Biochemical polymorphism and the 2,3-diphosphoglycerate in the sheep red blood cells

Summary There was no significant difference in the level of 2,3-DPG in the red blood cells of sheep of different haemoglobin types (Hb A and Hb B) or potassium types (HK and LK). However, low glutathione (GSH^L) sheep had significantly higher (p<0.01) level of 2,3-DPG in their red blood cells than high glutathione (GSH^H) sheep. There was also significant effect of interactions between glutathione, haemoglobin and potassium types (p<0.05) and glutathione and haemoglobin types (p<0.01) on red cell 2,3-DPG levels.     

The effect of anaerobic incubation upon 2,3-diphosphoglycerate synthesis in vitro

Zusammenfassung In Erythrozyten ist die Synthese von 2, 3-Diphosphoglycerat aus Fructose-1, 6-diphosphat in anaerobem Milieu um 18% höher als unter aeroben-Bedingungen. Das anaerobe Milieu steigert die Aktivität der Glyceraldehyd-3-phosphat-dehydrogenase um 300%.     

Erythrocyte chimaerism inW-series anaemic mice after allogeneic splenic transplants to the renal cortex

Résumé Une portion d'une rate allogénique normale été transplantée sur le rein de souris anémiques, génotypeW ^v W ^v , immunosuppressent avec le sérum antilymphocyte; 64% ont guéri. Les cellules hématopoïétiques émigrent hors de la greffe dans la moelle osseuse, s'y fixent, proliférent et remplacent les cellules défectueuses de l'hôte.     

Stimulative effect of 2,3-diphosphoglycerate on methemoglobin reduction by ascorbic acid

Summary The reduction of methemoglobin by ascorbic acid was accelerated by the presence of 2,3-diphosphoglycerate     

The relative effect of 2,3-diphosphoglycerate on the oxygen affinity of fetal and adult hemoglobin in whole blood

Riassunto Experimenti condotti sul sangue intero di individui neonati e adulti hanno dimostrato che l'affinità del sangue per l'ossigeno (P 50) dipende dalla concentrazione intraeritrocitaria di 2,3-difosfoglicerato (DPG) e dalle proporzioni relative di emoglobina adulta (HbA) e fetale (HbF). I risultati ottenuti indicano che nel sangue in toto l'effetto del DPG sulla P50 della HbF è circa il 40% di quello sulla P50 della HbA.     

Erythrocyte membrane lipid peroxidation in iron deficiency anemia

Erythrocytes from normal subjects and from cases of iron deficiency anemia were exposed to hydrogen peroxide and the extent of membrane lipid peroxidation studied. Significantly less peroxidation was observed in intact anemic erythrocytes compared to normal. However, when isolated membrane lipids were subjected to peroxidation, there was no significant difference between the two groups. It is unlikely that lipid peroxidation per se plays a major role in the reported decrease in red cell life-span in iron deficiency.     

Erythrocyte NADH-methemoglobin reductase activity in experimental riboflavin deficiency

Zusammenfassung Bei Ratten mit Riboflavin-Mangel wurde eine normale Aktivität der NADH-abhängigen Methämoglobinreduktase festgestellt.     

Erythrocyte membrane lipid peroxidation in iron deficiency anemia

Summary Erythrocytes from normal subjects and from cases of iron deficiency anemia were exposed to hydrogen peroxide and the extent of membrane lipid peroxidation studied. Significantly less peroxidation was observed in intact anemic erythrocytes compared to normal. However, when isolated membrane lipids were subjected to peroxidation, there was no significant difference between the two groups. It is unlikely that lipid peroxidation per se plays a major role in the reported decrease in red cell life-span in iron deficiency. *** DIRECT SUPPORT *** A2025146 00003     

Erythrocyte magnesium and HLA groups]

Red blood cell Magnesium levels were determined in 351 unrelated male subjects with known HLA groups. Significantly lower values are observed among subjects carrying BW 35 allele. Furthermore, significant variations are also noted according to the constitution of HLA-B groups with respect to BW 4 and BW 6 public specificities. This observation brings to light other variations among the subjects carrying B 8 allele.     

Erythrocyte cation concentrations and changes in dietary electrolyte intake

Résumé Les taux de Na, K et Mg dans les érythrocytes de 4 hommes sont restés constants lorsque les sujects prenaient des diètes avec des quantités très différentes de cas cathions. Ce résultat indique que dans l'érythrocyte les concentrations de ces ions ne sont pas modifiées par des variations très marquées de la quantité des électrolytes ingestés.

---

---

This work was supported in part by Part I Research Funds from the Veterans Administration. We thank Mrs.S. Hemphill, R. D., for her assistance in the preparation of the experimental diets. The technical assistance of Mrs.J. Verna is gratefully appreciated.     

Erythrocyte catalase in liver cirrhosis and in experimental liver injury

Résumé Nous avons trouvé que l'activité de la catalase des erythrocytes chez les cirrhotiques et chez les chiens intoxiqués par CCl₄ est considérablement diminuée, tandis que le numéro globulaire et le taux d'hémoglobine ne représentent que des changements très peu signicatifs.     

Erythrocyte glucose-6-phosphate dehydrogenase deficiency and thalassemia

Résumé L'activité de la glucose-6-phosphate déhydrogènase était normale dans les érythrocytes obtenus de sujets atteints de Thalassémie (majeure ou mineure) d'Hémoglobine E - Thalassémie et dans des érythrocytes contenant une variété d'hémoglobines anormales. Chez trois des six sujets atteints d'Hémoglobine H - Thalassémie, la glucose-6-phosphate déhydrogènase était absente.     

Erythrocyte catechol-0-methyltransferase and plasma dopamine-beta-hydroxylase in human umbilical cord blood

Plasma dopamine-beta-hydroxylase enzymatic activity and immunoreactive protein levels in human umbilical cord blood are only about 2% as great as values in the blood of older subjects. Erythrocyte catechol-0-methyltransferase activity levels in umbilical cord blood are very similar to those in the blood of adult subjects.