| 基于金钗石斛EST的短串联重复序列的挖掘 |
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| 引用本文： | 梁山, 陈莉莉, 李洪清. 基于金钗石斛EST的短串联重复序列的挖掘[J]. 华南师范大学学报（自然科学版）, 2011, (2). |
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| 作者姓名： | 梁山 陈莉莉 李洪清 |
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| 作者单位： | 1.1.华南师范大学;2.2. |
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| 基金项目： | 国家自然科学基金-广东省联合基金项目，广东省博士启动基金项目，广东省植物发育重点实验室开放基金 |
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| 摘 要： | 金钗石斛是常见的药用和花卉植物，但是缺乏在基因组尺度上对该物种的研究.本研究利用计算机程序搜索了金钗石斛EST上的短串联重复位点，共获得2 122个基序长度2～7bp,STR长度不小于12bp且重复次数不小于3的STR位点. 其中，3bp-STR最为丰富，而 2bp和6bp基序的STR位点也在可表达基因中富集.一般而言，随着基序长度的增加，STR的重复次数和分布范围减少/缩窄. 金钗石斛基因组上存在439个基因特异分布的STR位点，暗示这些STR位点可能与特定的功能基因共进化.
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| 关 键 词： | 金钗石斛，短串联重复，表达序列标签 |
| 收稿时间： | 2010-11-05 |
| 修稿时间： | 2010-12-02 |
EST-STR MINING FROM DENDROBIUM NOBILE |
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| EST-STR MINING FROM DENDROBIUM NOBILE[J]. Journal of South China Normal University (Natural Science Edition), 2011, (2). |
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| Authors: | LIANG Shan CHEN Lili LI Hongqing |
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| Abstract: | Dendrobium nobile is a herb and floral plant, but the studies at the genomic scale on this species are rare. Through the computer-facilitated data mining, a total of 2122 short tandem repeat (STR) loci that contain motifs in length ranged from 2bp to 7bp and with 3 or more repeats were identified from an EST collection of Dendrobium nobile . Analysis on the STR frequency indicates that group of 3bp-STR is most abundant, while those with 2bp- and 6bp-motif also enriched in expressed genes. Generally, the number of repeats and the distribution range of STRs decreases with the length of motif. A total of 439 STRs are gene-specific hosted, suggesting a co-evolution between these STR loci and the specific functional genes. These results will facilitate the comparative studies between Dendrobium nobile and other plants at the genomic scale. |
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| Keywords: | Dendrobium nobile，short tandem repeat (STR)，EST |
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