共查询到20条相似文献,搜索用时 93 毫秒
1.
Summary The effects of 4-benzylhydroxy-3,5-diiodobenzoic acid on isolated mitochondria ofWalker carcinosarcoma 256 and liver tissue of the same animals were compared. It was shown that, by the same concentrations of the uncoupling halo-phenol, the oxidative phosphorylation of tumor mitochondria was impaired in a higher degree than that of liver mitochondria. 相似文献
2.
Adenylate kinase activity of intact mitochondria is strongly inhibited by Ap5A, i.e. p1,p5-Di (adenosine-5') pentaphosphate, whereas oxidative phosphorylation is not affected. Therefore, Ap5A is a useful tool to distinguish between oxidative and non oxidative ATP generating reactions. 相似文献
3.
Direct evidence is provided for the transmembrane permeation of xanthomegnin across phospholipid bilayer membranes using ascorbate-loaded liposomes. This process may be associated with an uncoupling effect on the oxidative phosphorylation of mitochondria. 相似文献
4.
Summary Direct evidence is provided for the transmembrane permeation of xanthomegnin across phospholipid bilayer mebranes using ascorbate-loaded liposomes. This process may be associated with an uncoupling effect on the oxidative phosphorylation of mitochondria. 相似文献
5.
G. Laudahn 《Cellular and molecular life sciences : CMLS》1959,15(2):71-71
Summary The ability of isolated mitochondria in coupling phosphorylation to oxidation depends on sufficient concentrations of intramitochondrially
bound adenine nucleotides (especially ATP), compounds of oxidative metabolism (pyruvic and lactic acid) and iron ions. Continuous
loss of these components by ageing causes at first a parallel lowering of phosphate uptake and does not affect respiration.
Later on, a decrease of oxidative ability begins when the loss of intramitochondrial components reaches a limiting concentration,
and now mitochondria show more and more swelling and structural damages.
相似文献
6.
Summary The action on mitochondria of 3 peptide antibiotics, hypelcin-A, hypelcin-B, and alamethicin, was examined. The results showed that they are unique uncouplers of oxidative phosphorylation, with the same mechanism of action. 相似文献
7.
G. Laudahn 《Cellular and molecular life sciences : CMLS》1961,17(9):415-416
Summary Addition of Persantin to respiring beef heart mitochondria leads to a considerable increase of the P/O-ratios in the presence of various substrates. Apparently Persantin is capable of bringing about a tighter coupling of oxidation and phosphorylation. 相似文献
8.
Effect of albumin on uncoupling of oxidative phosphorylation by chinoform in rat liver mitochondria.
Addition of serum albumin diminished the uncoupling effect of chinoform on oxidative phosphorylation in rat liver mitochondria. Upon increasing the concentration of magnesium ions in the medium, the action of serum albumin was diminished. These results indicate that serum albumin combines with chinoform in competition with magnesium ions. 相似文献
9.
Hébert Chatelain E Dupuy JW Letellier T Dachary-Prigent J 《Cellular and molecular life sciences : CMLS》2011,68(15):2603-2613
Given the presence of Src and PTP1B within rat brain mitochondria, we have investigated whether PTP1B regulates Src activity
in mitochondria as in the cytosol. Results showed that Src was stimulated by in vitro addition of ATP to mitochondria, and
this stimulation was reversed by a membrane-permeable allosteric inhibitor of PTP1B and by a potent selective Src inhibitor.
They also indicated a direct action of PTP1B on phosphorylated tyrosine 527 residue of Src, thus implicating a role for PTP1B
in the modulation of Src activity in mitochondria. Putative Src and PTP1B substrates were identified by liquid chromatography
tandem mass spectrometry and two-dimensional blue native/SDS-PAGE. Both inhibitors inhibited ADP-stimulated respirations concurrently
with Src activation and complex IV activation by ATP, while having no effect or increasing the activity of the other complexes.
Our analysis emphasizes the regulatory function of Src and its modulation by PTP1B on oxidative phosphorylation in mitochondria. 相似文献
10.
P. Divakaran 《Cellular and molecular life sciences : CMLS》1978,34(12):1540-1541
Summary 2 dietary fats, namely, hydrogenated coconut oil and safflower seed oil were fed at 20% levels to weanling male albino rats for a period of 2 months after which the animals were sacrificed and oxidative phosphorylation measured in liver mitochondria. This ratio was more in the unsaturated-fat-fed group of rats compared to the saturated-fed ones for glutamate and malate; in the case of succinate no such change was noticed. 相似文献
11.
