转基因大麦茎秆木质素含量及相关特性

以不同转基因大麦(T1,T2)及非转基因对照(WT,NT1)为供试对象,采用比色法及组织化学定位的显微分析并结合傅里叶红外光谱分析(FT-IR),研究了其茎秆木质素含量、代谢关键酶活性、茎秆解剖结构及FT-IR特征.结果表明:不同基因型大麦茎秆木质素含量、薄壁组织及维管束的数目、细胞壁中愈创木基木质素(G)、紫丁香基木质素(S)及A(S)/A(G)(吸收峰面积比)均未见显著改变.与野生型大麦比较,转基因大麦T1的木质素代谢关键酶苯丙氨酸解氨酶(PAL)活性的显著下调(降幅达66%)、过氧化物酶(POD)活性的显著上调(增幅达76%)、茎秆厚壁组织明显变薄及对-羟基苯基木质素(H)比例的显著减小...

Lignin Content and Related Characteristics of Transgenic Barley Stems

Taking different transgenic barley as test materials for pot experiment,the lignin content,key enzyme activity,stem structure and Fourier transform infrared spectroscopy of different barley stems were studied to reveal the unintended changes related to lignin metabolism in transgenic barley.The results showed that the lignin content,parenchymal tissue,numbers of vascular bundles,guaiacyl lignin(G),syringyl lignin(S) and A(S)/A(G) of transgenic barley(T1,T2) stems had no significant changes in comparison with that of the wild type(WT) and non-transgenic barley(NT1).The unintended changes of the transgenic barley T1,such as the key enzyme activities of lignin metabolism(for PAL decreased by 66% and for POD increased significantly by 76%),the sclerenchymal tissue and the ρ-hydroxyphenyl lignin(H) ratio(decreased by 59%),may be mainly caused by somaclonal variation generated from tissue culture during genetic transformation.