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D. C. Viza 《Cellular and molecular life sciences : CMLS》1967,23(10):848-849
Résumé Les lymphocytes du sang périphérique d'un sujet ont été fractionnés. Trois fractions ont été obtenues. Chaque fraction a été cultivée avec les lymphocytes vivants d'un autre individu non apparenté au premier. Le pourcentage des cellules transformées a été évalué pour chaque culture. Seule la fraction ribosomale dans laquelle se trouvent également les membranes cellulaires est active et donne un pourcentage de transformations comparable à celui obtenu lorsqu'on met en culture dans les mêmes conditions les lymphocytes, entiers et tués par congélation-décongélation, qui ont servi pour la préparation des fractions.
Supported by a Grant from Lady Tata Foundation. 相似文献
Supported by a Grant from Lady Tata Foundation. 相似文献
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Summary The levels of membrane alkaline phosphatase have been measured on different lymphocyte fractions from human peripheral blood separated on bovine serum albumin discontinuous gradients. The peak in enzyme activity was observed in a non-T-, non-B-cell fraction, rich in null lymphocytes; the lowest values were found in the fraction with the highest proportion of T-cells. 相似文献
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G. P. Young M. B. Van der Weyden I. S. Rose F. J. Dudley 《Cellular and molecular life sciences : CMLS》1979,35(2):268-269
Summary The basis of peripheral blood lymphopenia observed in patients with chronic alcoholism and liver disease was investigated by examining the effect of sera of these patients on in vitro transformation of normal human peripheral blood lymphocytes. A positive correlation was demonstrated between the serum inhibition of phytohaemagglutinin- and pokeweed mitogen-induced transformation and the degree of lymphopenia. Thus serum factors may contribute to the observed lymphopenia by inhibiting lymphocyte production in vivo.This study was supported by grants from the National Health and Medical Research Council of Australia, Royal Australasian College of Physicians and Alfred Hospital Henry Laurie Scholarship Fund. 相似文献
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The basis of peripheral blood lymphopenia observed in patients with chronic alcoholism and liver disease was investigated by examining the effect of sera of these patients on in vitro transformation of normal human peripheral blood lymphocytes. A positive correlation was demonstrated between the serum inhibition of phytohaemagglutinin- and pokeweed mitogen-induced transformation and the degree of lymphopenia. Thus serum factors may contribute to the observed lymphopenia by inhibiting lymphocyte production in vivo. 相似文献
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The levels of membrane alkaline phosphatase have been measured on different lymphocyte fractions from human peripheral blood separated on bovine serum albumin discontinuous gradients. The peak in enzyme activity was observed in a non-T-, non-B-cell fraction, rich in "null" lymphocytes; the lowest values were found in the fraction with the highest proportion of T-cells. 相似文献
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A. L. Carmagnola M. Boccadoro A. Pileri 《Cellular and molecular life sciences : CMLS》1983,39(10):1137-1139
Summary Ten multiple myeloma (MM) and 5 monoclonal gammopathies of undertermined significance (MGUS) were studied. The mixed lymphocyte reaction (MLR) proliferative response was of the same order of magnitude in MM and in MGUS as in normal controls. Normal results were obtained when pathological lymphocytes were used as either responding or stimulating cells. The addition of monoclonal anti-Ia antibodies completely abrogates the proliferative response as in healthy individuals. These results suggest that the immunological mechanisms supposed to be important in MLR are functionally normal in MM and in MGUS, although both the B and T cell lineages are involved in monoclonal gammopathies.This work was supported by CNR-PPFF Controllo della crescita neoplastica No. 82.00387.96. We thank Regione Piemonte which purchasel most of the technical equipment. 相似文献
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R. Velasco Alonso J. Prieto Valtuena M. A. del Pozzo Perez M. I. Gonzales Guilabert L. Corporales Lopez 《Cellular and molecular life sciences : CMLS》1973,29(7):870-871
Resumen Se ha estudiado la relación existente entre la concentración de Ig. sericas y las poblaciones linfocitarias T y B en un grupo de sujetos ancianos, encontrandose un notable descenso de la transformación blasticá al tiempo que se incrementa el porcentage de linfocitos portadores de Ig. en su membrana. De nuestros resultados parece deducirse una relación entre el nivel de Ig. sericas y la población medulo-derivada. 相似文献
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Summary Normal human lymphocytes were cultured for 72 h with different doses of BrdU. The analysis of metaphases processed with the BrdU-Giemsa method shows that in leukocyte cultures 3 different lymphocyte populations coexist which are able to perform 1, 2 or 3 rounds of replication in vitro. Moreover, it was concluded that 5 g/ml is the minimal dose of BrdU inducing good differentiation in the areas of sister chromatid exchanges.This work was supported by grants from the International Atomic Energy Agency, the Consejo Nacional de Investigaciones Cientificas and the Comisión de Investigaciones Cientificas. 相似文献
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Mori M Benotmane MA Tirone I Hooghe-Peters EL Desaintes C 《Cellular and molecular life sciences : CMLS》2005,62(13):1489-1501
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U. Torelli St. Ferrari G. Torelli R. Cadossi S. Ferrari 《Cellular and molecular life sciences : CMLS》1976,32(3):285-287
Summary Ribonuclease activity directed against the synthetic duplex polyrC: polyrI was detected in nuclear extracts from both unstimulated and PHA-stimulated human lymphocytes. In the latter cells, the activity was about twice that of small lymphocytes. 相似文献
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M Vaiman C Renard M Ponceau J Lecointre P A Villiers 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1975,280(24):2809-2812
Immunizations between a pig bearing a recombined SL-A haplotype and related animals revealed serological defined antigens under the MLR region control. These antigens seemed to be carried by all types of lymphocytes so far studied, but with large quantitative differences. The blood nonadherent on nylon fiber lymphocytes carried very few antigens and platelets none at all. The data are compatible with the assumption that these newly discovered antigens are equivalent to the mouse Ia antigens. 相似文献
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Summary PHA-induced lymphocyte blastogenic response was remarkably suppressed by the simultaneous presence of PPD in cultures of lymphocytes derived from individuals sensitized to PPD, but not affected by the presence of PPD when the cultures contained lymphocytes from an individual not sensitized to the protein. This double stimulation blastogenesis study with PHA and a specific antigen is feasible as a simple and rapid test to measure cell-mediated immunity to the antigen.Supported by United States grant USPHS Grant AM 27384. 相似文献
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Ribonuclease activity directed against the synthetic duplex polyrC:polyI was detected in nuclear extracts from both unstimulated and PHA-stimulated human lymphocytes. In the latter cells, the activity was about twice that of small lymphocytes. 相似文献
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Hormones, thymus and lymphocyte functions 总被引:2,自引:0,他引:2
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Mouse serum factor depressing lymphocyte transformation 总被引:3,自引:0,他引:3
D S Nelson 《Experientia》1972,28(10):1227-1228