红车轴草提取物对小鼠T淋巴细胞体外活化与增殖的影响

目的:探讨红车轴草提取物(Trifoliumpratense Leguminosaeextract,TLE)对小鼠T淋巴细胞的体外活化和增殖的影响.方法:MTT比色法检测TLE对细胞的毒副作用及增殖抑制情况;双色荧光抗体染色结合流式细胞术分析TLE对小鼠T淋巴细胞在多克隆刺激剂(ConA)刺激下体外活化抗原CD69表达的影响;羧基荧光素乙酰乙酸(carboxyfluorescein diacetate,succinimidyl ester,CFDA-SE)标记技术结合流式细胞术,分析CD3 T淋巴细胞增殖相关指数.结果:TLE对小鼠淋巴结来源的淋巴细胞微毒副作用;终质量浓度为10、20、30、40 mg/L的TLE对ConA刺激下的T淋巴细胞体外活化抗原CD69表达具有明显的抑制作用(P＜001),40 mg/L抑制达到峰值;MTT法和CFDA-SE染色法都显示,10、20、30、40 mg/L TLE对ConA诱导的T淋巴细胞增殖具有显著的抑制作用(P＜001).     

鹰嘴豆芽素A对小鼠T淋巴细胞体外活化增殖和细胞周期的影响

目的:研究黄酮类化合物鹰嘴豆芽素A(biochanin A,bioA)对小鼠T淋巴细胞的活化增殖和周期的影响.方法:运用双色荧光抗体染色技术结合流式细胞术分析bioA对小鼠T淋巴细胞在多克隆刺激剂(ConA)刺激下早期活化抗原CD69表达的影响;运用羧基荧光素乙酰乙酸(carboxyfluorescein diacetate,succinimidyl ester,CFDA-SE)标记技术结合流式细胞术,分析T淋巴细胞增殖相关指数;用碘化丙锭(propidium iodide,PI)染色分析T淋巴细胞细胞周期变化.结果:终浓度为25、50μmol/L的bioA对ConA刺激下的T淋巴细胞早期活化抗原CD69表达具有明显的抑制作用(P＜0.05),50 μmol/L抑制达到峰值;CFDA-SE染色法显示,25、50 μmol/L bioA对ConA诱导的T淋巴细胞增殖作用具有显著的抑制作用(P＜0.05);终浓度为5、25、50 μmol/L的bioA能够使ConA刺激的T淋巴细胞滞留于G0/G1期,减少处于S期和G2/M期细胞数.结论:BioA对小鼠T淋巴细胞的活化增殖和周期具有明显的抑制作用.     

穿心莲内酯对小鼠T淋巴细胞体外活化、增殖及细胞周期的影响

目的:研究穿心莲内酯对小鼠T淋巴细胞体外活化、增殖及细胞周期的影响,并对其免疫调节作用进行初步探讨.方法:分离小鼠淋巴结细胞,以双色荧光抗体染色技术结合流式细胞术检测在刀豆蛋白A(Con A)的刺激下,穿心莲内酯对T淋巴细胞早期活化抗原CD69表达的影响;用羧基荧光素乙酰乙酸(CFDA-SE)染色分析穿心莲内酯对T淋巴细胞增殖的影响;用碘化丙锭染色分析细胞周期的分布.结果:终浓度为10、20、30、40 μmol/L的穿心莲内酯对Con A刺激的早期活化抗原CD69的表达具有明显的抑制作用(P<0.01);对Con A刺激的T淋巴细胞增殖具有明显的抑制作用(P<0.01);并明显阻止T淋巴细胞进入S期,且呈剂量依赖性.结论:穿心莲内酯对小鼠T淋巴细胞的体外活化及增殖均具有明显的抑制作用,并抑制T淋巴细胞进入分裂周期.     

