The biliary excretion of [3H] lysergic acid diethylamide in Wistar and Gunn rats.

The biliary excretion of [3H] LSD was studied in Wistar and homozygous Gunn rats. In Wistar rats approximately 46% of the given dose was recovered from bile in 2.5 h whilst in the homozygous Gunn rat 26% was recovered in the same time period. In both strains the main metabolites were glucuronides.     

The subcellular distribution of lysergic acid diethylamide in the rat brain

Résumé Quinze min après une injection i.p. de LSD, près de 48% de la drogue présente dans le cerveau fut trouvée dans le surnageant, 20% dans la fraction nucléaire, 15% dans la fraction synaptosomale, 6% dans la fraction microsomale, 5% dans la fraction myélinique et près de 1,7% dans la fraction mitochondriale. Les fractions synaptosomale et mitochondriale sont presque saturées en LSD lors d'une injection de 10 mg/kg de LSD.

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Acknowledgment. I thank Dr.J. Knopp for his help with the spectrophotoflourometric analysis.     

Formation of omega-muricholic acid and fecal excretion of biliary acids in rats]

Two groups of Rats which were fed the same diet, excreted fecal bile acids of different patterns: in one group, the feces contained a large amount of omega-muricholic acid and very little hydrodeoxycholic acid; in the other group the reversed composition was observed. The first group excreted more total bile acids than the second. This observation suggests that the formation of a large amount of omega-muricholic acid results in an increase in total bile acid fecal excretion.     

Interaction of serotonin and lysergic acid diethylamide (LSD) in the central nervous system

Zusammenfassung Serotonin (5-Oxytryptamin) wirkt bei der Maus als mildes Sedativum und steigert den hypnotischen Effekt von Evipan deutlich. Diese Wirkungssteigerung kann durch Lysergsäure-diäthylamid (LSD) antagonisiert werden. Dieser Befund stimmt mit der Ansicht überein, dem Serotonin komme eine physiologische Rolle im Gehirn zu und dass LSD möglicherweise durch antagonistische Wirkung gegenüber Serotonin Psychose verursachen kann.     

Changes in the liver protein pattern due to lysergic acid diethylamide (LSD)

Riassunto Mediante elettroforesi in fase libera è stato studiato il protiogramma epatico durante l'intossicazione con dietilamide dell'acido lisergico (LSD) nel ratto albino.Si sono osservate profonde modificazioni del quadro le quali sonto completamente reversibili.     

Distribution and fate of14C-labeled lysergic acid diethylamide (LSD 25) in the animal body

Zusammenfassung Es wird die Verteilung, Umwandlung und Ausscheidung von intravenös verabreichtem¹⁴C-markiertem Lysergsäurediäthylamid (LSD) an der weissen Maus untersucht.Praktisch die ganze, in Form von¹⁴C-markiertem LSD applizierte Radioaktivität verschwindet innerhalb weniger Minuten aus dem Blut, um nach 10–15 min in den meisten Organen ein Maximum zu erreichen, aus denen sich die Radioaktivität in den nächsten Stunden langsam wieder verliert. Im Dünndarm steigt die Aktivität in den ersten 2 h zu einem Maximum an, das ungefähr 50% der totalen Dosis entspricht. Die radioaktive Substanz bewegt sich mit dem Darminhalt, und 2–3 h später wird der grösste Teil der Aktivität im Colon gefunden.Durch Extraktionsversuche unter Zusatz von inaktivem LSD wird gezeigt, dass der grösste Teil des LSD im Organismus chemisch verändert wird. Drei verschiedene, wasserlösliche radioaktive Abbauprodukte werden durch ihreR _f-Werte charakterisiert.     

