共查询到20条相似文献,搜索用时 15 毫秒
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Cheng JC Yeh YJ Tseng CP Hsu SD Chang YL Sakamoto N Huang HD 《Cellular and molecular life sciences : CMLS》2012,69(15):2621-2633
The non-coding microRNA (miRNA) is involved in the regulation of hepatitis C virus (HCV) infection and offers an alternative target for developing anti-HCV agent. In this study, we aim to identify novel cellular miRNAs that directly target the HCV genome with anti-HCV therapeutic potential. Bioinformatic analyses were performed to unveil liver-abundant miRNAs with predicted target sequences on HCV genome. Various cell-based systems confirmed that let-7b plays a negative role in HCV expression. In particular, let-7b suppressed HCV replicon activity and down-regulated HCV accumulation leading to reduced infectivity of HCVcc. Mutational analysis identified let-7b binding sites at the coding sequences of NS5B and 5'-UTR of HCV genome that were conserved among various HCV genotypes. We further demonstrated that the underlying mechanism for let-7b-mediated suppression of HCV RNA accumulation was not dependent on inhibition of HCV translation. Let-7b and IFNα-2a also elicited a synergistic inhibitory effect on HCV infection. Together, let-7b represents a novel cellular miRNA that targets the HCV genome and elicits anti-HCV activity. This study thereby sheds new insight into understanding the role of host miRNAs in HCV pathogenesis and to developing a potential anti-HCV therapeutic strategy. 相似文献
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The hepatitis C viruses (HCVs) are a group of small enveloped RNA viruses that have been viewed as a leading cause of chronic
hepatitis in humans. Infections by HCV represent a serious global health problem, because millions of people worldwide are
infected and no efficient treatment is available at the present time. Since HCV was identified in 1989, considerable effort
has been devoted to the discovery and development of novel molecules to treat HCV-related diseases. One of the approaches
is the development of novel inhibitors that interrupt the normal functions of HCV NS5B, an RNA-dependent RNA polymerase essential
to HCV replication. This review summarizes recent advances in the biochemical and structural understanding of HCV NS5B polymerase
as well as in the development of antiviral agents targeting this important enzyme.
Received 19 March 2002; received after revision 23 April 2002; accepted 23 April 2002 相似文献
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Structural aspects of rabies virus replication 总被引:2,自引:0,他引:2
Albertini AA Schoehn G Weissenhorn W Ruigrok RW 《Cellular and molecular life sciences : CMLS》2008,65(2):282-294
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O Hantz L Vitvitski C Pichoud C Trepo 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1979,289(16):1263-1266
Hepatitis B virus-like particles (including DANE particles) with DNA polymerase activity but negative for HBs Ag have been identified in NON-A, NON-B hepatitis sera positive for HC Ag. Although specifically associated with the particles, HC Ag is not a surface antigen of the hepatitis C virus identified here for the first time. The relationship of this agent with HBV seems obvious, and deserves further study. 相似文献
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Pauline Ferraris Elodie Beaumont Rustem Uzbekov Denys Brand Julien Gaillard Emmanuelle Blanchard Philippe Roingeard 《Cellular and molecular life sciences : CMLS》2013,70(7):1297-1306
Like most positive-strand RNA viruses, hepatitis C virus (HCV) forms a membrane-associated replication complex consisting of replicating RNA, viral and host proteins anchored to altered cell membranes. We used a combination of qualitative and quantitative electron microscopy (EM), immuno-EM, and the 3D reconstruction of serial EM sections to analyze the host cell membrane alterations induced by HCV. Three different types of membrane alteration were observed: vesicles in clusters (ViCs), contiguous vesicles (CVs), and double-membrane vesicles (DMVs). The main ultrastructural change observed early in infection was the formation of a network of CVs surrounding the lipid droplets. Later stages in the infectious cycle were characterized by a large increase in the number of DMVs, which may be derived from the CVs. These DMVs are thought to constitute the membranous structures harboring the viral replication complexes in which viral replication is firmly and permanently established and to protect the virus against double-stranded RNA-triggered host antiviral responses. 相似文献
