一株橙色黏球菌的筛选及初步鉴定

从土样中通过菌体诱导和纤维素诱导法来富集和选择性分离黏细菌,将初筛菌株进行抗菌谱测定,挑选抑菌活性强的一株(编号为hg59)进行系统分类鉴定.结果表明:该菌株革兰氏染色阴性,营养细胞呈杆状,两头稍尖,大小(5～8)μm×(0.3～0.6)μm,黏孢子为卵圆形或椭圆形,菌落生长初期为淡黄色,渐变成橙黄色,薄而向边缘扩展,有滑动痕迹,对G+,G-菌均具有抑菌活性,其中对枯草芽孢杆菌和醋酸杆菌抑菌效果明显,对米曲霉则较差.经形态观察、生理生化测定和16SrDNA序列分析,初步鉴定为橙色黏球菌(Myxococcus fulvus strain).     

南美白对虾虾苗淡化期间发光病病原研究

2002年,浙江某南美白对虾(Penaeusvannamei)苗种场在虾苗淡化过程中发生了发光病.通过对典型发光病虾的镜检、细菌分离,结果从濒死病虾体内分离到4株细菌,其中菌株Pv-1在TCBS和TSA培养基上均能发光,用此菌株浸泡感染健康南美白对虾,可复制出发光病虾,且有较高的死亡率。对Pv-1进行了培养特性和生化特性鉴定,确定Pv-1为革兰氏阴性菌,菌体呈直杆状,大小为1.5μm×0.8μm,极生单鞭毛运动,氧化酶阳性,发酵葡萄糖,对弧菌抑制剂0 129(150μg)敏感,经鉴定为哈维氏弧菌(Vibrioharveyi).由此表明哈维氏弧菌为此次南美白对虾虾苗淡化期间发光病的病原.     

芯片表面微翅片强化FC-72流动沸腾传热

为采用高效传热技术来提高芯片的冷却效率 ,保证芯片的正常工作 ,研究了不带电介质FC 72在微翅片尺寸 (厚度×翅片高 )分别为 5 0μm× 190μm ,5 0μm× 2 5 0μm ,10 0μm× 15 0μm和 10 0μm× 30 0μm的 4种强化面芯片表面的流动与沸腾传热性能 ,探讨了FC 72过冷度对沸腾传热的影响 ,并与光滑面芯片的沸腾传热性能进行了对比 .实验结果表明 ,随着FC 72过冷度的增大 ,所有实验芯片的临界热流密度增大 .强化面芯片的临界热流密度所对应的壁温低于 85℃ ,而光滑面芯片的临界热流密度所对应的壁温高于85℃ .在相同过冷度下 ,强化面芯片的最大热流密度是光滑面芯片的 7～ 9倍 ,表明微翅片结构能显著地强化不带电介质FC 72的沸腾传热     

一株聚β-羟基丁酸高产菌株的分离与研究--Ⅱ.出发菌株的筛选鉴定及合成PHB条件的研究

从城市生活污水中分离筛选到一菌株 ,具如下主要特征 :杆菌 ,(0 .85～ 1 .2 )× (2 .0～ 5.0 )μm;革兰氏染色阴性 ;具衣鞘 ,细胞包在鞘里 ,丝状体很长 ,但无分枝 ;亚端生鞭毛 ;胞内具 PHB颗粒和异染颗粒 ;液化明胶微弱 ;菌落为光滑型和丝状两种 .经鉴定该菌株为浮游球衣细菌(Sphaerotilus natans) .该菌在 2 8℃条件下生长良好 ,细菌细胞大 ,细胞内可积累大量的 PHB.从对数期至稳定期 ,该菌株积累的 PHB量可达细胞干重的 1 9.8%     

桂花叶尖皱缩病病原的鉴定

对桂花叶尖皱缩病病原作研究后初步鉴定系胶胞炭疽病菌 (Colletotrichum gloeosporioides (Penz)Sacc .)侵染所致 .分生孢子无色、单孢圆柱形 ,端部钝 ,8.80～ 19.2 μm× 4.48～ 5 .44 μm ,有油滴数个 ;附着孢褐色、形状简单、大多椭圆形 ,大小 4.2～ 6 .6 μm× 5 .3～ 8.5 μm .     

芽孢杆菌属一新种的鉴定

从萧县盐碱地土样中分离到一株耐盐耐碱芽孢杆菌,其可在NaCl浓度为5%～10%的培养基中生长良好,耐碱性可达pH值为11.该菌好氧,菌体杆状,具芽孢,芽孢椭圆形,膨大,端生,菌体常连成线丝状,细胞大小为(0.6～0.8)μm×(2.5～4)μm,菌落白色大型,中有一色浅圈,边缘向周围延展成波纹状,菌落中部较厚,不透明,边缘延展部分较薄,无色透明.该菌接触酶阳性,水解淀粉,硝酸盐还原,L—阿拉伯糖和D—木糖不产酸,不利用柠檬酸盐,V·P反应阴性,在V·P肉汤中生长后pH为6.5～7,形成吲哚、溶菌酶(0.001%)不敏感.根据这些特征,该菌应归芽孢杆菌属(Bacillus),同时又不同于该属的其它已知菌株,因此将它定为新种,命名为耐盐耐碱芽孢杆菌(Bacillushaloalkaloresistatsp·nov·).     

