Further studies on hepatic acidic glycosaminoglycans in the Hurler syndrome.

Acid GAG were isolated from hepatic tissue from 3 patients with Hurler syndrome and 3 normal controls. Gross elevations in the uronic acid and hexosamine contents were found in Hurler livers compared with the normal ones. The total GAG concentration was significantly increased (about 25fold) in Hurler patients.     

Urinary acidic glycosaminoglycans in Werner's syndrome

Summary The composition of urinary acidic glycosaminoglycans (AGAG) in 4 patients with Werner's syndrome was determined by an enzymatic assay system using chondroitinases and hyaluronidase. In Werner's syndrome, the amount of hyaluronic acid and heparan sulfates in the total AGAG increases. A compositional change in the chondroitin sulfate isomers occurs. The change of urinary AGAG in Werner's syndrome appears to reflect age-related changes.The study was supported, in part, by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, the Life Science of the Institute of Physical and Chemical Research and the Adult Disease Clinic Memorial Foundation, Tokyo. Thanks are due to Dr Y. Yamada and Dr R. Abe for collecting specimens, and Miss R. Abe and Miss R. Yamaguchi for technical assistance.     

The acidic glycosaminoglycans of the synovial fluid in rheumatoid arthritis

Resumen Se estudiaron los glucosaminoglucanos del líquido sinovial de la rodilla de pacientes con artritis reumatoidea clásica. El nivel de glucosaminoglucanos totales desciende en la artritis un 55% comparado con los controles. Esto es debido principalmente a la disminución del ácido hialurónico (59%). Se confirmó la presencia de pequeñas cantidades de controidin-4-sulfato y se detectaron trazas de una sustancia similar al condroitin-6-sulfato.

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Member of the Carrera del Investigador Científico. Consejo Nacional de Investigaciones Científicas y Técnicas de la Repùblica Argentina.

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This work was partially supported by research grants from the CONICET, Republica Argentina.     

Changes in hepatic glycosaminoglycans following endotoxin administration

Summary Incorporation of [35]S-sulfate into hepatic glycosaminoglycans (GAGs) is affected by intravenous administration of endotoxin. There are significant increases in total labeled glycosaminoglycans and in the percentage of labeled dermatan sulfate 72 h post-endotoxin.     

The acidic glycosaminoglycans in the skin of athyroid rats: the effects ofl-tri-iodothyronine

Resumen Este estudio demuestra que en piel de ratas atiroideas, en comparación con las normales, el ácido hialurónico aumenta un 20% mientras que el dermatán sulfato disminuye un 28%, no encontrándose diferencias significativas en las demás fracciones de glycosaminoglycanos. Este efecto diferencial es similar al ya encontrado por nosotros en cartílago.

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This work was partially supported by the Consejo Nacional de Investigaciones Cientificas y Técnicas de la República argentina.

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Member of the Carrera del Investigador Científico del C.N.I.C.T. de la Argentina.     

The acidic glycosaminoglycans in gastric mucosa of athyroid rats: The effects of L-tri-iodothyronine

Summary Hyaluronic acid is increased about 12%, whereas dermatan sulfate is decreased about 34%, in gastric mucosa of athyroid rats when compared with controls. Administration of 2 doses of L-tri-iodothyronine reversed the effects of thyroidectomy.This work was partially supported by the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina.     

Further studies on the effect of 6-hydroxydopamine in retinal development

Summary Neonatal albino rats treated with 6-hydroxydopamine revealed depletion of rhodopsin and monoamine oxidase in their retinas.Acknowledgments. The author wishes to dedicate this paper to his family for their continuous support and to ProfessorsA. K. S. Ho andD. B. Meyer for their understanding and friendship.     

Further studies on the effect of 6-hydroxydopamine in retinal development.

Neonatal albino rats treated with 6-hydroxydopamine revealed depletion of rhodopsin and monoamine oxidase in their retinas.     

Further studies on gel filtration of urinary substances

Summary Urine specimens from healthy children were concentrated and filtered through Sephadex G-100 and G-25. The eluate was recovered in fractions of 5 ml each. Total hexoses, galactose, total proteins and total uronic acids were determined on each fraction. Total hexoses and galactose were eluted in 6 distinct peaks. The uronic acid containing GAG were eluted in 4 small peaks on G-25 and 1 peak on G-100. This peak is eluted always with high amounts of galactose containing glycoproteins.This work was partially supported by research grants from the CONICET, República Argentina.     

Further studies on the circadian rhythm in the proliferative activity of mouse intestinal epithelium

Zusammenfassung Im Tagesrhythmus der Proliferationstätigkeit von Darmepithelien wurde in drei verschiedenen Parametern um 02.00 Uhr ein Maximum gefunden. Mögliche Mechanismen werden erörtert.     

Further studies on gel filtration of urinary substances.

Urine specimens from healthy children were concentrated and filtered through Sephadex G-100 and G-25. The eluate was recovered in fractions of 5 ml each. Total hexoses, galactose, total proteins and total uronic acids were determined on each fraction. Total hexoses and galactose were eluted in 6 distinct peaks. The uronic acid containing GAG were eluted in 4 small peaks on G-25 and 1 peak on G-100. This peak is eluted always with high amounts of galactose containing glycoproteins.     

Effect of age on distribution of acidic glycosaminoglycans in normal human urine: Special reference to proportion of chondroitin sulfate A to the C-isomer

Résumé Le sulfate A de chondroitine est un acide glycosaminoglycanes principal chez les enfants normaux. Mais cette substance diminue avec l'age, tandis que le sulfate C de chondroïtine a une tendance à augmenter. Il en résulte une diminution, de l'action du sulfate A sur le sulfate C. La prépondérence du sulfate C de chondroïtine se manifeste après l'adolescence. Il augmente continuellement au cours de la vie.

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Acknowledgments. Grateful thanks are due to Prof.Y. Horiuchi for his interest and encouragement. We are indebted to Dr.T. Ishikawa and Dr.H. Ninomiya for preparing normal human urine. The present study was supported by a grant in aid for scientific research from the Education Ministry in Japan.     

Further studies on the influence of chloride on the renal tubular reabsorption of bicarbonate in the dog

Résumé L'intensité de la dépression qu'entraîne l'hyperchlorémie sur la réabsorption tubulaire du bicarbonate chez le chien paraît être essentiellement déterminée par le niveau initial de la réabsorption du bicarbonate.