| 蛇足石杉的组织培养初探 |
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| 引用本文： | 周颖,刘星,李克刚,王正功,耿欣,胡颂平. 蛇足石杉的组织培养初探[J]. 吉首大学学报(自然科学版), 2009, 30(2): 90-93 |
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| 作者姓名： | 周颖 刘星 李克刚 王正功 耿欣 胡颂平 |
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| 作者单位： | 湖南省吉首大学省部共建生物工程实验室,湖南,吉首,416000;岳阳职业技术学院生物工程系,湖南,岳阳,414000 |
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| 基金项目： | 湖南省科技计划项目,上海市农业生物基因中心资助项目 |
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| 摘 要： | 针对蛇足石杉资源分散，资源更新周期长，难以大规模开采的情况，初步探讨了蛇足石杉的组织培养技术.以蛇足石杉茎尖为外植体，分别在1/2MS+0.05 μmol/L IBA+1.4 μmol/L KT+2 mg/L谷氨酰胺、MS+0.05 μmol/L IBA+1.4 μmol/L KT+2 mg/L谷氨酰胺、1/2MS+2 mg/L谷氨酰胺/无激素、MS+2 mg/L谷氨酰胺/无激素等4种培养基中培养.结果显示:较为适宜的初代培养基为2号培养基,即MS+0.05 μmol/L IBA+1.4μ mol/L KT+2 mg/L谷氨酰胺.在组织培养工作中，污染问题比较严重，本实验中，采用了以下2种方法对外植体进行消毒：（1）70%酒精浸泡1 min→5% NaOCl浸泡1 min→7%H2O2浸泡10 min;（2）0.1%升汞液灭菌3’ →无菌水冲洗5次.结果显示第2种方法比较理想.
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| 关 键 词： | 蛇足石杉 组织培养 愈伤组织 |
Tissue Culture of Huperzia Serrata |
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| ZHOU Ying,LIU Xing,LI Ke-gang,WANG Zheng-gong,GENG Xin,HU Song-ping. Tissue Culture of Huperzia Serrata[J]. Journal of Jishou University(Natural Science Edition), 2009, 30(2): 90-93 |
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| Authors: | ZHOU Ying LIU Xing LI Ke-gang WANG Zheng-gong GENG Xin HU Song-ping |
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| Affiliation: | (1.College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China;2.Biological Engineering Department,Professional Technological College of Yueyang,Yueyang 414000,Hunan China) |
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| Abstract: | In this study,tissue culture was explored to solve the propagate problem in the production with Huperzia serrata(Thunb)Trev.The stem and shoot-tips were used as explants to induce callus.These explants are cultured in four culture mediums:(1) 1/2MS+0.05 μmol/L IBA+1.4μmol/L KT+2 mg/L pirydoxine;(2) MS+0.05 μmol/L IBA+1.4 μmol/L KT+2 mg/L pirydoxine;(3) 1/2MS+2 mg/L pirydoxine;(4) MS+2 mg/L pirydoxine.The results show that the suitable element compositions to induce callus is:MS+0.05 μmol/L IBA+1.4μmol/L KT+2 mg/L pirydoxine.During tissue culture,fungal pollution is very serious,so the surface disinfection is important.There are two variants of surface sterilization.In the first variant shoot-tips were soaked in 70% ethyl alcohol (C2H5OH) for 1 min,5% sodium hypochlorite (NaOCl) for 1 min and then in 7% hydrogen peroxide (H2O2) for 10 min;the second variant were soaked in HgCl for 3 min.The result shows that the most suitable procedure for surface sterilization of explants is the second one. |
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| Keywords: | Huperzia serrata(Thun)Trev')" href=" #" >Huperzia serrata(Thun)Trev tissue culture callus |
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