In vitro and in vivo catabolism of cholesterol in meal-fed rats

Zusammenfassung Bei männlichen Wistar-Ratten wurde der Einfluss des meal-feeding-Regimes nach täglicher 2stündlicher Fütterung an Leberschnitten auf den Katabolismus von Cholesterol-4-¹⁴C geprüft und normal befunden. Im In-vivo-Versuch unter denselben experimentellen Bedingungen zeigte weder der Katabolismus noch die Exkretion des i. p. applizierten Cholesterol-4-¹⁴C eine Veränderung.     

Inhibition of deiodination of diiodotyrosine in vivo; relation to catecholamine biosynthesis

Résumé La diiodotyrosine est un puissant inhibiteur in vitro de l'enzyme limitant la synthèse des catécholamines, la tyrosine hydroxylase. Or, administrée in vivo à des rats, la diiodotyrosine n'inhibe que très peu la production endogène de catécholamines. Cependant, en ralentissant la rapide désioduration de la diiodotyrosine injectée par le ménadione, on observe une inhibition marquée de la synthèse des catécholamines in vivo.     

In vivo and in vitro molecular hybridization of malate dehydrogenase isozymes

Résumé Les hybrides moléculaires des isozymes de la déshydrogénase de malate de différentes espèces de poissons peuvent être engendrés in vitro en gelant puis dégelant les isozymes en présence du sel. L'isozyme hybride est identique à celle observée in vivo dans l'hybride interspécifique F₁.     

In vivo and in vitro effect of sulfathiazole on serum glycoproteins

Zusammenfassung Durch Sulfathiazolzugabe in vitro oder durch Sulfathiazolbehandlung in vivo wird der Glycoproteingehalt des Albumins und der-1-Globuline reduziert.     

In vivo and in vitro differences between leukocytic uptake of oligodeoxynucleotides

Development and application of therapeutic oligonucleotides rely on proper analysis of binding and uptake. We have used several model oligodeoxynucleotides (ODNs) to analyze binding/uptake by rat and human leukocytes. Here we describe: (1) differences between in vivo and in vitro uptake of ODNs to rat leukocytes, (2) differences after injection of lipopolysaccharide (LPS), (3) large in vitro differences between primary mononuclear cells in PBS, plasma and blood, and (4) differences of ODN uptake between rat and human leukocytes. Our data show that ODN uptake by primary blood cells was different in PBS, plasma and blood. In addition, LPS treatment increased ODN uptake by leukocytes in blood, indicating that pathological conditions may influence ODN uptake. Furthermore, ODN uptake in rat and human blood is also different, suggesting that preclinical ODN uptake data from rat blood cannot easily be extrapolated to the human condition. Received 17 December 2007; received after revision 16 January 2008; accepted 5 February 2008     

In vitro and in vivo interaction of nuclear antibodies with corresponding antigens

Zusammenfassung Es wird mit indirekter Immunofluoreszenz gezeigt, dass Leberzellkerne mit Antikörpern bedeckt werden können, wenn sie systemischemLupus erythromatosus-Serum (SLE) für weniger als 2 sec ausgesetzt werden, und dass die Färbungsintensität vom Titer des Antikörpers abhängt.Diese Ergebnisse deuten darauf hin, dass das in SLE-Geweben durch direkte Immunofluoreszenz nachgewiesene nukleare-Globulin hauptsächlich nukleare post-Biopsiereaktionen wiederspiegelt und weniger in vivo-Reaktionen, und dass in beiden Fällen nur abgestorbene oder nicht intakte Zellen vom Antikörper durchdrungen werden.

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This investigation was supported in part by a training grant (2E-130) from the U.S. Public Health Service. Taken in part from thesis (G.W.B.) submitted to the Faculty of the Graduate School of Arts and Sciences of the University of Buffalo (N.Y., USA) in partial fulfillment of the requirements for the degree of Doctor of Philosophy (1962). Present address (G.W.B.): Department of Pathology, State University of New York at Buffalo.     

In vivo and in vitro incorporation of 3H-thymidine in the uterus of the rabbit during pseudopregnancy

During pseudopregnancy in the Rabbit the DNA synthesis in the uterine epithelium is very reduced. These results conjoined with the decrease of the mitotic index and previous ultrastructural observations allow to think that multinucleated cells proceed mainly by cell fusion.     

In vitro and in vivo inhibitory action of 2-amino-4,6-dichloropyrimidine on polio and herpes virus

Résumé La 2-amino-4,6-dichloropyrimidine (Py 11) est capable d'inhiber la réplication du poliovirus et du virusHerpes simplex dans des milieux de culture complète, à condition que la mercaptoéthanolamine y soit aussi présente. Les combinaisons des deux composés sont actives, in vivo contre l'herpes cornealis du lapin.

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This work has been supported by a Grant of the Consiglio Nazionale delle Ricerche, Roma.     

In vitro andin vivo activity on catalase of electrophoretically pure human serum albumin

Riassunto Albumine umane purissime dimostranoin vivo sulla catalasi epatica dei topi, una inibizione molto significativa fino alla dose di 10 mg/topo;in vitro, sulla catalasi cristallizzata, determinano un certo aumento dell'attività di difficile interpretazione.

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Acknowledgment. The present research was partially supported by a grant from Farmitalia S.p.A.