| 流动注射化学发光法测定阿卡波糖 |
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| 引用本文： | 赵冕,王贝贝,王冰,李献锐. 流动注射化学发光法测定阿卡波糖[J]. 河北大学学报(自然科学版), 2022, 42(3): 251. DOI: 10.3969/j.issn.1000-1565.2022.03.005 |
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| 作者姓名： | 赵冕 王贝贝 王冰 李献锐 |
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| 作者单位： | 河北医科大学教学实验中心,河北石家庄 050017;河北医科大学药学院,河北石家庄 050017 |
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| 基金项目： | 河北省自然科学基金资助项目(B2020206009);河北省高等学校科学技术研究项目(QN2021101) |
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| 摘 要： | 建立一种流动注射化学发光法测定阿卡波糖的新方法.在碱性条件下,阿卡波糖对H2O2-鲁米诺发光体系的发光强度具有显著的增敏作用,在最佳实验条件下,发光强度的增加值(ΔI)与阿卡波糖的质量浓度在3.0×10-4~2.0×10-2 mg/mL内成线性关系,相关系数为0.998 7.方法的检出限为2.3×10-4 mg/mL,对0.01 mg/mL的阿卡波糖连续测定11次,其相对标准偏差为2.7%,并应用于阿卡波糖片剂含量的测定,平均回收率为101.6%.本方法操作简单快速,重现性好,可用于阿卡波糖的含量测定.
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| 关 键 词： | 阿卡波糖 流动注射 化学发光 增敏 |
| 收稿时间： | 2021-10-20 |
Flow injection chemiluminescence method for determination of acarbose |
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| ZHAO Mian,WANG Beibei,WANG Bing,LI Xianrui. Flow injection chemiluminescence method for determination of acarbose[J]. Journal of Hebei University (Natural Science Edition), 2022, 42(3): 251. DOI: 10.3969/j.issn.1000-1565.2022.03.005 |
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| Authors: | ZHAO Mian WANG Beibei WANG Bing LI Xianrui |
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| Affiliation: | 1.Experimental Center for Teaching, Hebei Medical University, Shijiazhuang 050017, China; 2.College of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China |
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| Abstract: | A simple and rapid flow-injection chemiluminescence(FI-CL)method was developed for the determination of acarbose in tablets based on the fact that acarbose can remarkably enhance the chemiluminescence of Luminol-H2O2 in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence relative intensity had a good linear relationship with acarbose concentration in the range of 3×10-4~2×10-2 mg/mL with a correlation coefficient of 0.998 7. The detection limit was 2.3×10-4 mg/mL and RSD(n=11)is 2.7% for 0.01 mg/mL acarbose. The proposed method has been successfully applied for the determination of acarbose in tablets sample and the average recovery was 101.6%. |
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| Keywords: | acarbose flow injection chemiluminescence sensitization |
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