|
|||||
|
|
| 运用MuPS标记识别马尾松抗松材线虫病个体 | |
| 引用本文： | 高景斌,徐六一,叶建仁,矶田圭哉,生方正俊. 运用MuPS标记识别马尾松抗松材线虫病个体[J]. 南京林业大学学报(自然科学版), 2009, 0(3): 1-4 |
| 作者姓名： | 高景斌 徐六一 叶建仁 矶田圭哉 生方正俊 |
| 作者单位： | 1. 南京林业大学森林资源与环境学院,江苏,南京,210037;安徽省松材线虫抗性育种中心,安徽,合肥,230031 2. 安徽省松材线虫抗性育种中心,安徽,合肥,230031 3. 南京林业大学森林资源与环境学院,江苏,南京,210037 4. 日本森林综合研究所林木育种中心,日本,茨城县,3191301 |
| 基金项目： | 日本国际协力机构(JICA)援助项目 |
| 摘 要： | 运用14个SCAR标记引物对松材线虫病马尾松(Pinus massoniana)抗性候选个体开展MuPS(Multiplex PCR of SCAR markers)分析。结果表明，120个样品中出现104个不同的MuPS型，区分率86.7%、74.2%的个体完全能够被识别。引物Pmscar157、Pmscar007、Pmscar053的多态位点数出现率非常高，分别占总位点数的131%、13.3%、18.4%，而引物Pmscar039、Pmscar499的位点数出现率非常低，分别占总位点数的0.16%、048%。因引物重复性高、稳定可靠，可通过获得的MuPS型识别抗性候选个体、管理抗性候选家系。 |
| 关 键 词： | 松材线虫 马尾松 抗性选育 MuPS |
Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease |
|
| GAO Jing-bin,XU Liu-yi,YE Jian-ren,ISODA Keiya,UBUKATA Masatoshi. Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease[J]. Journal of Nanjing Forestry University(Natural Sciences ), 2009, 0(3): 1-4 | |
| Authors: | GAO Jing-bin XU Liu-yi YE Jian-ren ISODA Keiya UBUKATA Masatoshi |
| Affiliation: | 1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Anhui Province ForestryBureau Resistance Breeding for Pine nematode Centre, Hefei 230031, China; 3.Forest Tree Breeding CenterForestry Research Products Research Institute, Ibaraki 3191301, Japan |
| Abstract: | Using 14 SCAR marker primers to analyze the resistance seedlings of Pinus massoniana which resist Bursaphelenchus xylophilus.The results indicated that there were 104 different types of MuPS among those 120 samples,which could distinguish 86.6% samples,and 74.2% samples identified completely.Some primers such as Pmscar157,Pmscar007 and Pmscar053 produced more bands,which was 13.1%,13.3%,18.4% of the total bands respectively,while the primers Pmscar039 and Pmscar499 produced less band,and they were 0.16% and... |
| Keywords: | MuPS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《南京林业大学学报(自然科学版)》浏览原始摘要信息 | |
| 点击此处可从《南京林业大学学报(自然科学版)》下载免费的PDF全文 | |