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丝裂霉素诱导人白血病K562细胞凋亡的研究
引用本文:谭祺媛,邵淑丽,陈琳,武广慧,张伟伟.丝裂霉素诱导人白血病K562细胞凋亡的研究[J].高师理科学刊,2012(3):62-66.
作者姓名:谭祺媛  邵淑丽  陈琳  武广慧  张伟伟
作者单位:齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006
基金项目:黑龙江省自然科学基金项目(C200624);黑龙江省教育厅科学技术研究项目(11511447,12511611)
摘    要:通过光学显微镜、荧光显徽镜、电子显微镜、琼腊糖凝胶电泳、流式细胞术等方法,探讨不同质量浓度丝裂霉素作用不同时间后,K562细胞凋亡的情况.结果表明,丝裂霉素能够诱导K562细胞凋亡,作用48 h的最佳质量浓度为12.5μg/mL;最适质量浓度诱导细胞凋亡的凋亡率为(28.8±1.04)%(P<0.01);丝裂霉素可影响K562细胞增殖周期中的S期,即DNA合成期,细胞在此期停滞,诱导其发生凋亡.用丝裂霉素可起到诱导K562细胞凋亡的作用,这对于指导临床用药,具有重要意义.

关 键 词:丝裂霉素  K562细胞  细胞凋亡

The research on apoptosis in human leukemic K562 cells acted by MMC
TAN Qi-yuan,SHAO Shu-li,CHEN Lin,WU Guang-hui,ZHANG Wei-wei.The research on apoptosis in human leukemic K562 cells acted by MMC[J].Journal of Science of Teachers'College and University,2012(3):62-66.
Authors:TAN Qi-yuan  SHAO Shu-li  CHEN Lin  WU Guang-hui  ZHANG Wei-wei
Institution:(School of Life Sciences and Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China)
Abstract:The multiplication and apoptosis status of K562 cells treated different time by different concentration mitomycin were observed by light microscope,fluorescence microscope,TEM,agrose gel electrophoresis of DNA and flow cytometry.The results showed that MMC could induce K562 cells apoptosis,and the best concentration was 12.5μg/mL for 48 h.The optimal concentration of apoptosis induced by apoptosis rate is(28.8±1.04)%(P<0.01).Mitomycin could affect the S phase among cellular multiplication,cell could be blocked by mitomycin and then apoptosis in this phase.Mitomycin can induce the apoptosis of human leukemic K562 cells.It is of great significance to guide clinical medication.
Keywords:Mitomycin  K562 cell  apoptosis
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