| 生物酶对五氯联苯降解的初步研究 |
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| 引用本文： | 生物酶对五氯联苯降解的初步研究. 生物酶对五氯联苯降解的初步研究[J]. 山东科学, 2016, 29(6): 94-97. DOI: 10.3976/j.issn.1002-4026.2016.06.015 |
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| 作者姓名： | 生物酶对五氯联苯降解的初步研究 |
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| 作者单位： | 山东省科学院生态研究所, 山东省应用微生物重点实验室, 山东 济南 250014 |
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| 基金项目： | 国家国际科技合作专项（2014DFE90100）；山东省自然科学基金（ZR2015YL008, BS2015HZ011） |
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| 摘 要： | 多氯联苯（PCBs）是具有代表性的持久性有机污染物（POPs），其氯代数目越多，降解越难。本文以分离筛选的6株PCBs降解细菌为材料，制备了含有NADH辅酶再生系统关键酶甲酸脱氢酶（FDH）的PCBs降解复合酶，研究了其对PCBs的降解效果。结果表明，Pseudomonas sp. MGB11胞内酶/FDH复合酶对五氯联苯PCB114的降解率在10 h内可达69.4%，对于高毒性、难降解的高氯PCBs的污染修复具有应用价值。
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| 关 键 词： | 降解复合酶 五氯联苯 甲酸脱氢酶 |
| 收稿时间： | 2016-08-08 |
Pilot study on bioenzyme degradation to pentachlorodiphenyl |
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| JI Lei,CHEN Guan-hong,FU Xiao-wen,HUAGN Yu-jie,WANG Jia-ning,ZHANG Qiang. Pilot study on bioenzyme degradation to pentachlorodiphenyl[J]. Shandong Science, 2016, 29(6): 94-97. DOI: 10.3976/j.issn.1002-4026.2016.06.015 |
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| Authors: | JI Lei CHEN Guan-hong FU Xiao-wen HUAGN Yu-jie WANG Jia-ning ZHANG Qiang |
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| Affiliation: | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 250014, China |
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| Abstract: | Polychlorinated biphenyls (PCBs) is a representative of persistent organic pollutants (POPs). More its chlorinated number leads to more difficult its degradation . We prepared PCBs compound degrading enzyme of key enzyme formate dehydrogenase (FDH) of NADH coenzyme regeneration system with isolated 6 PCBs degradation bacteria strains as materials. We address its degrading effect to PCBs. Results indicate that degradation rate of Pseudomonas sp. MGB11/FDH compound enzyme to pentachlorodiphenyl PCB114 is up to 69.4% within 10 h. It therefore has application value in the repair for high chlorine PCBs pollutants with high toxicity and low degradability. |
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| Keywords: | pentachlorodiphenyl formate dehydrogenase compound degrading enzyme |
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