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Riassunto Nell'epatite virale umana acuta l'attività fosfoglicomutasica del siero è notevolemente aumentata rispetto ai valori normali.L'aggiunta di omogenato bollito di fegato di topo produce ulteriore notevole incremento dell'attività fosfoglicomutasica del siero di soggetti affetti da epatite epidemica; non influenza tale attività nel siero di soggetti normali. 相似文献
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C Blaineau S Gisselbrecht M A Hurot F Pozo 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,284(23):2427-2430
The use of our mink cell line maintained in vitro infected with the murine xenotropic AT 124 virus, and that of a (W/Fu x bn) f1 rat anti-124 serum allow us to define a new cell surface antigen specific of murine xenotropic type C viruses. 相似文献
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The metabolism, pharmacokinetics and mechanisms of antiviral activity of ribavirin against hepatitis C virus 总被引:2,自引:0,他引:2
Ribavirin, a broad spectrum antiviral agent, in conjunction with interferon forms the current standard of treatment for hepatitis
C virus (HCV) infection in humans. While ribavirin alone fails to induce a significant antiviral response, in combination
with interferon, ribavirin dramatically improves the long-term outcome of therapy. The predominant mechanism(s) of ribavirin
action against HCV, are yet to be established. In this review, we examine the current status of our understanding of the metabolism,
pharmacokinetics and mechanisms of the antiviral activity of ribavirin against HCV, all of which are central to the rational
identification of improved treatment protocols.
Received 30 September 2005; received after revision 20 November 2005; accepted 7 December 2005 相似文献
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C Lavialle A G Morris H G Suárez R Cassingena 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1975,281(16):1203-1206
The production of virions by Chinese hamster kidney cells infected with SV 40 DNA is increased 10 to 100 fold by mitomycin C treatment. This observation has been confirmed for different cell clones. The optimum concentration of mitomycin C has been determined. 相似文献
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Résumé L'acide oxamyl-hydroxamique, l'acide salicylhydroxamique et l'acétoxyoxamide inhibent la reproduction du bactériophage T4 dans l'Escherichia coli B. L'activité de chacun de ces composés était plus grande que celle de l'hydroxyurée dans le même système d'essai. De ce fait, une évaluation plus poussée de ces 3 agents ainsi que d'autres acides hydroxamiques dans une série de systèmes d'essai sur les virus paraît clairement indiquée. 相似文献
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Marion Depla Rustem Uzbekov Christophe Hourioux Emmanuelle Blanchard Amélie Le Gouge Ludovic Gillet Philippe Roingeard 《Cellular and molecular life sciences : CMLS》2010,67(18):3151-3161
Hepatitis C virus (HCV) release is linked to the formation of lipid droplet (LD) clusters in the perinuclear area of infected
cells, induced by the core protein. We used electron microscopy (EM) to monitor and compare the number and size of LD in cells
producing the mature and immature forms of the HCV core protein, and 3D EM to reconstruct whole cells producing the mature
core protein. Only the mature protein coated the LD and induced their clustering and emergence from endoplasmic reticulum
membranes enriched in this protein. We found no particular association between LD clusters and the centrosome in reconstructed
cells. The LD clustering induced by the mature core protein was associated with an increase in LD synthesis potentially due,
at least in part, to the ability of this protein to coat the LD. These observations provide useful information for further
studies of the mechanisms involved in HCV-induced steatosis. 相似文献
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Riassunto Le modalità di secrezione dei granuli contenenti Calcitonina da parte delle cellule C sono state studiate in tiroidi di cane in condizioni normali e in coltura organotipica con alto tenore di calcio. È stata notata la presenza di numerosi microtubuli alla periferia delle cellule e alcune immagini suggeriscono un attacco dei microtubuli ai granuli secretori. Viene prospettato che i microtubuli abbiano importanza nel meccanismo di secrezione delle cellule C e forse delle cellule della serie APUD in generale, e che questo possa essere del tipo «emiocitosi». 相似文献