毛细管区带电泳法分离检测尿液中的盐酸克伦特罗

建立了一种测定人体尿液中盐酸克伦特罗的毛细管区带电泳一紫外检测方法,考察了实验参数对分离和检测结果的影响．在10mmol／L的硼酸盐缓冲溶液（pH=10．0）,在进样电压～2kV×2s,分离电压～18．5kV,检测波长210nm,分离温度32％的最佳条件下,盐酸克伦特罗与人体尿液中干扰物质能够在2rain内完全分离．其最低检测限（以信噪比为3计）为0．5mg／L,线性相关系数（R。）为0．9986,加标回收率为86．0％～94．0％,相对标准偏差（RSD）为1．7％～2．9％．所建立的方法直接用于尿液中盐酸克伦特罗的测定,结果令人满意．     

盐酸克伦特罗的电化学发光检测器的研究

基于2 [(叔丁氨基)甲基] 4 氨基 3,5 二氯苯甲醇酸盐(盐酸克伦特罗)对联吡啶钌(Ru(bpy)2 3)电化学发光信号有较强的增敏作用,建立了一种检测盐酸克伦特罗的电化学发光新方法.结果表明,在0.1mol/L的Na2HPO4介质中,以循环伏安为电解方式,联吡啶钌的浓度为1 0×10-6g/mL时,盐酸克伦特罗对联吡啶钌电化学发光信号的增敏效果最好,在此条件下,该方法对盐酸克伦特罗的线性范围为6.0×10-7～9.0×10-6g/mL,检出限为7.3×10-8g/mL,相对标准偏差RSD为3 9%(n=7,c=1 0×10-6g/mL).     

肉制品中β-兴奋剂的毛细管电泳-电化学检测方法

首次采用毛细管电泳-电化学检测法（CE-ED）同时测定了肉制品中β-兴奋剂盐酸克伦特罗与沙丁胺醇的含量,考察了缓冲液酸度和浓度、分离电压、氧化电位和进样时间等实验参数对分离检测的影响.在最佳实验条件下,工作电极为直径300 μm的碳圆盘电极,检测电位为+950 mV（vs. SCE）,缓冲液为40 mmol/L硼砂溶液（pH =7.4）,分离电压 18 kV,上述组分在8 min中内即可实现分离.盐酸克伦特罗和沙丁胺醇在两个数量级的范围内呈良好线性关系,检测下限（S/N=3）分别为1.11×10-7和9.80×10-8 mol/L,且抗坏血酸对其检测不产生干扰.该方法已成功地应用于实际样品中β-兴奋剂残留状况的分析,结果令人满意.     

固相萃取—HPLC-PDA 法测定猪肉中的盐酸克伦特罗的研究

对固相萃取——HPLC-PDA法测定猪肉中的盐酸克伦特罗的前处理方法和色谱条件进行了优化.超声提取时间30min,固相萃取柱净化过柱速度为2d/s.色谱条件为V甲醇∶V0.01 mol/L磷酸二氢钠溶液=35∶65,固定相ODS-C18,高效液相色谱柱4.6μm×150 mm,流速1 mL/min,检测波长243 nm.优化试验条件后盐酸克伦特罗残留量含量回收率得到较大改善,该方法检测线性良好,操作简单、快速,色谱峰形理想,结果准确可靠,重现性佳     

离子色谱法同时测定饮用水中的氯化物、硝酸盐氮和硫酸盐

采用离子色谱法 ,Shim -PackIC—A1阴离子柱 ,以 2 .5mmol/L邻苯二甲酸 + 2 .4mmol/L三 (羟甲基 )氨基甲烷为流动相 ,同时测定饮用水中氯化物、硝酸盐氮和硫酸盐的含量 ,回收率为 99%～ 10 2 % ,相对标准偏差均 <3 % ,最低检测限分别为 0 .0 10mg/L、0 .0 0 5mg/L、0 .0 2 0mg/L。实验证明 ,此方法快速、简便、准确、灵敏度高     

流动注射化学发光法测定盐酸米托蒽醌

在酸性条件下,甲醛与高锰酸钾能够产生较弱的化学发光,而盐酸米托蒽醌能够大大地增强该化学发光强度.在一定浓度范围内,增加的发光强度与盐酸米托蒽醌的浓度呈良好的线性关系.由此建立了一种测定盐酸米托蒽醌的流动注射化学发光新方法,方法的检出限为6·4μg/L(IUPAC),线性范围为0·01~1·0mg/L,对0·5mg/L盐酸米托蒽醌平行测定11次,其相对标准偏差为2·8%.     

毛细管电泳-电化学发光法测定盐酸西替利嗪的含量

采用毛细管电泳-联吡啶钌电化学发光法测定了盐酸两替利嗪的含量.讨论了联吡啶钌浓度、检测电位、磷酸盐缓冲液浓度和pH值、进样时间和进样电压等实验条件对盐酸西替利嗪分离检测的影响.在优化实验条件下,盐酸西替利嗪在10～140 mg/L范围内呈良好线性(相关系数0.997 6);检出限为2.0 mg/L(S/N=3).本法可直接用于尿液中盐酸西替利嗪含量的测定,回收率为96.9%～98.7%.     

离子色谱在水质分析中的应用

文章采用离子色谱法, Shim - PackICAl 阴离子柱,以2. 5mmol/L 邻苯二甲酸 2 4mmol/L三(羟甲基)氨基甲烷为流动相,同时测定饮用水中氯化物、硝酸盐氮和硫酸盐的含量。该法回收率为99%~102%,相对标准偏差均<3%,最低检测限分别为0 010mg/L、0 005mg/L、0.020mg/L。实验证明该方法快速、简便、准确、灵敏度高。     

醋酸尼泼松对皮状丝孢酵母产微生物油脂的影响

研究了醋酸泼尼松对皮状丝孢酵母生长和油脂积累的影响.结果表明:添加醋酸泼尼松100mg/L,可以有效促进皮状丝孢酵母的生长,使皮状丝孢酵母生物量达到最大,比未添加醋酸泼尼松提高68%;一次添加醋酸泼尼松200mg/L,对皮状丝孢酵母油脂积累油脂最为明显,可以提高98%的产量;如果采用分批添加醋酸泼尼松200mg/L,即30h前使添加醋酸泼尼松维持100mg/L,30h后补加醋酸泼尼松,维持在200mg/L,微生物油脂产量可以从最初2.5g/L提高到5.7g/L,产量提高128%;通过气相检测发现醋酸泼尼松的添加对酵母油脂脂肪酸的组成含量也有影响,油酸、亚油酸含量分别增加21.6%、32.3%.本文为提高皮状丝孢酵母生物量和油脂积累提供了一种新的方法.     

顺序注射分析法测定自来水中硫酸盐

建立了在线沉淀顺序注射分析法测量了自来水中硫酸盐的方法.沉淀稳定剂:氯化钠-盐酸-甘油-乙醇和氯化钡;载液:蒸馏水.对沉淀体系中影响显色的诸因素进行了实验研究.方法检测限为10mg/L,线性范围10～1000mg/L.r=0.9993.对65.6mg/L自来水样品进行11次平行测量,测得相对标准偏差为3.3%.回收率97.2%.检测速度70次/h.结果:方法准确、可靠,具有实用价值.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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