| 抗癌药物紫杉醇产生菌原生质体形成及释放规律 |
| |
| 引用本文： | 周东坡,孙剑秋,于寒颖,曹立成,吴涤,王雪松,李立似,平文祥.抗癌药物紫杉醇产生菌原生质体形成及释放规律[J].高师理科学刊,2001,21(2):44-46. |
| |
| 作者姓名： | 周东坡 孙剑秋 于寒颖 曹立成 吴涤 王雪松 李立似 平文祥 |
| |
| 作者单位： | 1. 齐齐哈尔大学,生物系,黑龙江,齐齐哈尔,161006
2. 黑龙江大学,生物工程系,黑龙江,哈尔滨,150080 |
| |
| 基金项目： | 黑龙江省自然科学基金!资助项目 (93 -C -11)，黑龙江省“九五”重大攻关项目! (G98C2 0 -16-1) |
| |
| 摘 要： | 详细研究了抗癌药物紫杉醇产生菌树状多节孢原生质体形成和释放的规律 .从酶系组成、酶解时间、酶解温度、渗透压稳定剂、pH值、培养基成分、培养方式、预处理等因素对树状多节孢原生质体形成和释放的影响进行了研究 ,得出了该菌的最佳原生质体制备条件及其规律 ,为以后通过原生质体技术构建紫杉醇产生菌的工程菌株奠定了基础 .
|
| 关 键 词： | 树状多节孢 原生质体 紫杉醇 抗癌药物 |
| 文章编号： | 1007-9831(2001)02-0044-03 |
| 修稿时间： | 2001年2月15日 |
Preparation and release studies of protoplast from producing strain of anticancer drug taxol |
| |
| ZHOU Dong po ,SUN Jian qiu ,YU Han ying ,CAO Li cheng ,WU Di ,WANG Xue song ,LI Li si ,PING Wen xiang.Preparation and release studies of protoplast from producing strain of anticancer drug taxol[J].Journal of Science of Teachers＇College and University,2001,21(2):44-46. |
| |
| Authors: | ZHOU Dong po SUN Jian qiu YU Han ying CAO Li cheng WU Di WANG Xue song LI Li si PING Wen xiang |
| |
| Institution: | ZHOU Dong po 1,SUN Jian qiu 1,YU Han ying 1,CAO Li cheng 1,WU Di 1,WANG Xue song 1,LI Li si 1,PING Wen xiang 2 |
| |
| Abstract: | Studies the formation and release of protoplasts from producing strain Nodulisporium sylviforme of anticancer drug taxol,and discusses the effects of some factors on that in detail,such as enzyme systems,digesting time and temperature,osmotic pressure stabilizer,pH value,culture medium,culture method .The conclusions are drawn that the best formation conditions and release laws of the strain protoplast,which laid the foundation to construct the engineering strain producing taxol by protoplast technique. |
| |
| Keywords: | nodulisporium sylviforme protoplast taxol anticancer drug |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
