| 酶法生产鸟氨酸菌株的筛选与培养 |
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| 引用本文： | 张鹏,徐焘,张骁,陈畅. 酶法生产鸟氨酸菌株的筛选与培养[J]. 北京化工大学学报(自然科学版), 2009, 36(6): 85-90 |
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| 作者姓名： | 张鹏 徐焘 张骁 陈畅 |
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| 作者单位： | 北京化工大学,生命科学与技术学院,北京,100029;北京化工大学,生命科学与技术学院,北京,100029;北京化工大学,生命科学与技术学院,北京,100029;北京化工大学,生命科学与技术学院,北京,100029 |
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| 基金项目： | 北京化工大学青年教师自然科学基金 |
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| 摘 要： | 从土壤中筛选到一株可以利用胞内精氨酸酶生产鸟氨酸的菌株XT-025，通过形态特征、生理生化特性及16S rRNA基因序列分析，初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis)，这是首次发现该种微生物可用以生产鸟氨酸。通过对其培养条件和转化条件的优化，确定了最佳的培养时间为24h，最合适的转化液成分为0.3mol/L的碳酸盐缓冲液，V(Na2CO3)∶V(NaHCO3)为8∶2。同时发现Mn2+对精氨酸酶有很强激活作用，在添加适量Mn2+的条件下，鸟氨酸的产量能够达到50g/L，精氨酸摩尔转化率达到100%，而Sn4+和Cu2+则会使酶完全失活。
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| 关 键 词： | 鸟氨酸 精氨酸 精氨酸酶 苏云金芽孢杆菌 |
| 收稿时间： | 2009-03-02 |
Screening of bacterium for the conversion of arginine into ornithine by arginase |
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| ZHANG Peng,XU Tao,ZHANG Xiao,CHEN Chang. Screening of bacterium for the conversion of arginine into ornithine by arginase[J]. Journal of Beijing University of Chemical Technology, 2009, 36(6): 85-90 |
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| Authors: | ZHANG Peng XU Tao ZHANG Xiao CHEN Chang |
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| Affiliation: | College of Life Science and Technology, Beijing University of Chemical T echnology, Beijing 100029, China |
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| Abstract: | A strain of bacteria XT-025 for producing ornithine by arginase was screened from soil. This strain was identified as Bacillus thuringiensis according to its morphology, physiological characteristics and 16S ribo-somal RNA gene sequence. This is the first report of the production of ornithine by this microbe. The optimum culture time was found to be 24 h with a cushion solution composed of 0.3mol/L carbonate and V(Na_2CO_3): V (NaHCO_3) =8 = 2. It was found that Mn~(2+) can activate arginase and by adding appropriate concentrations of Mn~(2+), the yield of ornithine can reach 50 g/L and the molar conversion rate of arginine can reach 100%. In contrast, Sn~(4+) and Cu~(2+) were found to inhibit the reaction. |
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| Keywords: | ornithine arginine arginase Bacillus thuringiensis |
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