A method to collect cervical smears from small breeds of monkeys.

A simple method is described to collect cervical smears and to have a clear view of cervix for small breed of monkeys. This method was found useful to collect adequate cytology smears with good preservation of cellular morphology.     

A rather accurate method to determine Vitamine Deficiency from Urine

Zusammenfassung Die Untersuchung der Ausscheidung im Urin nach Vitaminbelastung ist ein zur Bestimmung der Hypovitaminose geeignetes Verfahren, wenn wir die Belastungsdose in Abständen von je 2 Stunden darreichen und die Einzelausscheidungswerte kurvenmäßig darstellen. Die Kurven ermöglichen eine Differenzierung zwischen Hypovitaminose und Ausscheidungsstörung und die Beurteilung des Grades der Hypovitaminose.     

A new method for obtaining blood from a small mammal without injuring the animal: use of Triatomid bugs

Summary By means of hungry Triatomid bugs, samples of blood (40–200l) can be obtained from small mammals, such as bats, without causing any harm to the animals. The blood can be extracted from the gaster of the bug immediately after the bug has finished sucking. The method was successfully used for measuring the energy budget by the doubly labeled water method, and for obtaining material for lymphocyte cultures, and may prove useful in other fields, too.M. Volleth is very much obliged to Prof. Dr R. A. Pfeiffer for giving her the opportunity to work at the Institute of Human Genetics.     

A simple technique for obtaining carbon replicas from small organisms

Résumé Exposé d'une méthode permettant de faire des répliques au carbone de petits organismes tel que les aphides. L'utilisation d'un substrat de technovit^® pour fixer les organismes est la partie importante de cette méthode. Le technovit a la propriété de coller la pellicule de carbonne; ainsi lorsque l'on détache celle-ci à l'aide de collodion, on obtient seulement la réplique se trouvant sur l'organisme.     

The transcendental method from Newton to Kant

Kant’s transcendental method, as applied to natural philosophy, considers the laws of physics as conditions of the possibility of experience. A more modest transcendental project is to show how the laws of motion explicate the concepts of motion, force, and causal interaction, as conditions of the possibility of an objective account of nature. This paper argues that such a project is central to the natural philosophy of Newton, and explains some central aspects of the development of his thinking as he wrote the Principia. One guiding scientific aim was the dynamical analysis of any system of interacting bodies, and in particular our solar system; the transcendental question was, what are the conceptual prerequisites for such an analysis? More specifically, what are the conditions for determining “true motions” within such a system—for posing the question of “the frame of the system of the world” as an empirical question? A study of the development of Newton’s approach to these questions reveals surprising connections with his developing conceptions of force, causality, and the relativity of motion. It also illuminates the comparison between his use of the transcendental method and that of Euler and Kant.     

A radioenzymatic method to measure picogram amounts of dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) in small samples of brain tissue

Summary A radioenzymatic method for simultaneous determination of dopamine and DOPAC in small brain areas is described. By using this assay, 250 pg of dopamine and 150 pg of DOPAC can be estimated. The present method has been applied to compare the effect of different psychotropic drugs on the dopamine and DOPAC levels in the caudate nucleus, substantia nigra and medial basal hypothalamus.     

A simple method enabling standardized handling of small biological objects for light and electron microscopic preparation

Summary A glass filtration-apparatus provided with an exchangeable nylon-tissue filter permits collection, fixation, dehydration, staining and preembedding of small biological objects in a simple and rapid way.This work was supported by grant No 3.218.-077 of the Swiss National Science Foundation.     

A method for the isolation of ommochrome-containing granules from insect eyes

Summary A routine method for the preparation of pigment granules from insect eyes is reported. When viewed using an electron microscope, the isolated granules appear to be free from different structural elements, and there is very little contamination by membranous material. Ultrastructure and extinction spectra correspond to those found in situ.Acknowledgments. We thank Prof. Dr A. Fischer and Dr J. Rosenberg for their help in electron microscopic techniques. This work was supported by the Deutsche Forschungsgemeinschaft.     

A rapid electrophoretic method for the separation of hydroxyproline from proline

Summary A rapid, sensitive procedure is described which uses custom made apparatus to separate radioactive imino acids. This technique provides a simple measurement of the distribution of newly synthesized collagen in different fractions.Acknowledgment. This work was supported by grants from the International Wool Secretariat.     

A simple method for the collection of leucocytes from rat blood

Zusammenfassung Blutleukozyten von Ratten wurden mit menschlichem (normalem) Serum behandelt. Die Viabilität dieser Präparationen konnte dadurch demonstriert werden, dass die Zellen durch Phytohemagglutinin in «blast» Formen umgewandelt wurden.     

Primary tissue cultures from organ fragments: A simplified method

Summary Organ fragments washed in Ca⁺⁺ and Mg⁺⁺-free saline, treated with trypsin and placed directly into culture flasks adhered within seconds to the vessel surface. If the fragments were suspended in culture medium before they were added to the flasks, they did not adhere. This technique permits the rapid attachment and subsequent growth of the primary tissue cultures.Adapted from a thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at Florida Atlantic University.Acknowledgment. This work was carried out in the laboratory of J.X. Hartmann, whom I thank for his generosity and helpful discussion.     

混凝土坝裂缝转异诊断的顺序典型小概率法

裂缝是混凝土坝不可避免的病害,其稳定与否是关系到混凝土坝结构安全的关键因素.基于裂缝开度与裂缝尖端张开位移之间的函数关系,提出了混凝土坝裂缝转异诊断的临界裂缝开度准则,并探讨了该准则所需的裂缝尖端张开位移和裂缝亚临界扩展量的确定方法.借助裂缝开度监控模型将临界裂缝开度准则进行转化,并与大坝安全监控中裂缝的原位监测资料-裂缝开度联系起来,建立了混凝土坝裂缝转异诊断的顺序典型小概率法.实例分析表明,顺序典型小概率法实现了临界裂缝开度准则在大坝安全监控中的具体应用,是合理可行的.     

Preparation of serum for paper electrophoresis from small animals

Zusammenfassung 1 bis 2 Tropfen Blut werden in einer Kapillare 10 min bei 10 000 U/min zentrifugiert. Die Kapillare wird an der Schichtgrenze zerschnitten. Das Serum kann so leicht auf die Papierstreifen zur Elektrophorese aufgetragen werden.     

Ascorbic acid content of neotropical plant parts available to wild monkeys and bats

The ascorbic acid content of foliage available to wild primates and bats in Panama (in transition between wet and dry seasons) was lower than that of temperate zone foliage but higher than that of most fruits and vegetables. Intakes of ascorbic acid (mg/kg b.wt/day) by wild primates and frugivorous bats in Panama are much greater than that of most human populations.