| 蒲公英多糖提取中脱蛋白方法的研究 |
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| 引用本文： | 杨晓杰,郑云姬,杨恩策,肖静,邵淑丽,徐兴军,陈静.蒲公英多糖提取中脱蛋白方法的研究[J].高师理科学刊,2009,29(3). |
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| 作者姓名： | 杨晓杰 郑云姬 杨恩策 肖静 邵淑丽 徐兴军 陈静 |
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| 作者单位： | 1. 齐齐哈尔大学,生命科学与工程学院,黑龙江,齐齐哈尔,161006
2. 中国科学院,微生物所,北京100101 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 研究了蒲公英多糖提取中三氯乙酸(TCA)法、酶法及TCA与木瓜蛋白酶结合脱蛋白的效果.结果表明,TCA与木瓜蛋白酶结合脱蛋白可以达到较高的脱蛋白率.蛋白脱除率可达83.18%,而糖的得率为97.59%.
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| 关 键 词： | 蒲公英 多糖 脱蛋白 酶 三氯乙酸 |
Study on the removal of protein from polysaccharides of Taraxacum ohwianum Kitag. |
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| YANG Xiao-jie,ZHENG Yun-ji,YANG En-ce,XIAO Jing,SHAO Shu-li,XU Xing-jun,CHEN Jing.Study on the removal of protein from polysaccharides of Taraxacum ohwianum Kitag.[J].Journal of Science of Teachers＇College and University,2009,29(3). |
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| Authors: | YANG Xiao-jie ZHENG Yun-ji YANG En-ce XIAO Jing SHAO Shu-li XU Xing-jun CHEN Jing |
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| Institution: | 1.School of Life Science and Engineering;Qiqihar University;Qiqihar 161006;China;2.Institute of Microbiology;Chinese Academy of Sciences;Beijing 100101 |
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| Abstract: | TCA,enzymatic hydrolysis and combination of enzyme and TCA were used to remove of protein from polysaccharides of Taraxacum ohwianum Kitag.The results was showed that the combination of papain and TCA was an effective methods which demonstrated higher percentage of removal of protein.Removal from protein rate can be as high as 83.18 %,the extracting ratio of polysaccharide is 97.59%. |
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| Keywords: | Dandelion polysaccharide removal of protein enzyme TCA |
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