Cancer cell metabolism is characterized by limited oxidative phosphorylation in order to minimize oxidative stress. We have
previously shown that the flavonoid flavone in HT-29 colon cancer cells increases the uptake of pyruvate or lactate into mitochondria,
which is followed by an increase in O2−.. production that finally leads to apoptosis. Similarly, a supply of palmitoylcarnitine in combination with carnitine induces
apoptosis in HT-29 cells by increasing the mitochondrial respiration rate. Here we show that flavone-induced apoptosis is
increased more than twofold in the presence of palmitoylcarnitine due to increased mitochondrial fatty acid transport and
the subsequent metabolic generation of O2−. in mitochondria is the initiating factor for the execution of apoptosis.
Received 12 August 2005; received after revision 12 October 2005; accepted 14 October 2005 相似文献
12.
Lawrence R. Gray Sean C. Tompkins Eric B. Taylor 《Cellular and molecular life sciences : CMLS》2014,71(14):2577-2604
Pyruvate is a keystone molecule critical for numerous aspects of eukaryotic and human metabolism. Pyruvate is the end-product of glycolysis, is derived from additional sources in the cellular cytoplasm, and is ultimately destined for transport into mitochondria as a master fuel input undergirding citric acid cycle carbon flux. In mitochondria, pyruvate drives ATP production by oxidative phosphorylation and multiple biosynthetic pathways intersecting the citric acid cycle. Mitochondrial pyruvate metabolism is regulated by many enzymes, including the recently discovered mitochondria pyruvate carrier, pyruvate dehydrogenase, and pyruvate carboxylase, to modulate overall pyruvate carbon flux. Mutations in any of the genes encoding for proteins regulating pyruvate metabolism may lead to disease. Numerous cases have been described. Aberrant pyruvate metabolism plays an especially prominent role in cancer, heart failure, and neurodegeneration. Because most major diseases involve aberrant metabolism, understanding and exploiting pyruvate carbon flux may yield novel treatments that enhance human health. 相似文献
13.
Lionetti L Crescenzo R Mollica MP Tasso R Barletta A Liverini G Iossa S 《Cellular and molecular life sciences : CMLS》2004,61(11):1366-1371
This study was designed to examine the effect of youth-adulthood transition on hepatic mitochondrial energy efficiency. The changes in basal and palmitate-induced proton leak, which contribute to mitochondrial efficiency, were evaluated in mitochondria isolated from the liver of young and adult rats. Alterations in mitochondrial cytochrome oxidase and aconitase specific activities, and in adenine nucleotide translocator content were also assessed. There was no difference in basal proton leak or thermodynamic coupling and efficiency of oxidative phosphorylation in liver mitochondria between the two rat groups. On the other hand, palmitate-induced proton leak increased significantly in adult rats. The function of this uncoupling could be avoidance of elevated formation of reactive oxygen species, which are known to accelerate ageing.Received 17 February 2004; received after revision 30 March 2004; accepted 1 April 2004 相似文献
14.
Summary Adenylate kinase activity of intact mitochondria is strongly inhibited by Ap5A, i.e.p
1,p
5-Di (adenosine-5-) pentaphosphate, whereas oxidative phosphorylation is not affected. Therefore, Ap5A is a useful tool to distinguish between oxidative and non oxidative ATP generating reactions.Acknowledgment. The generous support of Prof. Dr.Walther Lamprecht is gratefully acknowledged. J. L. thanks the Stipendienfonds des Verbandes der Chemische Industrie for a scholarship. 相似文献
15.
