Antischistosomal and some toxicological properties of a nitrodiphenylaminoisothiocyanate (C 9333-Go/CGP 4540).

A procedure to enhance the schistosomicidal effectiveness in vivo of an isothiocyanate derivative and some of its antischistosomal properties are reported. Determinations of the effects of this compound on tissue thiol levels and on highly sensitivity bacterial tester strains have indicated that its mutagenic potential is of a low order and that the latter is decreased further after reduction of the host's intestinal bacterial flora.     

Chemotherapeutic effects of 4-isothiocyanato-4'-nitrodiphenylamine (C9333-Go/CGP 4540) on infections with Nematospiroides dubius, Hymenolepis diminuta, Hymenolepis nana and Spirometra mansonoides.

4-Isothiocyanato-4'-nitrodiphenylamine possessed anthelmintic activity against Nippostrongylus dubius and Hymenolepis nana in mice and H. diminuta in rats as determined by both egg counts and recovery of parasites at autopsy. No activity was detected against the cestode. Spirometra mansonoides, in cats.     

Solid phase synthesis and some pharmacoogical properties of 4-Ser-8-Gln-Oxytocin (Glumitocin)

Zusammenfassung Eine neue Synthese des Peptidhormons Glumitocin wird beschrieben, in der die Trägermethode vonMerrifield benutzt wird. Einige pharmakologische Daten des Hormons werden mitgeteilt.

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This work was supported in part by a grant from the Medical Research Council of Canada, Banting Research Foundation, National Science Foundation Grant No. GB 4932X and a General Research Support Grant from the National Institutes of Health.     

Isolation and some properties of the heparin-neutralizing factor (PF4) released from human blood platelets

Zusammenfassung Der in Form eines hochmolekularen Komplexes von den Plättchen freigesetzte, heparinneutralisierende Faktor (PF 4) konnte reversibel in ein Träger-Material und ein aktives Protein (Molekulargewicht ca. 30000) aufgespalten werden.

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This work was supported by the Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung.     

The estrogenic and deciduogenic properties of some estra-1,3,5(10)triene-3,17β-diol derivatives

Zusammenfassung Die östrogene Aktivität von einigen substituierten Östrodiolderivaten wurde in Rattenvaginalabstrichen und Mäuseuterus-Wachstumversuchen nachgewiesen. Die Einführung von 17-Alkyl-, Alkenyloder Alkinylgruppen in die Östradiolgruppierung hatte einen signifikanten Effekt auf die östrogene Aktivität. Das 17-(2-Butinyl)-Derivat von Östradiol war eine der wirksamsten Substanzen in den Östrogen- und Deciduomaversuchen.     

Partial purification and some properties of a nucleoside phosphotransferase of chick embryos

Summary A nucleoside phosphotransferase purified about 40fold from chick embryos utilizes efficiently as phosphate donors deoxyribonucleoside and pyrimidine ribonucleoside monophosphates, whereas the pyrimidine deoxyribonucleoside appear to be the preferred acceptors of phosphate. The enzyme is very unstable to heat, dilution and dialysis. A marked enhancement in the stability is caused by nucleotides and it seems associated with the formation of an aggregated state of the protein.     

Morphological differentiation of neuroblastoma by 1 methyl cyclohexane carboxylic acid (CCA) and some C1 derivatives]

We describe the induction of neuroblastoma morphological differentiation by 1 methyl cyclohexane carboxylic acid (CCA) and by some C1 derivatives of CCA. This induction proceeds in a medium which allows, in the absence of inducer, a normal growth of neuroblastoma cells and contains 7.5% fetal Calf serum. In order to establish a clear-dose response relationship and to compare the relative potencies of different drugs, the morphological changes were assessed by examining "long neurite-bearing cells".     

C(14)-substituted corticosteroids. some D ring transformations in the compound S series

Zusammenfassung Eine Anzahl am D-Ring substituierter Derivate von Reichsteins Verbindung S wurde als Modellverbindungen in der Synthese neuer Corticosteroide hergestellt. Die beschriebenen Strukturtypen schliessen Dehydroverbindungen, Epoxyde, Halogenhydrine und ein Glykol ein.     

Hypolipidaemic properties of a new tetralin derivative (CIBA 13,437-Su)

Zusammenfassung 2-Methyl-2-[p-(1,2,3,4-tetrahydro-1-naphthyl)-phenoxy]-propion-säure (CIBA 13,437-Su)stellt ein oral aktives Tetralin-Derivat dar, welches ausgeprägte Blutlipid-senkende Eigenschaften besitzt. Die hypolipidaemische Wirkung wurde bei mehreren Spezies untersucht und umfasste sowohl Cholesterin wie Glyzeride.     

Effect of the scorpion,Heterometrus fulvipes (C. Koch), venom on some enzyme systems in rat (albino) tissues

Summary Sublethal doses ofHeterometrus fulvipes venom decreased NADP-specific isocitrate dehydrogenase and malate dehydrogenase activity levels and increased NADP-specific glucose-6-phosphate dehydrogenase and alkaline phosphatase activity levels during a 48-h-period.Acknowledgments. D. V. thanks the Council of Scientific and Industrial Research, New Delhi, for awarding a Senior Research fellowship.     

