| 乳酸杆菌产胞外多糖培养条件的优化 |
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| 引用本文： | 宋昊芮,李彦,王乐,姚一帆.乳酸杆菌产胞外多糖培养条件的优化[J].吉首大学学报(自然科学版),2019,40(5):59. |
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| 作者姓名： | 宋昊芮 李彦 王乐 姚一帆 |
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| 作者单位： | 长沙理工大学化学与食品工程学院,湖南长沙,410114;长沙理工大学化学与食品工程学院,湖南长沙,410114;长沙理工大学化学与食品工程学院,湖南长沙,410114;长沙理工大学化学与食品工程学院,湖南长沙,410114 |
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| 基金项目： | 湖南省教育厅一般项目（18C0233）；湖南省教育厅大学生研究性学习和创新性实验计划项目（S201910536030） |
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| 摘 要： | 从自制泡菜汁中提取乳杆菌，经生化鉴定确认其细菌菌属，再从乳酸杆菌中提取EPS并用苯酚-硫酸法进行多糖含量的测定。在温度、pH值、葡萄糖含量和实验时间单因素实验的基础上，采用四因素三水平正交试验对LAB EPS产量的影响进行了研究，最终确定泡菜中乳酸杆菌获得高产胞外多糖的最佳条件和最佳组合为，温度35 ℃，葡萄糖质量分数1.5%，pH值6.5，发酵时间10 h.
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| 关 键 词： | 乳酸杆菌 分离鉴定 优化 胞外多糖 |
Optimum Cultivation Conditions for Extracellular Polysaccharide in Lactobacillus |
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| SONG Haorui,LI Yan,WANG Le,YAO Yifan.Optimum Cultivation Conditions for Extracellular Polysaccharide in Lactobacillus[J].Journal of Jishou University(Natural Science Edition),2019,40(5):59. |
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| Authors: | SONG Haorui LI Yan WANG Le YAO Yifan |
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| Institution: | (College of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, China) |
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| Abstract: | The lactobacillus extracted from homemade Sauerkraut juice was firstly biochemically identified for its bacterial species,, and then EPS extracted from the lactobacillus was determined by phenol-sulfuric acid method for its polysaccharide content. Based on single factor test, effects of temperature, pH value, glucose content, and experimental time on LAB EPS yield were studied by the orthogonal test with four factors at three different levels. The optimum conditions for high yield of extracellular polysaccharide in lactobacillus are as follows: 35 ℃, 1.5% mass fraction for glucose, pH 6.5, and 10 h fermentation. |
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| Keywords: | lactobacillus isolation and identification optimization extracellular polysaccharide |
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