| 海洋深层水与褐藻糖胶配伍应用抑制HepG2肝细胞脂质积聚调节作用的研究 |
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| 引用本文： | 海洋深层水与褐藻糖胶配伍应用抑制HepG肝细胞脂质积聚调节作用的研究. 海洋深层水与褐藻糖胶配伍应用抑制HepG2肝细胞脂质积聚调节作用的研究[J]. 山东科学, 2015, 28(6): 11-16. DOI: 10.3976/j.issn.1002-4026.2015.06.003 |
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| 作者姓名： | 海洋深层水与褐藻糖胶配伍应用抑制HepG肝细胞脂质积聚调节作用的研究 |
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| 作者单位： | 1. 山东中医药大学药学院,山东 济南 250399;2. 山东省科学院生物研究所,山东省生物传感器重点实验室,山东 济南 250014;3. 海洋药物教育部重点实验室,中国海洋大学医药学院,山东 青岛 266003 |
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| 基金项目： | 山东中医药大学“名科工程”青年骨干培养计划（ZYDXY1323） |
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| 摘 要： | 以Hep G2细胞为模型,研究海洋深层水(deep sea water,DSW)与褐藻糖胶(fucoidan,FPS)配伍应用对Hep G2细胞脂质代谢的调节作用。采用MTT法确定与DSW配伍应用的FPS浓度控制在50μg/m L以内。在这一范围内,随着FPS浓度的增加(分别为5,10,20,50μg/m L),Hep G2细胞胞内的脂质含量逐渐降低,呈现明显的剂量依赖关系。而且,DSW与50μg/m L的FPS配伍应用的效果显著优于单用同剂量的DSW或FPS。实验结果表明,DSW与FPS配伍应用对Hep G2细胞脂质代谢具有更好的调节作用,其最佳配伍应用浓度为50μg/m L,这为高脂血症的防治提供了一个新的思路。
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| 关 键 词： | 脂质积聚 海洋深层水 褐藻糖胶 配伍应用 HepG2细胞 |
| 收稿时间： | 2015-08-26 |
Matched application of deep sea water and fucoidin in attenuation of lipid accumulation in HepG2 cells |
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| He Shan,Peng Wei bing,Zhou Hong lei,Liu Ke chun,Xia Xuan. Matched application of deep sea water and fucoidin in attenuation of lipid accumulation in HepG2 cells[J]. Shandong Science, 2015, 28(6): 11-16. DOI: 10.3976/j.issn.1002-4026.2015.06.003 |
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| Authors: | He Shan Peng Wei bing Zhou Hong lei Liu Ke chun Xia Xuan |
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| Affiliation: | 1.School of Pharmacy, Shandong University of Traditional Chinese Medicine,Jinan 250399,China;2.Shandong Provincial KeyLaboratory of Biosensors, Biology Institute, Shandong Academy of Sciences, Jinan 250014, China;3.Ministry of EducationKey Laboratory of Marine Drugs, School of Pharmacy, Ocean University of China, Qingdao 266003, China |
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| Abstract: | We addressed regulation effect of matched application of deep sea water (DSW) and fucoidan (FPS) on lipid metabolism with HepG2 cells as models. We confirmed the concentration of FPS matched with DSW should be controlled within 50 μg/mL by MTT assay. Within this scope, lipid content of HepG2 cells gradually decreased with the increase of FPS concentration (5, 10, 20, and 50 μg/mL). Significant doze dependent phenomenon exists. Moreover, matched application of DSW and 50 μg/mL FPS was significantly superior to single 50 μg/mL DSW or FPS.Experimental results show that matched application of DSW and FPS has better regulation effect to lipid metabolism of HepG2 cells, optimal matched application concentration of 50 μg/mL. This research provides a new solution for prevention and treatment of hyperlipidemia. |
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| Keywords: | deep sea water HepG2 cells fucoidan matched application lipid accumulation |
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