Production of antibodies to amanitins as the basis for their radioimmunoassay |
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Authors: | L Fiume C Busi G Campadelli-Fiume C Franceschi |
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Institution: | (1) Istituto di Patologia generale and Istituto di Microbiologia e Virologia, University of Bologna, Via San Giacomo 14 and 12, I-40126 Bologna, (Italy) |
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Abstract: | Summary The production of antibodies against amanitins is described. By means of these antibodies, a radioimmunoassay was developed
which allows detection of as little as 0.5 ng of amanitins in 1 ml of serum. By this method, the clearance of α-amanitin from
the blood of poisoned mice was measured.
Acknowledgments. We thank ProfessorT. Wieland and Dr.H. Faulstich (Heidelberg) for their generous gift of β-amanitin and 3H]O-methyl-demethyl-γ-amanitin. This work was supported by grants from C.N.R., Rome. |
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