| 火炬松体胚发生与几种专利培养基的应用 |
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| 引用本文： | 吴丽君,叶建仁. 火炬松体胚发生与几种专利培养基的应用[J]. 南京林业大学学报(自然科学版), 2008, 32(1): 1-5 |
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| 作者姓名： | 吴丽君 叶建仁 |
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| 作者单位： | 南京林业大学森林资源与环境学院,江苏,南京,210037;福建省林业科学研究院,福建,福州,350012;美国弗吉尼亚理工学院与州立大学,弗吉尼亚,黑堡,24061;南京林业大学森林资源与环境学院,江苏,南京,210037 |
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| 基金项目： | 美国弗吉尼亚理工学院与州立大学基金 |
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| 摘 要： | 以火炬松优良家系20692、20615、201036、20639不同球果采收期的含未成熟合子胚的雌配子体为外植体,利用4种针叶树体胚发生专利培养基AL、TX、LP、DS,分别与AL、TX、LP培养基激素组合及无激素组合形成16种诱导培养基,开展不同家系、基本培养基、球果采收期对火炬松体细胞胚胎发生率影响的研究。结果表明:合子胚发育的第2、3、4阶段为最理想外植体，不同家系、基本培养基、激素组合对ESM诱导率及外植体褐化率均存在显著影响，16种组合的培养基中,在LPLP、LPTX、ALTX培养基上可获得较高的体胚诱导率,分别为18%、16%、11%.
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| 关 键 词： | 火炬松 体细胞胚胎发生 专利培养基 诱导率 胚性胚柄细胞团 |
| 文章编号： | 1000-2006（2008）01-0001-05 |
| 收稿时间： | 2007-02-12 |
| 修稿时间： | 2007-09-19 |
A study on somatic embryogenesis in loblolly pine and application of several patent media |
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| WU Li-jun,YE Jian-ren. A study on somatic embryogenesis in loblolly pine and application of several patent media[J]. Journal of Nanjing Forestry University(Natural Sciences ), 2008, 32(1): 1-5 |
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| Authors: | WU Li-jun YE Jian-ren |
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| Affiliation: | 1.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China;2.Fujian Academy of Forestry, Fuzhou 350012,China;3.Virginia Polytechnic Institute and State University, Virginia Blacksburg 24061,USA |
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| Abstract: | Exploring somatic embryogenesis in loblolly pine is important in performing clonal propagation and developing the genetic transformation. The megagametophyte,i, e. , immature zygotic embryos from the different cone collection time of the elite families 20 -692,20 -615,20 - 1036 and 20 -639 was used as explants. The four patent media AL, TX, LP and DS for somatic embryogenesis were combined with different hormones and no hormone to form 16 initiation media. The effects of different patent media, families and Cone collection time on induction rate of somatic embryos were studied. The results showed that the best explants were immature zygotic embryos of development stage 2, 3, 4. There was significant difference in ESM induction rates, explants browning using different families, basic media and hormone combinations. Among the 16 combination media tested, the media LP - LP,PL - TX and AL - TX got higher induction rate,i.e. ,18%,16% and 11%, respectively. |
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| Keywords: | Pinus taeda L. Somatic embryogenesis Patent media Initiation rate Embryogenic suspensor mass |
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