Pseudolymphoma of skin induced by oriental hornet (Vespa orientalis) venom.

Intradermal injection of saline suspension of Vespa orientalis venom sac to 57 black mice caused a local nodule composed of lymphocytes, few histiocytes and plasma cells 10 to 12 days following the injection. This reaction simulates the pseudolymphoma reaction observed in humans following arthropode stings.     

Über den Einfluss von Desoxycorticosteron auf Kontraktur und Kaliumfreisetzung durch Acetylcholin an der innervierten und denervierten Skelettmuskulatur

Summary The release of potassium ions from striated muscle, and the changes in mechanical tension developed by the gastrocnemius muscle on intraarterial injection of ACh, were investigated by isolated perfusion of the hind limbs in cats. The reaction of the normal innervated and chronically denervated muscle in the same animal were compared before and during perfusion with 2·10^–4 M/l desoxycorticosterone glucoside (DCG). The following results were obtained: after perfusion with DCG no change in the spontaneous release of potassium ions occurred neither on the innervated nor on the denervated muscle. The potassium release following intra-arterial injections of various doses of ACh was significantly reduced on innervated and denervated muscle. On the denervated muscle there was also a considerable reduction of the height of contractures caused by ACh administration. The results make it probable that DCG acts by an inhibition of depolarisation in the same way asd-tubocurarine.     

Disparition de la 5-hydroxytryptamine dans les granulations des glandes de la peau deDiscoglossus pictus et deRana temporaria après injection de réserpine

Summary 30 min after an intraperitoneal injection of reserpine (5 or 10 mg/kg) in the frog (Rana temporaria) and in the toad (Discoglossus pictus), a substance which has the properties of 5-hydroxytryptamine (argentaffine reaction, azoreaction, indoreaction and fluorescence in U.V. after formaldehyde) disappears from the skin glands; the histochemical reactions are more positive again 1 1/2 to 2 h after the injection of reserpine.     

Hydroxyprolin-Ausscheidung im Rattenharn nach Injektion von Schlangengift

Summary In rats, a statistically significant increase in hydroxyproline excretion occurs following injection of crotalus toxin (Agkistrodon piscivorus Lacépède). The significance of this finding is briefly discussed.     

Comportamento dell allossanain vivo

Summary The author describes a microprocedure, based on the o-phenylenediamine reaction, for the determination of alloxan in blood and tissuesin vivo. With this method he has been able to determine alloxan in blood, liver, kidney, muscle, and pancreas of rats treated with diabetogenous doses of alloxan. This substance has been found in the blood and tissues during an hour and a half after the injection and in the urine collected directly from the ureter a quarter of an hour after the injection.     

3-Ethyl-2,5-dimethylpyrazine,a component of the trail pheromone of the antMessor bouvieri

Summary 3-Ethyl-2,5-dimethylpyrazine, found in the poison gland of workers, induces trail following in the Mediterranean harvester antMessor bouvieri. The poison gland contains on average 9 ng of this trail pheromone component. The alkaloids anabasine and anabaseine are also present in this gland, but induce no reaction in workers. Trail following is also induced by substances present in the Dufour gland, but the combined effect of both glands shows no synergism.     

Bildung von Reaktionsholz durch Zentrifugalbeschleunigung

Summary In leaning stems, reaction wood can be formed as a response to gravitation stimulus. In seedlings ofAesculus hippocastanum, reaction wood formation could likewise be stimulated by centrifugation. This proved the differentiation of wood cells to be affected by mass acceleration. The reaction wood formation is related quantitatively to mass accelerationb and timet, the formation of reaction wood being described by quantitative stimuli law as . The threshold of stimulation lies in the dimension . The arrangement of the experiments permits a direct approach to cambium physiology and the following differentiation of wood cells.     

