| 毛木耳多糖提取工艺的研究 |
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| 引用本文： | 王伟,陈凡,王玉玲.毛木耳多糖提取工艺的研究[J].漳州师院学报,2009(3):121-124. |
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| 作者姓名： | 王伟 陈凡 王玉玲 |
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| 作者单位： | 漳州师范学院生物科学与技术系,福建漳州363000 |
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| 基金项目： | 漳州师范学院研究基金资助项目（SK06006） |
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| 摘 要： | 本实验对毛木耳子实体粗多糖（APP）提取工艺进行了研究，实验表明：提取的最佳条件是热水浸提的料液比为1：40、pH值为6、浸提温度为70℃、浸提时间为5h，此时多糖的提取率为28．237％，Sevage法除蛋白时氯仿与正丁醇的体积比为5：1，样液与Sevage试剂的体积比为4：1时蛋白脱除率较高、多糖损失率较低，此时蛋白脱除率为74．75％，粗多糖含量为38．97％．然后经DEAE-52纤维素离子交换柱层析脱色，SephadexG-200柱层析进一步分离纯化．为毛木耳多糖的开发利用奠定了基础．
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| 关 键 词： | 提取 多糖 毛木耳 |
The Study on Extraction Technology of Polysaccharide from Auricularia Polytricha Sacc |
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| WANG Wei,CHEN Fan,WANG Yu-ling.The Study on Extraction Technology of Polysaccharide from Auricularia Polytricha Sacc[J].Journal of ZhangZhou Teachers College(Philosophy & Social Sciences),2009(3):121-124. |
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| Authors: | WANG Wei CHEN Fan WANG Yu-ling |
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| Institution: | (Department of Biological Science and Technology, Zhangzhou Normal University, Zhangzhou, Fujian 363000, China) |
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| Abstract: | Polysaccharide was isolated by hot water from Auricularia Polytricha Sacc, The extraction technology of Polysaccharide was studied in this paper. The results showed that raw material and water ratio is 1：40, the extraction pH is 6, extracting temperature is70℃, and extracting time is 5hours, the extracting rate of Polysaccharide is 2 8.2 37 %. Chloroform and n-butanol ratio is 5：1, sample solution and SEVAGE reagent ratio is 4：1, removal rate of protein is 74.75% and content of polysaccharide is 38.97%. DEAE-52 cellulose and sephadex G-200 were used in decoloring and purification of APP further, The result sets a foundation for developing and using Auricularia Polytricha Sacc. |
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| Keywords: | extraction polysaccharide Auricularia polytricha sacc |
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