| 在碱胁迫下苜蓿愈伤组织的诱导及其特性的研究 |
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| 引用本文： | 李波,焦德志,童云飞.在碱胁迫下苜蓿愈伤组织的诱导及其特性的研究[J].高师理科学刊,2006,26(2):66-69. |
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| 作者姓名： | 李波 焦德志 童云飞 |
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| 作者单位： | 齐齐哈尔大学,生命科学与工程学院,黑龙江,齐齐哈尔,161006 |
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| 摘 要： | 以苜蓿的愈伤组织为实验材料,用NaN3诱变处理,在不同pH值的碱性环境下的苜蓿愈伤组织与对照组相比较,其脯氨酸含量、苯丙氨酸解氨酶和过氧化氢酶的酶活力3项生理指标的测定上均有所提高.结果表明:NaN3可以提高苜蓿的抗碱性,苜蓿愈伤组织中脯氨酸含量和过氧化氢酶的活力在pH9.0左右时,均达到最高后急剧下降.
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| 关 键 词： | 苜蓿 抗碱 愈伤组织 NaN_3 碱性条件 |
| 文章编号： | 1007-9831(2006)02-0066-04 |
| 修稿时间： | 2006年1月15日 |
Research on the induction of callus and its characteristic of alfalfa in alkalinity forced |
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| LI Bo,JIAO De-zhi,TONG Yun-fei.Research on the induction of callus and its characteristic of alfalfa in alkalinity forced[J].Journal of Science of Teachers＇College and University,2006,26(2):66-69. |
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| Authors: | LI Bo JIAO De-zhi TONG Yun-fei |
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| Abstract: | The callus from alfalfa and NaN3 are respectively chosen as experiment materials and mutagen.Compared to the control,proline content and enzyme activity of phenylalanineammonialyase and catalase in callus had greatly risen to some extent in the different pH alkalinity environment.It was shown that NaN3 may enhance alkali resistance of alfalfa and that proline content and catalase enzyme activity of alfalfa callus can reach the top at pH9.0 or so,then dropped suddenly. |
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| Keywords: | alfalfa alkali-resistant callus NaN3 alkalinity environment |
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