereas hesperidin and rutin did not alter the cAMP level. Both antiviral activity and cAMP-enhancing effect were dependent on the concentrations of the flavonoids, and these effects turned out to be parallel.This study suggests that a relation exists between the antiviral effect and the cAMP-enhancing activity of flavonoids.     

Repressed chromatin from virus infected and from differentiated cells

Zusammenfassung Aus Virus-infizierten Zellen isoliertes Chromatin wurde mit dem Erythrocytenchromatin verglichen. Die durch Infektion oder Differenzierung verlorene Kapazität des Chromatins der RNS-Syntheseunterstützung wird durch Entfernung eines Teiles des Proteins wieder hergestellt.

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This work was supported by the Cancer Research Fund of the University of California and by U.S.P.H.S. Grant CA-08192-1.