Human platelet 5-hydroxytryptamine receptors: binding of [3H]-lysergic acid diethylamide (LSD). Effects of chronic neuroleptic and antidepressant drug administration

Chronic treatment with phenothiazines and thioxanthenes has been found to enhance 5-HT-induced aggregation of human platelets. A method has been developed to study 5-HT2 receptor binding sites on platelets utilising [3H]-LSD and more recently 125I/LSD. Results are presented which suggest that the LSD binding site is indeed the 5-HT2 binding site and that the LSD binding characterises the specific receptor responsible for 5-HT-induced shape change and aggregation. In a group of patients receiving phenothiazines or thioxanthenes, the Bmax of LSD binding was increased. The mean binding affinity was decreased possibly due to a persistence of neuroleptic in the platelet membrane preparation. Analysis showed that this was not the reason why the mean binding capacity was increased. The results show that chronic phenothiazine and thioxanthene delta treatment 'up-regulates' platelet 5-HT2 binding sites and that this may be accompanied by increased sensitivity to platelet aggregation by 5-HT. In normal subjects desipramine treatment increased the Bmax of platelet LSD binding and this was accompanied by an increased prolactin response to tryptophan which is thought to be mediated by central 5-HT function.     

Vitamin D and reproduction in Wistar rats]

Females have received from weaning a semi-synthetic diet (0,35% Ca; 0,32% P; 0,03% Mg), with or without vitamin D (+/- D). Fertility of these females was not changed. At weaning, the young from mothers--D had lower weight, calcemia and phosphatemia, but the ash content of the femur was the same as for young from mothers + D. Young rats from mothers +/- D were given the diet with or without vitamin D. 19 days after weaning, the diet of the mother (+/- D) appears to be the principal factor for growth, calcemia and the ash content of the femur; the diet of the young (+/- D) produced only slight differences between ash contents.     

Acid excretion in young and adult Wistar Kyoto and spontaneously hypertensive rats

Summary Wistar Kyoto rats (WKy), the most widely accepted control for SH rats, show an inability to excrete acid appropriately when compared to another normotensive strain, SD. Coupled with the fact that KWy also develops sodium-sensitive hypertension, this makes them a more complex control than realized. At very young ages (<10-week-old), neither SH nor WKy show any deficiency in acid excretion.Acknowledgments. Supported by AHA grant 76 1019 and NIH grant AM 15458. The excellent secretarial help of Susan Dreux and Betty Mendelson is acknowledged.     

Decrease in [3H]ouabain binding sites in heart and brain from spontaneously hypertensive rats

Summary A decrease in the number of binding sites (B_max) for [³H]ouabain was observed in the heart (37%) and the brain (22%) of spontaneously hypertensive rats (SHR) when compared with age-matched control Wistar Kyoto rats. No variation was detected in the affinity constant (K_D).     

Acid excretion in young and adult Wistar Kyoto and spontaneously hypertensive rats.

Wistar Kyoto rats (WKy), the most widely accepted control for SH rats, show an inability to excrete acid appropriately when compared to another normotensive strain, SD. Coupled with the fact than KWy also develops 'sodium-sensitive' hypertension, this makes them a more complex control than realized. At very young ages (less than 10-week-old), neither SH nor WKy show any deficiency in acid excretion.     

Evidence of catecholamine mediation in the ‘Aberrant’ behaviour induced by lysergic acid diethylamide (LSD) in the rat

Zusammenfassung Der Einfluss verschiedener Pharmaka auf das durch LSD hervorgerufene bizarre Verhalten der Ratte wurde untersucht. Die Resultate zeigen, dass - und -Blocker sowie -Methyltyrosine eine starke Hemmung dieses Verhaltens erzeugen, im Gegensatz zu den Serotonin-Antagonisten Deseril, BOL-148 und Parachlorphenylalaninen, die nur schwache oder keine Hemmung hervorrufen. Es wird gefolgert, dass das bizarre Verhalten durch Wirkung von LSD an Katecholaminstrukturen hervorgerufen wird. Die Wirkung von 4 psychotropen Pharmaka am bizarren Verhalten wird im Rahmen dieser Hypothese diskutiert.