细胞外信号调节激酶1/2信号通路在小鼠T细胞增殖、周期和活化中的作用

目的:探讨细胞外信号调节激酶1/2(ERK1/2)信号通路在小鼠T淋巴细胞增殖、周期和活化中的作用.方法:分离小鼠淋巴结细胞,藉多克隆刺激剂刀豆蛋白(ConA)或佛波醇酯(PDB)加离子霉素(Ion)刺激,流式细胞术分析ERK1/2信号通路的特异性阻断剂PD98059对小鼠T淋巴细胞增殖、周期和活化的影响.结果:活体染料羧基荧光素乙酰乙酸染色分析显示,不同浓度(5、10、20、30、40 μmol/L)的PD98059对ConA诱导的T淋巴细胞增殖具有明显的抑制作用,呈现剂量依赖关系(r=0.985,P＜0.01).碘化丙锭染色分析表明,PD98059可阻止ConA刺激的T淋巴细胞进入S期和G2/M期,PD98059对PDB Ion刺激的T淋巴细胞细胞周期的影响与ConA刺激相似,不同的是S期和G2/M期的变化较ConA作用更显著.荧光标记单克隆抗体染色显示,不同浓度的PD98059仅能轻微影响T淋巴细胞表面活化标志CD69和CD25的表达.结论:PD98059对小鼠T淋巴细胞的增殖有明显抑制作用,并阻止其进入S期和G2/M期,但不能明显抑制小鼠T淋巴细胞的早期和中期活化.     

九种黔产植物多糖的体外免疫调节活性研究

目的:评价9种黔产植物多糖的免疫调节作用。方法制备脾淋巴细胞悬液,分别将样品与LPS或ConA共同作用于脾淋巴细胞,用MTT法测定淋巴细胞的增殖情况。将多糖作用于RAW264.7细胞,用ELISA测定细胞上清中TNF-α含量,判断样品对RAW264.7细胞的活化情况。结果:土党参多糖、薯蓣多糖、竹根假万寿竹多糖对LPS刺激B淋巴细胞增殖有促进作用。楮实多糖对ConA刺激T淋巴细胞的增殖有促进作用。轮叶沙参多糖、绞股蓝多糖、党参多糖、金樱子多糖对T、B淋巴细胞增殖均有促进作用。而绞股蓝多糖、薯蓣多糖、金樱子多糖、楮实多糖、竹根假万寿竹多糖能诱导RAW264.7细胞TNF-α表达上调。结论:九种黔产植物多糖在促淋巴细胞增殖和巨噬细胞活化中表现出明显差异,其中绞股蓝多糖有较好的免疫增强活性。     

米诺环素对PDB刺激的小鼠淋巴细胞体外活化和增殖的影响

为了分析米诺环素(MNC)对小鼠淋巴细胞体外活化和增殖的影响,探讨其免疫抑制作用机制.我们通过分离小鼠淋巴结细胞,以佛波醇酯(PDB)刺激,用不同终浓度的MNC与淋巴细胞共培养,荧光抗体染色结合流式细胞术,检测淋巴细胞早期和中期活化抗原CD69与CD25的表达;以CFSE染色,加入多克隆刺激剂佛波醇酯(PDB)和离子霉素(Ion)进行刺激,以流式细胞仪分析MNC对淋巴细胞增殖的影响;结果,荧光抗体染色显示MNC能显著抑制PDB诱导的T细胞CD69和CD25的表达;CFSE染色结果显示,MNC对佛波醇酯(PDB)和离子霉素(Ion)诱导48 h的小鼠淋巴细胞增殖,具有明显的抑制作用(P<0.05),且呈剂量依赖性.从而我们认为MNC对小鼠淋巴细胞的体外活化和增殖具有明显的抑制作用.具有独立于其抗菌活性的抗炎作用.     