Identification of tyrosine-phosphorylated proteins of the mitochondrial oxidative phosphorylation machinery 总被引:1,自引:1,他引:0
Augereau O Claverol S Boudes N Basurko MJ Bonneu M Rossignol R Mazat JP Letellier T Dachary-Prigent J 《Cellular and molecular life sciences : CMLS》2005,62(13):1478-1488
The role of some serine/threonine kinases in the regulation of mitochondrial physiology is now well established, but little is known about mitochondrial tyrosine kinases. We showed that tyrosine phosphorylation of rat brain mitochondrial proteins was increased by in vitro addition of ATP and H2O2, and also during in situ ATP production at state 3, and maximal reactive oxygen species production. The Src kinase inhibitor PP2 decreased tyrosine phosphorylation and respiratory rates at state 3. We found that the 39-kDa subunit of complex I was tyrosine phosphorylated, and we identified putative tyrosine-phosphorylated subunits for the other complexes. We also have strong evidence that the FoF1-ATP synthase α chain is probably tyrosine-phosphorylated, but demonstrated that the β chain is not. The tyrosine phosphatase PTP 1B was found in brain but not in muscle, heart or liver mitochondria. Our results suggest that tyrosine kinases and phosphatases are involved in the regulation of oxidative phosphorylation.Received 7 January 2005; received after revision 19 April 2005; accepted 22 April 2005 相似文献
16.
Silvia Ravera Marina Podestà Federica Sabatini Chiara Fresia Marta Columbaro Silvia Bruno Ezio Fulcheri Luca Antonio Ramenghi Francesco Frassoni 《Cellular and molecular life sciences : CMLS》2018,75(5):889-903
We evaluated the energy metabolism of human mesenchymal stem cells (MSC) isolated from umbilical cord (UC) of preterm (< 37 weeks of gestational age) and term (≥ 37 weeks of gestational age) newborns, using MSC from adult bone marrow as control. A metabolic switch has been observed around the 34th week of gestational age from a prevalently anaerobic glycolysis to the oxidative phosphorylation. This metabolic change is associated with the organization of mitochondria reticulum: preterm MSCs presented a scarcely organized mitochondrial reticulum and low expression of proteins involved in the mitochondrial fission/fusion, compared to term MSCs. These changes seem governed by the expression of CLUH, a cytosolic messenger RNA-binding protein involved in the mitochondria biogenesis and distribution inside the cell; in fact, CLUH silencing in term MSC determined a metabolic fingerprint similar to that of preterm MSC. Our study discloses novel information on the production of energy and mitochondrial organization and function, during the passage from fetal to adult life, providing useful information for the management of preterm birth. 相似文献
17.
M Neuburger R Douce 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,285(8):881-884
Spinach leaf mitochondria (C3 plant) were capable of oxidizing Glycine. This oxidation was linked to the mitochondrial electron transport chain, was coupled to three phosphorylation sites and was sensitive to electron transport inhibitors. In marked contrast however, neither mitochondria from dark grown plants nor mitochondria from C4 leaf plants were capable of oxidizing this amino acid. 相似文献
18.
19.
In eukaryotes, cellular energy in the form of ATP is produced in the cytosol via glycolysis or in the mitochondria via oxidative
phosphorylation and, in photosynthetic organisms, in the chloroplast via photophosphorylation. Transport of adenine nucleotides
among cell compartments is essential and is performed mainly by members of the mitochondrial carrier family, among which the
ADP/ATP carriers are the best known. This work reviews the carriers that transport adenine nucleotides into the organelles
of eukaryotic cells together with their possible functions. We focus on novel mechanisms of adenine nucleotide transport,
including mitochondrial carriers found in organelles such as peroxisomes, plastids, or endoplasmic reticulum and also mitochondrial
carriers found in the mitochondrial remnants of many eukaryotic parasites of interest. The extensive repertoire of adenine
nucleotide carriers highlights an amazing variety of new possible functions of adenine nucleotide transport across eukaryotic
organelles. 相似文献
20.
Parihar MS Parihar A Fujita M Hashimoto M Ghafourifar P 《Cellular and molecular life sciences : CMLS》2008,65(7-8):1272-1284
α-Synuclein is a neuron-specific protein that contributes to the pathology of Parkinson’s disease via mitochondria-related mechanisms. The present study investigated possible interaction of α-synuclein with mitochondria and
consequences of such interaction. Using SHSY cells overexpressing α-synuclein A53T mutant or wild-type, as well as isolated
rat brain mitochondria, the present study shows that α-synuclein localizes at the mitochondrial membrane. In both SHSY cells
and isolated mitochondria, interaction of α-synuclein with mitochondria causes release of cytochrome c, increase of mitochondrial calcium and nitric oxide, and oxidative modification of mitochondrial components. These findings
suggest a pivotal role for mitochondria in oxidative stress and apoptosis induced by α-synuclein.
Received 27 December 2007; received after revision 7 February 2008; accepted 8 February 2008 相似文献