| 莫氏兰根总黄酮提取工艺优选及体外抗氧化活性研究 |
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| 引用本文： | 董力玮,;翟富荣,;纪明慧,;陈光英,;孙崇格,;黄筱娟,;王友志.莫氏兰根总黄酮提取工艺优选及体外抗氧化活性研究[J].海南师范大学学报(自然科学版),2014(1):42-48. |
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| 作者姓名： | 董力玮 ;翟富荣 ;纪明慧 ;陈光英 ;孙崇格 ;黄筱娟 ;王友志 |
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| 作者单位： | [1]海南师范大学省部共建热带药用植物化学教育部重点实验室,海南海口571158; [2]三亚柏盈热带兰花产业有限公司,海南三亚572000 |
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| 基金项目： | 海南省重大科研项目（ZDZX20100007）;海南省应用技术研究与开发项目（ZDXM20130020） |
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| 摘 要： | 研究莫氏兰根中总黄酮的提取工艺及提取物抗氧化活性.以总黄酮得率为评价指标,以正交实验优选总黄酮提取的最佳工艺;考察莫氏兰根微波提取物的抗氧化活性.实验结果表明,莫氏兰根中总黄酮最佳提取工艺为:φ(EtOH)=75%、m(莫氏兰根)∶V(EtOH)=1∶50、温度60℃、时间40 min、微波功率700 W,总黄酮得率可达1.99%.其提取物对DPPH自由基和羟基自由基的抑制浓度(IC50)分别为58.2μg/mL和181.4μg/mL.
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| 关 键 词： | 莫氏兰根 总黄酮 抗氧化 |
Study on Extraction Process of Total Flavonoids and Antioxidant Activities of Extracts ofMokara Roots |
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| Institution: | DONG Liwei, ZHAI Furong＇, JI Minghui＇＇, CHEN Guangying＇, SUN Chongge2, HUANG Xiaojuan, WANG Youzhi＇ ( 1. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, China; 2. Best Goldlines Tropical Orchid Industrial (Sanya) Co., Ltd, Sanya 572000, China) |
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| Abstract: | The extraction process of total flavonoids and antioxidant activities of extracts of Mokara roots were studied. By using the total yield of flavonoids as an evaluation standard, the optimum extraction conditions about total flavonoids were analyzed by orthogonal experiment; The antioxidant activities of the extraction of Mokara roots were studied by Mi- crowave technology. The optimum extraction conditions of total flavonoids in Mokara roots was as follows： φ （EtOH）= 75%,m（Mokararoots） ： V（EtOH）=1：50, 60℃, 40min, 700W, the yield of flavonoids up to 1.99%. The median inhibitory concentration （ICs0） of scavenging hydroxyl radical and DPPH radical were 58.2μg/mL and ! 81.4μg/mL, respectively. |
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| Keywords: | Mokara roots total flavonoids antioxidant activities |
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