| 基于二氰基异佛尔酮的荧光探针用于细胞中半胱氨酸的检测 |
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| 引用本文： | 王志宇,沈舒婷,杨静,胡磊,王慧. 基于二氰基异佛尔酮的荧光探针用于细胞中半胱氨酸的检测[J]. 海南师范大学学报(自然科学版), 2024, 37(2): 158-163 |
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| 作者姓名： | 王志宇 沈舒婷 杨静 胡磊 王慧 |
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| 作者单位： | 皖南医学院 药学院，安徽 芜湖 241002 |
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| 基金项目： | 安徽省自然科学基金项目（1908085QB50）；安徽省大学生创新创业教育训练计划项目（S202310368089） |
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| 摘 要： | 半胱氨酸（Cys）在生物体系中起着不可缺少的作用，可以参与人体内的许多生理和病理过程，对氧化还原稳态和细胞活性的调节有重要作用。大量研究表明，Cys浓度异常与许多疾病的发生密不可分，因此有必要开发特异性检测Cys的荧光探针。本文以丙烯酰基为识别单元，以二氰基异佛尔酮为荧光基团，设计合成了一种荧光探针ISO-EV-AC。实验结果表明，探针ISO-EV-AC对Cys的检测展现出较高的选择性，不受其他分析物的干扰，检测限低至31 nmol/L，响应Cys的时间为5 min。细胞实验结果表明探针ISO-EV-AC可用于检测细胞中的Cys。
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| 关 键 词： | 半胱氨酸 荧光探针 丙烯酰氯 二氰基异佛尔酮 |
A Fluorescent Probe Based on Isophorone Dicyanide for the Detection of Cysteine in Cells |
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| WANG Zhiyu,SHEN Shuting,YANG Jing,HU Lei,WANG Hui. A Fluorescent Probe Based on Isophorone Dicyanide for the Detection of Cysteine in Cells[J]. Journal of Hainan Normal University:Natural Science, 2024, 37(2): 158-163 |
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| Authors: | WANG Zhiyu SHEN Shuting YANG Jing HU Lei WANG Hui |
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| Affiliation: | School of Pharmacy，Wannan Medical College， Wuhu 241002， China |
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| Abstract: | Cysteine (Cys) plays an indispensable role in biological systems, which can participate in a variety of physiological and pathological processes in the human body and plays an essential role in the regulation of redox homeostasis and cell activity. A large number of studies have shown that abnormal Cys concentrations are strongly associated with the occurrence of various diseases. Therefore, it is necessary to develop a specific fluorescence probe for Cys detection. In this paper, a fluorescent probe ISO-EV-AC was designed and synthesized using acryloyl group as the recognition unit and isophorone dicyano as the fluorophore. The results indicated that probe ISO-EV-AC exhibits a high selectivity for the detection of Cys without interference from the other nanlytes. The detection limit was as low as 31 nmol/L and the response time of Cys was 5 min. The results of the cell experiments demonstrated that probe ISO-EV-AC can be used to detect Cys in cells. |
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| Keywords: | cysteine fluorescent probe acryloyl chloride isophorone dicyano |
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