Common antigenic properties of a g-type (goose) and a c-type (duck) egg white lysozyme: Antibody responses in rabbits and mice

Embden goose (GEWL) and Barbary duck (DEWL) egg white lysozymes possess different amino acid sequences corresponding to the g-type and c-type, respectively. GEWL was shown to be a better immunogen than DEWL in both rabbits and mice. The antigenicity of the two lysozymes was tested using different technique (i.e. indirect ELISA, inhibition tests and immunoabsorption experiments). Injection of either GEWL or DEWL into rabbits and mice induced both specific antibodies and cross-reacting antibodies. Moreover, anti-GEWL antibodies, in contrast to anti-DEWL antibodies, did not cross-react with hen egg white lysozyme (HEWL), a c-type lysozyme. While the structure of GEWL was not modified after binding to plastic, DEWL was denaturated, but it did keep some native epitopes. It was concluded that g-type and c-type lysozymes, which have different amino acid sequences, exhibit strong common antigenic properties.     

Dystrophic mutation (dy2J) affecting regulation of lactate dehydrogenase (LDH) and pyruvate kinase (PK) in C57BL/6J mice

Summary The genotype difference (dystrophic vs nondystrophic) in the LDH isozymes is observed in kidney. These differences are evident only at birth and at early developmental stages (before the expression of dystrophic symptoms). The tissue specific genotype differences for PK are limited to the thigh muscle (M form) and heart (L form), after the onset of the condition. These differences may reflect the pleiotropic effect of the dy^2J locus during the temporal regulation of these and other enzymes implicated in muscular dystrophy (MD).This research was financed by a Natural Sciences and Engineering Research Council Canada grant to S.M.S.     

Effect of organophosphate pesticide Sumithion (Fenitrothion) on some aspects of carbohydrate metabolism in a freshwater fish, Sarotherodon (Tilapia) mossambicus (Peters)

A lethal (Lc50/48 h - 6 mg/l) concentration of the organophosphate (OP) pesticide Sumithion increased blood glucose levels and phosphorylase activity, but hepatic glycogen registered a fall which indicated that the observed hyperglycemia was due to breakdown of hepatic glycogen.     

Effect of organophosphate pesticide Sumithion (Fenitrothion) on some aspects of carbohydrate metabolism in a freshwater fish,Sarotherodon (Tilapia)mossambicus (Peters)

Summary A lethal (Lc₅₀/48 h — 6 mg/l) concentration of the organophosphate (OP) pesticide Sumithion increased blood glucose levels and phosphorylase activity, but hepatic glycogen registered a fall which indicated that the observed hyperglycemia was due to breakdown of hepatic glycogen.This work was supported by UGC grant to R.R.     

Purification and properties of a heat-resistant exotoxin produced byMacrophomina phaseolina (Tassi) Goid in culture

Summary A partially purified preparation of a water-soluble, heat-resistant, nonspecific exotoxin produced by a strain ofMacrophomina phaseolina, isolated fromPhaseolus mungo L. could reduce Cu⁺⁺ ions and produced a red colour with 2,4-dinitrophenyl hydrazine reagent. It caused inhibition of seed germination, wilting of cut seedlings, stunted growth of young seedlings and loss of permeability of the cell membrane. Seedlings ofP. mungo, grown in presence of the toxin showed a slight increase in the contents of protein and total RNA over control, but a significant increase in the specific activities of F-1, 6-BP aldolase and G-6-P isomerase.     

Chromosomes of some Asian and South American squirrels (rodentia: Sciuridae)

Zusammenfassung Chromosomensätze verschiedener Sciurinae sind:Sciurus anomalus (2n=40),S. granatensis (2n=42),Callosciurus flavimanus (2n=40),Dremomys rufigenis (2n=38) undMenetes berdmorei (2n= 62). Ein Vergleich mit früher beschriebenen Sciurinae-Karyotypen ergibt als ursprünglich diploide Sätze 38–40, Spaltung, sowie Fusion zu grösseren und kleineren2n's führend.     

Synthesis and taste properties of anN-(glucose)-saccharin compound

Résumé La saccharine a été fixée par son azote imidique sur le C₆ du glucose, par l'action de la saccharine sodée sur un dérivé protégé dup-toluénesulfonyl-6-O-d-glucose, et élimination des groupes protecteurs pour former un composé hydrosoluble. La saveur amère peut être attribuée à l'élimination du proton imidique de la saccharine.     

Purification and some properties of an amine oxidase from soybean seedlings

Summary An amine oxidase was purified 447-fold from soybean seedlings and some of its properties were investigated. The molecular weight of the enzyme was estimated to be 25,000. It was most active towards putrescine, followed by spermidine and spermine. K_m-values for these substrates were relatively close. The enzyme was strongly inhibited by carbonyl reagents, such as semicarbazide and aminoguanidine.