| 霜霉病高效生防菌株BCJB01发酵培养基的优化 |
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| 引用本文： | 霜霉病高效生防菌株BCJB发酵培养基的优化. 霜霉病高效生防菌株BCJB01发酵培养基的优化[J]. 山东科学, 2016, 29(4): 30-34. DOI: 10.3976/j.issn.1002-4026.2016.04.007 |
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| 作者姓名： | 霜霉病高效生防菌株BCJB发酵培养基的优化 |
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| 作者单位： | 山东省科学院生物研究所，山东省应用微生物重点实验室，山东 济南 250014 |
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| 基金项目： | 山东省重点研发计划（2015GNC113002）；山东省科学院-枣庄市产业技术研发联合基金（201503）；公益性行业( 农业) 科研专项(201203035) |
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| 摘 要： | 为提高霜霉病高效生防菌株Bacillus cereus BCJB01的发酵水平，通过正交试验、综合优选及糖分流加试验，优化BCJB01的发酵培养基。优化出的培养基配方的质量分数为：玉米粉2.00%、豆粕粉1.00%、四水氯化锰0.003 0%、十二水磷酸氢二钠0.30%、二水磷酸二氢钠0.15%、七水硫酸镁0.035%、氯化钙0.10%和酵母提取物0.30%，接菌后6～12 h流加质量分数0.2%糖。本研究优化的培养基配方在接种量为3%（V/V），转速为180 r /min，温度为 30 ℃的摇床振荡培养至66 h时，芽孢率近90%，菌体数量达100×108 cfu/mL以上。
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| 关 键 词： | 蜡样芽孢杆菌 正交设计 综合优选 霜霉病 发酵培养基 |
| 收稿时间： | 2016-04-27 |
Optimization of fermentative medium for highly effective biocontrol strainBacillus cereus BCJB01 against downy mildew |
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| WANG Yi-lian,XIE Xue-ying,HU Jin-dong,CHEN Kai,YANG He-Tong,LI Ji-shun. Optimization of fermentative medium for highly effective biocontrol strainBacillus cereus BCJB01 against downy mildew[J]. Shandong Science, 2016, 29(4): 30-34. DOI: 10.3976/j.issn.1002-4026.2016.04.007 |
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| Authors: | WANG Yi-lian XIE Xue-ying HU Jin-dong CHEN Kai YANG He-Tong LI Ji-shun |
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| Affiliation: | Shandong Provincial Key Laboratory of Applied Microbiology, Biology Institute, Shandong Academy of Sciences, Jinan 250014, China |
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| Abstract: | To improve fermentation level of Bacillus cereus BCJB01, a highly efficient biocontrol strain against downy mildew, we optimized its fermentative medium through orthogonal test, comprehensive optimization and sugar feeding tests. Mass fraction of the optimized medium formula are 2.00% corn meal,1.00% soybean powder, 0.0030% manganese chloride tetrahydrate, 0.30% twelve aqueous disodium hydrogen phosphate, 0.15% sodium dihydrogen phosphate dihydrate, 0.035% magnesium sulfate heptahydrate, 0.10% calcium chloride, 0.30% yeast extract, 0.2%sugar added at 6~12 h after inoculation. It can produce more than 100×108 cfu/mL cells and its spore rate is 90%, when the cultivation conditions are 3%(V/V) inoculum size, 180 r / min rotation velocity, 30 ℃ shaky cultivation till 66 hours. |
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| Keywords: | orthogonal design downy mildew Bacillus cereus fermentative medium comprehensive optimization |
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