Hemolymph proteins in an ascitic condition,induced by lethal1(3)gl tumorous tissue inDrosophila hydei

Summary Drosophila hydei larvae homozygous for the gene1(3)gl may survive to the age of 3 weeks, become bloated and be incapable of metamorphosis. Wild-type flies after the injection of a fragment of the1(3)gl disc, which has been previously culture in vivo for 40–500 days, also become bloated (ascitic reaction). In the hemolymph of both the advanced1(3)gl larvae and ascitic adult hosts the accumulation of a specific protein is observed.Acknowledgments. This work was supported by the Swiss National Science Foundation under Grant No.3.792-0.76 and the Georges und Antoine Claraz-Schenkung. We thank Prof. H. Gloor, University of Geneva, and Prof. P.S. Chen, University of Zürich, for advice and criticism.     

Polyvinylchloride (PVC) particles implantation in mouse liver. A technique for experimental study of schistosome eggs-induced liver pathology

Summary An injection of suspended PVC particles in the caecal vein of mice induces a foreign-body portal granuloma reaction in the liver. Plastic casts of the portal system, after PVC particles implantation, show modifications in the portal bed and are compared with plastic casts obtained in mice infested bySchistosoma mansoni. This technique can be useful to study the cellular dynamics of the portal granuloma and can be a model for schistosomal eggs induced liver pathology.This work was supported by an ATP (INSERM) 18.75.41 and a scholarship from the Société d'Hépatologie Expérimentale.     

Presenza di un principio antidiuretico nelle ghiandole salivari posteriori degli Octopodi

Summary Acetone and alcohol extracts of the posterior salivary glands ofOctopus vulgaris, Eledone moschata andEledone Aldrovandi contain a principle which strongly reduces the diuresis in hydrated rats.The antidiuretic principle is absent in all otherOctopus tissues as well as in the salivary glands ofOctopus macropus.It shows a high termostability and is strongly adsorbed by animal charcoal at any reaction.The salivary antidiuretic substance is not identical with the posterior pituitary principle nor with the other active substances found in the salivary glands of Octopoda.The injection of salivary extracts satisfactorily reduces polyuria and polydipsia in human diabetes insipidus.

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Le presenti ricerche sono state in parte sovvenzionate da un contributo straordinario del Consiglio Nazionale delle Ricerche.     

Das Na-Transportsystem während der Erregungsprozesse am Ranvier-Knoten isolierter markhaltiger Nervenfasern

Summary (1) An attempt is made to interpret the action potential of medullated nerve fibres of the frog in terms of permeability changes to Na ions.(2) In keeping with a suggestion byHodgkin andHuxley, the following assumptions are made: (a) a carrier system is responsible for transporting Na ions across the nodal membrane; (b) in a resting fibre (high membrane potential) its transport capacity is small; (c) depolarization has a dual effect on the transport system, namely activation of carrier groups as a quick reaction and inactivation of the whole system as a slower reaction.(3) The following experimental observations are interpreted on the ground of these assumptions: threshold for depolarization, threshold for repolarization, absolute and relative refractory period, alteration of the action potential on application of a hypertonic (5%) NaCl solution.

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Eine vorläufige Mitteilung erfolgte auf der Tagung des Schweiz. Vereins für Physiologie usw. in Fribourg 1956. Helv. physiol. Acta14, C35–36 (1956).     

Microinjection of synthetic protein kinase inhibitor into single barnacle muscle fibers before and after cyclic AMP

Summary Single muscle fibers from the barnacleBalanus nubilus have been used as a preparation to see if a synthetic 20-residue PKI (5-24)-peptide is able to block or reverse the stimulatory response of the ouabain-insensitive Na efflux to injected cAMP. The results obtained show that this peptide behaves as a powerful inhibitor of the cAMP-mediated response and is also able to partially reverse the sustained stimulation of the Na efflux observed in ouabain-poisoned fibers following the injection of subunit A of cholera toxin.Warmest thanks to Prof. E. H. Fischer and Kurt Diltz for a sample of sPK1. This work received support from NSF, the Graduate School Research Committee and from NIH through a General Research Support Grant to the University of Wisconsin Medical School.     

