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| 亚硝基胍与紫外诱变选育高效解钾菌株 | |
| 引用本文： | 彭钰媛,易浪波,彭清忠,彭清静. 亚硝基胍与紫外诱变选育高效解钾菌株[J]. 吉首大学学报(自然科学版), 2016, 37(6): 62-67. DOI: 10.3969/j.cnki.jdxb.2016.06.013 |
| 作者姓名： | 彭钰媛 易浪波 彭清忠 彭清静 |
| 作者单位： | （吉首大学生物资源与环境科学学院，湖南 吉首 416000） |
| 基金项目： | 国家自然科学基金资助项目(21266008)；湖南省研究生培养创新基地项目（JDST201503）；湘西州科技计划项目 |
| 摘 要： | 为构建遗传背景差异较大、解钾性能稳定提高的候选菌库，选用课题组分离的蜡样芽孢杆菌L18（Bacillus cereus，KF494191) 为出发菌株，对其分别进行亚硝基胍（NTG）和紫外（UV）诱变处理，经四苯硼钠-季铵盐显色初筛，摇瓶发酵复筛，获得42株解钾能力显著增强的突变株，其中：诱变株L18-NTG-151和L18-UV-68发酵液钾质量浓度分别为126.74，121.69 mg/L，与出发菌株相比分别提高了63.64%和57.12%.结果表明亚硝基胍和紫外线诱变处理能显著提高蜡样芽胞杆菌的解钾能力. |
| 关 键 词： | 亚硝基胍 紫外 诱变育种 解钾菌株 |
Screening of High Efficiency Potash Feldspar-Solubilizing Bacteria by NTG/UV |
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| PENG Yuyuan,YI Langbo,PENG Qingzhong,PENG Qingjing. Screening of High Efficiency Potash Feldspar-Solubilizing Bacteria by NTG/UV[J]. Journal of Jishou University(Natural Science Edition), 2016, 37(6): 62-67. DOI: 10.3969/j.cnki.jdxb.2016.06.013 | |
| Authors: | PENG Yuyuan YI Langbo PENG Qingzhong PENG Qingjing |
| Affiliation: | (College of Biology and Environmental Sciences，Jishou University，Jishou 416000，Hunan China) |
| Abstract: | In order to build a bacteria library with large difference genetic background and the Potassium-solubilizing performance，an original Silicate bacteria L18 (Bacillus cereus，KF494191) was selected.It was induced mutagenesis by nitrosoguanidine (NTG) culturing and ultraviolet (UV) radiating.42 mutant bacteria with obviousincreased potassium-solubilizing performance were screened from a large number of mutant bacteria.The K content in fermentation of 2 mutant bacteria (L18-NTG-151,L18-UV-68) were 126.74 mg/L and 121.69 mg/L，which were improved by 63.64% and 57.12% compared with original bacteria L18 (77.45 mg/L).The results show thatboth two methods of NTG or UV can significantly improve the potassium-solubilizing capacity of Bacillus cereus. |
| Keywords: | NTG UV mutation breeding Potassium-solubilizing bacteria |
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