| 大鼠药物依赖性便秘模型的制作 |
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| 引用本文： | 巫全胜,吴曙光,赵菊花,郭科男,董小君,钱宁. 大鼠药物依赖性便秘模型的制作[J]. 实验动物科学, 2007, 24(4): 70-72 |
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| 作者姓名： | 巫全胜 吴曙光 赵菊花 郭科男 董小君 钱宁 |
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| 作者单位： | 1. 江西宜春职业技术学院医学基础部
2. 贵阳中医学院动物实验研究所,贵阳,550002 |
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| 摘 要： | 目的建立一种大鼠药物依赖性便秘的动物模型。方法不禁食,限水,致大鼠形成便秘;然后用大黄,酚酞灌胃给予治疗,起始剂量每天20 mg/100 g/d,按每天20 mg/100 g/d递增;大鼠对药物产生依赖后,再逐渐停止限水,大黄最终用量320 mg/100 g/d,酚酞最终用量420 mg/100 g/d,再维持此剂量不变饲养三个月。观察实验动物大鼠肠道的传输功能:粪便排出量,粪便的干重,首粒大便排出时间,大便内酚红含量,大便中红色塑料小球排出情况,小肠推动率。结果与正常对照组比较,各模型组小肠推动率减慢,首粒大便排出时间长,排便量少,大便内酚红含量减少。结论成功地建立了一种大鼠药物依赖性便秘的动物模型,该模型简单经济,可重复性强。
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| 关 键 词： | 大鼠 药物依赖性便秘 动物模型 |
| 文章编号： | 1006-6179（2007）04-0070-03 |
| 收稿时间： | 2006-11-30 |
| 修稿时间： | 2006-11-30 |
Development of Drug-Dependent Constipation in Rats |
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| WU Quan-sheng,WU Shu-guang,ZHAO Ju-hua,GUO Ke-nan,DONG Xiao-jun,QIAN Ning. Development of Drug-Dependent Constipation in Rats[J]. Laboratory Animal Science, 2007, 24(4): 70-72 |
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| Authors: | WU Quan-sheng WU Shu-guang ZHAO Ju-hua GUO Ke-nan DONG Xiao-jun QIAN Ning |
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| Affiliation: | Institute of Laboratory Animal, Guiyang College of Traditional Chinese Medicine, Guiyang 550002 |
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| Abstract: | Objective To develop the model of drug-dependent constipation in rats. Methods The constipation was induced in the rats by ad lib food and limited water-intake. Then the rats with constipation were treated by oral administration of phenothalin solution and rhubarb soak at the initial dose of 20 mg/100 g/d. The dose was increased daily by 20 rag/100 g, with the final dose of 420 rag/100 g/d and 320 rag/100 g/d, respectively. The drugs were continuously given at the final dose for three months to induce drug-dependent constipation. The intestinal transit functions of the rats, such as small intestine advance rate, stools quantity and dry weight, time of first having stools, phenol red content in stools, and number of red small plastic ball in stools, were observed in both normal and model groups. Results As compared with the rats of the normal group, the small intestine advance rate was slow, the time of first having stools was laggard, and stool quantity and phenol red content in stools were decreased in the rats of the model group. Conclusion The model of drug-dependent constipation was successfully developed in the rat, which was simple, economical, and reproducible. |
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| Keywords: | Rat Drug-dependent constipation Animal model |
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