| 荧光猝灭法研究溶菌酶与白藜芦醇苷的相互作用 |
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| 引用本文： | 谭平,张友玉,文艳清,张雨琴,姚守拙.荧光猝灭法研究溶菌酶与白藜芦醇苷的相互作用[J].湖南师范大学自然科学学报,2009,32(1). |
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| 作者姓名： | 谭平 张友玉 文艳清 张雨琴 姚守拙 |
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| 作者单位： | 湖南师范大学化学化工学院,化学生物学及中药分析教育部重点实验室,中国,长沙,410081 |
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| 基金项目： | 国家自然科学基金，教育部重点科研项目，湖南省教育厅、科技厅重点项目 |
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| 摘 要： | 用稳态荧光光谱法研究了溶菌酶与白藜芦醇苷的相互作用,白藜芦醇苷能显著猝灭溶菌酶的内源荧光并以静态猝灭为主;在298 K和308 K的温度下其结合常数,结合位点相近.同步荧光光谱表明白藜芦醇苷对溶菌酶的构像有影响.根据热力学参数判断溶菌酶与白藜芦醇苷的作用力以疏水力和氢键为主.白藜芦醇苷与溶菌酶的能量转移效率与结合距离分别为0.062 0和3.71 nm,其作用机制为非辐射能量转移机制.
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| 关 键 词： | 荧光光谱法 溶菌酶 白藜芦醇苷 静态猝灭 能量转移 |
Studies on the Interaction of Lysozyme with Polydatin by Fluorescence Quenching Method |
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| TAN Ping,ZHANG You-yu,WEN Yan-qing,ZHANG Yu-qin,YAO Shou-zhuo.Studies on the Interaction of Lysozyme with Polydatin by Fluorescence Quenching Method[J].Journal of Natural Science of Hunan Normal University,2009,32(1). |
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| Authors: | TAN Ping ZHANG You-yu WEN Yan-qing ZHANG Yu-qin YAO Shou-zhuo |
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| Institution: | College of Chemistry and Chemical Engineering;Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research;Ministry of Education;Hunan Normal University;Changsha 410081;China |
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| Abstract: | Binding reaction of polydatin with lysozyme is studied by steady fluorescence spectroscopy in physiological medium.Polydatin can remarkably quench the fluorescence intensity of lysozyme.It is conformed from fluorescence data that the quenching effect of polydatin on lysozyme fluorescence is a single static quenching process.The binding constants KA and binding site numbers n at 298 K and 308 K in terms of Lineweavre-Burk and double-logarithm equation respectively are also obtained,which are similar at diffe... |
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| Keywords: | polydatin lysozyme fluorescence spectroscopy static quenching non-radioactive energy transfer |
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