牛蛙"白内障"病病原的初步研究

从患"白内障"病的牛蛙肝脏、心脏、眼睛等器官中分离到A 1 L、B 4 H、B 2 E3株细菌.各以108CFU/mL的浓度进行肌肉注射健康蛙,剂量为0.2mL.发现B 2 E分离菌可使实验蛙100%产生与自然发病相似的"白内障"症状,死亡率达70%,可确认该菌为"白内障"病的致病菌.该分离菌大小为(0.3～0.5)μm×(0.6～0.8)μm,短杆状,极生单鞭毛,革兰氏阴性,无芽孢,无荚膜,主要生理生化特性是葡萄糖产酸、产气;氧化酶、赖氨酸脱羧酶、精氨酸脱羧酶阳性;鸟氨酸脱羧酶阴性;能在TCBS培养基上生长、培养基呈绿色,但不能在6%NaCl蛋白胨水上生长.根据其主要生理生化特性,初步鉴定为气单胞菌(Aeromonassp.).药敏试验结果表明,该菌对链霉素等敏感,对氨苄青霉素、青霉素G、先锋霉素V等不敏感.     

位山引黄灌区农田土壤磁化率与粒度特征及其相关性分析

以位山引黄灌区为研究区,测定了农田土壤剖面的磁化率和粒度,并分析了二者的相关关系.结果显示:位山引黄灌区农田土壤低频磁化率(xlf)的变化范围为35.37-97.20×10~(-8)m~3/kg,均值为62.16×10~(-8) m~3/kg;频率磁化率(xfd)的变化范围为0.65%-22.14%,平均值为7.23%.土壤中粘粒(4μm)含量变化范围为3.20%-27.16%,平均值为9.07%;粉砂(4-63μm)含量变化范围为35.82%-90.23%,平均值为68.94%;砂粒(63μm)含量变化范围为1.95%-58.52%,平均值为21.99%;中值粒径的变化范围为9.56-72.86μm,平均值为34.94μm.相关性分析结果表明:剖面0-20cm土壤xlf与粉砂(4-63μm)含量呈显著负相关,与砂粒(63μm)含量及中值粒径呈显著正相关,与粘粒(4μm)含量相关性较弱,土壤xfd与各粒度组分含量及中值粒径的相关性均不显著.剖面20-80cm土壤xlf与粉砂(4-63μm)含量呈显著负相关,与砂粒(63μm)含量呈显著正相关,与中值粒径的相关性较显著,与粘粒(4μm)含量相关性不显著;土壤xfd与粘粒(4μm)及粉砂(4-63μm)含量相关性较显著,与砂粒(63μm)含量及中值粒径的相关性均不显著.研究认为:土壤磁化率在0-20cm耕作层主要受到土壤母质以及灌溉、施肥等人类活动的影响,而在20-80cm主要受土壤母质差异的影响.     

木麻黄防护林基干林带更新改造与降风效能分析

针对木麻黄基干老林带,分别采取不同采伐面积进行更新改造.结果表明三种不同面积(20m×20m、20m×30m、30m×30m)采用木麻黄惠安1号优良无性系、挖大穴、下客土拌磷肥、雨天造林等配套技术更新改造效果较佳.6年生时平均树高已达7.9m,超过木麻黄老林带平均5.8m的高度.但新林带受风害程度随采伐更新面积增大而加剧.试验还表明,木麻黄新林带20m×30m处理,明显比其它3种处理降低风速提高0.9%～2.5%.老林带更新不仅成功,而且都具有较好的降风效能,值得推广应用.     

金鱼"突眼病"病原的初步研究

从患典型"突眼病"金鱼的眼球、肝脏中,分别分离出E-01和L-01菌,经人工感染试验确认E-01菌为该病的致病菌.该菌菌体大小为(1.0～1.5)μm×(1.5～2.3)μm,革兰氏阴性,无鞭毛;触酶阳性,氧化酶、硫化氢、吲哚、蔗糖、赖氨酸脱羧酶、鸟氨酸脱羧酶、精氨酸双水解酶等阴性.根据其生理生化特性确定为鲁氏不动杆菌(Acinebobacterlwoffi).药敏试验结果表明:其对先锋霉素V、卡那霉素、多粘菌素B、复方新诺明、庆大霉素、链霉素、新霉素等药物敏感.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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