| 中药黄芩的悬浮培养及过氧化物同工酶酶谱分析 |
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| 引用本文： | 赵静,张东向,焦战战,张令昂,毕宇.中药黄芩的悬浮培养及过氧化物同工酶酶谱分析[J].高师理科学刊,2014(5):71-74. |
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| 作者姓名： | 赵静 张东向 焦战战 张令昂 毕宇 |
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| 作者单位： | 齐齐哈尔大学生命科学与农林学院; |
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| 基金项目： | 黑龙江省自然科学基金资助项目(C201013);黑龙江省教育厅科研资助项目(12521609);齐齐哈尔大学研究生创新研究项目(YJSCX2013-023X) |
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| 摘 要： | 以黄芩悬浮细胞为实验材料,研究黄芩悬浮培养过程中悬浮培养液pH值、电导率值、蔗糖质量分数,并对不同培养时间测定过氧化物同工酶酶谱进行分析,确定次生代谢产物黄芩苷与培养液及同工酶的关系.结果表明,在黄芩悬浮培养周期中,过氧化物酶酶谱变化与次生代谢产物黄芩苷的积累呈正相关,黄芩悬浮培养14 d过氧化物酶活性最强,黄芩悬浮培养第15天时,黄芩苷质量浓度最高.悬浮培养液pH值、电导率值、蔗糖质量分数随黄芩苷累积均有相应变化.
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| 关 键 词： | 悬浮培养 黄芩苷 过氧化物同工酶 |
Analysis of scutellaria root suspension culture nutrient fluid and peroxidase isoenzyme |
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| ZHAO Jing,ZHANG Dong-xiang,JIAO Zhan-zhan,ZHANG Ling-ang,BI Yu.Analysis of scutellaria root suspension culture nutrient fluid and peroxidase isoenzyme[J].Journal of Science of Teachers＇College and University,2014(5):71-74. |
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| Authors: | ZHAO Jing ZHANG Dong-xiang JIAO Zhan-zhan ZHANG Ling-ang BI Yu |
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| Institution: | ( School of Life Science and Angriculture and Forestry, Qiqihar University, Qiqihar 161006, China ) |
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| Abstract: | Scutellaria suspension cells as experimental materials, research of baicalin in suspension culture of suspension culture solution pH value, conductivity value, the concentration of sucrose, and different culture time determination of peroxide isozyme analysis, determination and cultivation of secondary metabolites of baicalin liquid and isoenzyme. In the baikal skullcap root suspension culture cycle, positive change and accumulation of secondary metabolites of baiealin spectrum peroxidase enzyme, 14 d peroxidase activity of scutellaria baicalensis georgi strongest suspension culture, suspension culture of the 15 d when the highest concentration of baicalin. Suspension culture solution pH value, conductivity value, sucrose concentration of baicalin accumulation corresponding changes. |
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| Keywords: | suspension culture baicalin peroxidase isozyme |
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