Increased resistance in mice to Listeria monocytogenes after treatment with a fraction from an inflammatory granuloma]

The multiplication of Listeria monocytogenes in the spleen was decreased between the 3rd and the 11th day following the inflammatory reaction induced in Mice by subcutaneous implantation of talc embedded in a calcium phosphate gel into the dorsal area. A similar activity was observed after injection of SO4 (NH4)2 fractionated extracts from granuloma. The major activities were found in the 33% saturation precipitate and in the 80% saturation supernatant.     

Endotoxin-induced acceleration of ovum transport in rabbits

Summary Salmonella enteritidis-Boivin endotoxin (1–20 g/kg) induced accelerated oviductal ovum transport in rabbits in a dose-related manner. Indomethacin prevented this effect. Levels of prostaglandin E and F in uterine vein blood increased following endotoxin injection.Deceased 6 December, 1977.Thanks are due to Elizabeth Menchaca, Larry Coons and Lenore Weinberg for technical assistance, and to Merck, Sharp, and Dohme for the gift of indomethacin. This work was supported by Rockefeller Foundation, NIH (HD-09339) and WHO (S76119). G. V. is a Rockefeller Foundation Postdoctoral Fellow in Reproductive Biology. Reprint requests to M. J. K. Harper.     

Haemolymph ecdysteroids level following the injection of ecdysone or ecdysterone; its relation with tegument and midgut response inAeshna cyanea (Insecta,Odonata)

Summary The haemolymph ecdysteroid level after injection of ecdysone or ecdysterone inAeshna cyanea larvae has been determined by a radioimmunoassay method. The rate of excretion appears to be dependent on both the ecdysteroid injected and the time of injection. In case of ecdysone injection, the secretion of the epidermis cuticle and the differentiation of the imaginal midgut epithelium occur when the ecdysteroid level remains low for many days.     

Einfluss von augenstielextrakt auf trehalose,fructose und glucose in der hämolymphe vonOrconectes limosus

Summary Quantitative changes of glucose, fructose and trehalose in the hemolymph ofOrconectes limosus were measured after injection of eyestalk extracts. Especially in February and March high levels of trehalose were found after hormone injection.     

Asymmetry of turning behaviour of rats induced by amphetamine and apomorphine

Summary Turning behaviour of rats following injection of amphetamine is in the same direction as following apomorphine. Unilateral common carotid artery ligation does not affect this asymmetry. Thus the reason for the asymmetry in turning behaviour following injection of amphetamine is probably due to postsynaptic dopaminergic asymmetry within the corpus striatum.     

Über den zeitlichen Ablauf der Aktivierung von Cyclophosphamid im Menschen und die Testung der zytostatisch aktiven Form an Ehrlich-Mäuse-Aszites-Tumorzellen

Summary The cytostatic action of human blood serum, following intravenous injection of cyclophosphamid, on cell proliferation and lactic production of Ehrlich mouse ascites tumour cells in vitro was tested. The maximum cytostatic activity was found 2 h after injection.     

Distribuzione di fosfatasi alcaline in gonadi di Crostacei isopodi in rapporto al ciclo degli acidi nucleici

Summary The distribution of alkaline phosphatases has been studied in male and female gonads of some isopod crustaceans by means of Gomori's reaction.Monophosphatases are found on all Feulgen-positive nuclear structures, in the nucleolus and in the cytoplasm of young oocytes. They disappear from the cytoplasm in auxocytosis, during yolk formation, although they persist in the germinal vesicle. The authors think that the cytoplasmic localization is coincident with ribonucleic acid distribution.From the experiments related in the following note, nucleolar phosphatases appear to be in some way different from the nuclear (sensu stricto) and cytoplasmic phosphatases.Diphosphatases do not follow so closely the nucleic acid localization. They are absent from cytoplasm; in the nucleolus they are not distributed uniformly but show characteristic structure; in the nucleus they are not localized in the chromosomes but homogeneously diffused.