| 地热田碱性蛋白酶菌株的选育与酶性质的研究 |
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| 引用本文： | 黄家福,潘裕添,陈俊玉,林荣榕,徐宝丹,余金富.地热田碱性蛋白酶菌株的选育与酶性质的研究[J].漳州师院学报,2011(1):48-55. |
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| 作者姓名： | 黄家福 潘裕添 陈俊玉 林荣榕 徐宝丹 余金富 |
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| 作者单位： | [1]福建漳州师范学院生物科学与技术系,福建漳州363000 [2]东山出入境检验检疫局,福建漳州363401 [3]福建福州市第四十中学,福建福州350001 [4]福建漳州科人生物技术开发有限公司,福建漳州363000 |
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| 基金项目： | 福建省自然科学基金计划项目（2010J05088） |
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| 摘 要： | 从温泉附近土样中成功分离到一株高温碱性蛋白酶产生菌株H-1,经初步鉴定为芽孢杆菌（Bacillus.）菌株H-1最佳产酶的发酵时间为67.5h,该酶在30～70℃酶活性随温度升高而增强,70℃酶活性达到最高,在pH7.0～11.0范围内较稳定,从菌株H-1液体发酵液中提取粗酶液,经CMC-I阳离子交换层析和SepHadex G-75凝胶层析,得到单一组分;通过Native-PAGE、SDS-PAGE电泳鉴定,粗酶至少含有3种碱性蛋白酶同工酶;单组分碱性蛋白酶的比活力为3875U,相对分子量约为31KDa.
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| 关 键 词： | 碱性蛋白酶 同工酶 阳离子交换层析 凝胶层析 地热田 |
Breeding of Alkaline Protease Producing Strain from Geothermal Field and Properties of the Enzyme |
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| HUANG Jia-fu,PAN Yu-tian,CHEN Jun-yu,LIN Rong-rong,XU Bao-dan,YU Jin-fu.Breeding of Alkaline Protease Producing Strain from Geothermal Field and Properties of the Enzyme[J].Journal of ZhangZhou Teachers College(Philosophy & Social Sciences),2011(1):48-55. |
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| Authors: | HUANG Jia-fu PAN Yu-tian CHEN Jun-yu LIN Rong-rong XU Bao-dan YU Jin-fu |
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| Institution: | 1.Department of Biology Science and Technology,Zhanzhou Normal University,Zhangzhou,Fujian 363000,China; 2.Dongshan Entry-Exit Inspection and Quarantine Bureau of P.R.C,Zhangzhou,Fujian 363401,China;3.The Fortieth School of Fuzhou,Fuzhou,Fujian 350001,China;4.Zhangzhou Keren Biotechnology Deveplopment Co.,Ltd.,,Zhangzhou,Fujian 363000,China) |
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| Abstract: | A thermotolerant alkaline protease producing strain,H？-1,was isolated from soil sample in the neighborhood of hot spring in Zhangzhou,Fujian province.It is primarily examined as Bacillus.The best time of producting protease was 67.5 hours after the strain H-1 was cultured.The activity of this thermotolerant alkaline protease was rising gradually from 30℃ to 70℃,reaching the climax at about 70℃,keeping stable under pH 7.0~11.0.The single component was purified from the crude culture filtrate of the strain H-1 by cation exchange chromatograpHy on CMC-I and gel filtration chromatograpHy on SepHadex G-75.The result of Native-PAGE and SDS-PAGE showed that at least three kinds of isoenzymes existed in the crude protease,the rate activity of this single component was 3875U,and its molecular weight was about 31KDa. |
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| Keywords: | Alkaline Protease Isoenzymes cation exchange chromatograpHy congeal glue layer geothermal field |
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