C_2-神经酰胺对小鼠T淋巴细胞体外活化和增殖的影响

目的:研究C2-神经酰胺(C2-cer)对小鼠T细胞体外活化和增殖的影响,并对其免疫调节作用进行初步探讨.方法:分离小鼠淋巴结细胞,加入刀豆蛋白A(ConA)或佛波醇酯(PMA)和离子霉索(Ion)进行刺激,以不同终浓度的C2-cer与T细胞共培养.流式细胞术结合双色荧光抗体染色,检测T细胞早期活化抗原CD69的表达;用羧基荧光素双醋酸盐琥珀酰(CFDA-SE)染色结合流式细胞术,并用ModFit软件分析T细胞增殖相关指数.结果:终浓度为25、50和75 μmol/L的C2-cer可以显著抑制ConA诱导的T细胞CD69的表达,由ConA组的(60.13±1.18)%,分别降低为(54.56±1.14)%、(48.73±1.26)%和(27.09±1.07)%(P＜0.01);CFDA-SE染色结果显示,各浓度C2-cer对ConA诱导的小鼠淋巴细胞增殖,具有明显的抑制作用,增殖指数(PI)由ConA组的(1.81±0.25),分别降低为(1.46±0.01)、(1.25±0.04)和(1.18±0.03)(P＜0.05);各浓度C2-cer均可明显抑制PMA+Ion诱导的小鼠淋巴细胞增殖,增殖指数(PI)由PMA+Ion组的(1.47±0.01),分别降低为(1.27±0.01)、(1.11±0.01)和(1.05±0.01)(P＜0.05).结论:C2-cer能有效地抑制小鼠T细胞的体外活化和增殖.     

In vitro screening of immunomodulatory activity of polysaccharides of nine species of plants in Guizhou province

目的：评价9种黔产植物多糖的免疫调节作用。方法制备脾淋巴细胞悬液,分别将样品与LPS或ConA共同作用于脾淋巴细胞,用MTT法测定淋巴细胞的增殖情况。将多糖作用于RAW264.7细胞,用ELISA测定细胞上清中TNF-α含量,判断样品对RAW264.7细胞的活化情况。结果：土党参多糖、薯蓣多糖、竹根假万寿竹多糖对LPS刺激B淋巴细胞增殖有促进作用。楮实多糖对ConA刺激T淋巴细胞的增殖有促进作用。轮叶沙参多糖、绞股蓝多糖、党参多糖、金樱子多糖对T、B淋巴细胞增殖均有促进作用。而绞股蓝多糖、薯蓣多糖、金樱子多糖、楮实多糖、竹根假万寿竹多糖能诱导RAW264.7细胞TNF-α表达上调。结论：九种黔产植物多糖在促淋巴细胞增殖和巨噬细胞活化中表现出明显差异,其中绞股蓝多糖有较好的免疫增强活性。     

瑶药枸骨叶不同溶剂组分体外抑菌活性比较

采用微量稀释法测定瑶药枸骨叶5种不同溶剂组分的最小抑菌浓度(MIC)，探讨枸骨叶不同溶剂提取物的体外抑菌活性．结果表明枸骨叶中的3种溶剂组分均显示一定的抑菌效果，其乙酸乙酯萃取物对金黄色葡萄球菌和大肠埃希氏菌的抑菌效果最为显，醇提物和正丁醇萃取物对金黄色葡萄球菌也显示较好的抑菌效果。     

白杨素对小鼠T细胞体外活化、增殖和细胞周期的影响

目的:研究白杨素(CR)对刀豆蛋白A(ConA)刺激的小鼠T细胞体外活化、增殖和细胞周期的影响,初步探讨其免疫调节作用机制。方法:利用荧光标记的单克隆抗体染色结合流式细胞术,检测小鼠活化T细胞CD69的表达情况;用羧基荧光素已酰已酸(CFDA-SE)染色结合流式细胞术,分析T细胞增殖相关指数;用碘化丙锭(PI)染色分析细胞周期分布。结果:加入终浓度为5、25、50μmol/L的CR后,活化T细胞CD69的表达率由ConA对照组的(85.12±1.07)%,分别降低为(78.42±1.82)%、(66.24±1.43)%和(53.09±1.77)%;72 h的T细胞增殖指数由ConA对照组的2.29±0.14分别降至1.91±0.02、1.47±0.03和1.14±0.01;对细胞周期的影响表现为:随着CR浓度升高,处于G0/G1期的细胞增加,S期细胞减少,25μmol/L时,G2/M期细胞也减少。结论:CR能有效抑制小鼠T细胞的体外活化和增殖,阻滞活化细胞从G1期进入S期,是一种潜在的免疫抑制剂。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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