Production of insulin-like growth factor I (IGF-I) and IGF-binding proteins by rat intestinal stromal cells in vitro |
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| Authors: | R G MacDonald R H McCusker D J Blackwood J A Vanderhoof J H Y Park |
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| Institution: | (1) Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha (Nebraska 68198-4525, USA), US;(2) Department of Animal Sciences, University of Illinois, Urbana (Illinois 61801, USA), US;(3) Department of Pediatrics, University of Nebraska Medical Center, Omaha (Nebraska 68198-2165, USA), US;(4) Department of Food Sciences and Nutrition, Hallym University, 1 Okchon Dong, Chunchon 200-702 (South Korea), Fax +82 361 56 3420, e-mail: JYOON@SUN.HALLYM.AC.KR, KR |
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| Abstract: | To determine if intestinal stromal cells secrete diffusible factors such as insulin-like growth factors (IGFs) capable of
regulating epithelial cell growth in vitro, stromal cells were isolated by enzymatic digestion of rat intestine. Incorporation
of 3H]thymidine into DNA and 14C]leucine into protein of IEC-6 cells, a model intestinal epithelial cell line, was significantly increased (two- to threefold)
when the IEC-6 cells were co-cultured with stromal cells, relative to IEC-6 cells grown alone. Medium conditioned by stromal
cells stimulated DNA synthesis of IEC-6 cells in a dose-dependent manner. Analysis of the conditioned medium revealed that
intestinal stromal cells secreted IGF-I, but little IGF-II, in addition to an M
r 32,000 IGF-binding protein (IGFBP-2) and an IGFBP having M
r∼ 24,000. We conclude that rat intestinal stromal cells secrete one or more diffusible factors, which may include IGF-I and
IGFBPs, capable of stimulating proliferation of IEC-6 cells in vitro.
Received 25 August 1997; received after revision 7 November 1997; accepted 20 November 1997 |
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| Keywords: | , Insulin-like growth factors, IGFs, IGFBP, stroma, epithelium, stromal-epithelial interactions, |
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