测定痕量盐酸丙卡特罗新方法

基于盐酸丙卡特罗（Procaterol hydrochloride,Prh）阻抑氯酸钾（KClO3）氧化异硫氰酸荧光素（FITC）生成无磷光化合物而导致FITC的室温磷光（RTP）信号剧烈增强,以及ΔIp值与Prh含量呈线性关系的学术思想,首次建立了阻抑KClO3氧化FITC的固体基质室温磷光法（SSRTP）测定痕量Prh的新方法.该法具有很高的灵敏度（检出限为0.019 fg spot-1）,对应浓度为4.8×10-14 g ml-1）与好的选择性（相对误差为±5%时,共存离子（物）不干扰）,用于实际样品中Prh残留量的测定,结果与HPLC相吻合.同时探讨了阻抑SS-RTP测定痕量Prh的反应机理.

A New Method for Determination of Trace Procaterol Hydrochloride

A new solid substrate room temperature phosphorimetry（SSRTP） was established for the determination of trace procaterol hydrochloride（Prh）.It was based on the academic thought that Prh could inhibit the reaction of potassium chlorate oxdizing fluorescein isothiocyanate（FITC） to produce a non-phosphorescent compound,which caused the room temperature phosphorescence（RTP） signal of FITC in the system to enhance sharply.And the content of Prh was linear to the ΔIp of the system.This method was of high sensitivity（the detection limit（LD） calculated by 3Sb/k was 0.019 fg spot-1,sample volume was 0.40 μL,corresponding concentration was 4.8×10-14 g mL-1）,good selectivity（Coexistent ions or materials were of no interference when the relative error（Er） was ± 5%）.It has been applied to the determination of residual Prh in practical samples and the analysis results were in accordance with those of HPLC.The reaction mechanism of inhibiting SSRTP was also discussed.