Fehlermöglichkeiten bei der Bestimmung von Metabolitgehalten in Hefezellen

Summary The exact assay of metabolites like NAD, ATP and other glycolytic substances in intact yeast cells greatly depends on the method of cell extraction. Reproducible and high levels can be obtained after prolonged incubation at high temperatures or by freezing and thawing of the denatureted cellular material in perchloric acid, thus eliminating possible errors due to different recoveries in the cell-free extracts.