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| 冷冻干燥法保存人骨髓间充质干细胞的实验初探 | |
| 引用本文： | 范菊莉,张绍志,孙洁,陈光明.冷冻干燥法保存人骨髓间充质干细胞的实验初探[J].世界科技研究与发展,2012,34(1):134-136. |
| 作者姓名： | 范菊莉 张绍志 孙洁 陈光明 |
| 作者单位： | 1. 南京航空航天大学航空宇航学院,南京210016;浙江大学制冷与低温研究所,杭州310027 2. 浙江大学制冷与低温研究所,杭州,310027 3. 浙江大学医学院,杭州,310016 |
| 基金项目： | 国家自然科学基金青年基金，中科院“低温工程重点实验室”开放基金，中国博士后科学基金 |
| 摘 要： | 尝试用冷冻干燥法来保存骨髓间充质干细胞.通过差示扫描量热仪(DSC)测量样品玻璃化转变温度,从而科学确定冻干工艺温度,合理设计冷冻干燥工艺,实验实现了以30％ PVP、20％海藻糖为保护液的骨髓间充质干细胞的冻干保存,其复水率达(55.2±7.66)％.该研究对解决现有的hBM-MSCs的保存瓶颈具有一定的参考价值. |
| 关 键 词： | 人骨髓间充质干细胞 冷冻干燥保存 DSC |
Experimental Study on Lyophilization Preservation of Human Bone Marrow Mesenchymal Stem Cells |
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| FAN Juli , ZHANG Shaozhi , SUN Jie , CHEN Guangming.Experimental Study on Lyophilization Preservation of Human Bone Marrow Mesenchymal Stem Cells[J].World Sci-tech R & D,2012,34(1):134-136. | |
| Authors: | FAN Juli ZHANG Shaozhi SUN Jie CHEN Guangming |
| Institution: | 1. College of Aerospace Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016; 2. Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027 ; 3. College of Medieal,Zhejiang University,Hangzhou 310016) |
| Abstract: | The lyophilization preservation of hBM-MSCs was conducted . 30% PVP and 20% Trehalose were used as freeze-drying protectants. The vitrification temperatures of samples were measured by DSC in order to define the freeze-drying temperature scientifically. After lyophilization, hBM-MSCs were successfully resuscitated,the recovery rate reached at （55.2 ± 7.66 ）%. This work will make certain references to the researches of hBM-MSCs preservation. |
| Keywords: | hBM-MSCs lyophilization preservation DSC |
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