| 人组氨酰-tRNA合成酶基因的克隆与表达 |
| |
| 引用本文： | 毕智丽,吴亦红,赵晓瑜,倪志华,王三元. 人组氨酰-tRNA合成酶基因的克隆与表达[J]. 河北大学学报(自然科学版), 2005, 25(3): 318-322. DOI: 10.3969/j.issn.1000-1565.2005.03.019 |
| |
| 作者姓名： | 毕智丽 吴亦红 赵晓瑜 倪志华 王三元 |
| |
| 作者单位： | 1.河北大学,生命科学学院,河北,保定,071002； 2.河北省环境科学研究院,河北,石家庄,050051 |
| |
| 摘 要： | 用RT-PCR方法从人胎盘总RNA中获得编码人组氨酰-tRNA合成酶(Jo-1)基因的全序列,选用IMPACT-CN系统中的pTYB11,构建克隆与表达载体,转化大肠杆菌ER2566.经筛选与鉴定,得到3个阳性重组子,对其进行诱导表达获得Jo-1融合蛋白,western-blotting检验结果显示,该融合蛋白具有Jo-1免疫原性和免疫特异性.
|
| 关 键 词： | 人组氨酰-tRNA合成酶 基因克隆 表达 自身抗原 |
| 文章编号： | 1000-1565(2005)03-0318-05 |
| 修稿时间： | 2004-10-20 |
Cloning and Expression of Human Histidyl-tRNA Synthetase |
| |
| BI Zhi-li,WU Yi-hong,ZHAO Xiao-Yu,NI Zhi-hua,WANG San-yuan. Cloning and Expression of Human Histidyl-tRNA Synthetase[J]. Journal of Hebei University (Natural Science Edition), 2005, 25(3): 318-322. DOI: 10.3969/j.issn.1000-1565.2005.03.019 |
| |
| Authors: | BI Zhi-li WU Yi-hong ZHAO Xiao-Yu NI Zhi-hua WANG San-yuan |
| |
| Affiliation: | BI Zhi-li1,WU Yi-hong2,ZHAO Xiao-yu1,NI Zhi-hua1,WANG San-yuan1 |
| |
| Abstract: | Using RT-PCR technology the full length DNA sequence coding for human histidyl-tRNA synthetase was obtained. The pTYB11 was used in construction of expressing vector. The recombinant plasmid constructed was transformed into ER2566 and the system was used to express fusion Jo-1 antigen. At last, the author got 3 recombinants that could successfully express the fusion protein. Meanwhile, the expressed fusion protein could be identified by western-blotting using anti-Jo-1 antibody. |
| |
| Keywords: | human histidyl-tRNA synthetase gene cloning expression autoantigen |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《河北大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《河北大学学报(自然科学版)》下载全文 |
|
