| 磁场对大肠杆菌及胞内谷氨酸脱羧酶的影响 |
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| 引用本文： | 贺华君,朱元保.磁场对大肠杆菌及胞内谷氨酸脱羧酶的影响[J].吉首大学学报(自然科学版),1999,20(3):25-29. |
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| 作者姓名： | 贺华君 朱元保 |
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| 作者单位： | [1]华东理工大学生物化工学院,上海 [2]湖南大学化学化工学院,湖南长沙 |
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| 摘 要： | 研制了大肠杆菌电极 ,采用部分干燥法处理大肠杆菌膜 ,使电极寿命达到了 38d 用此电极研究分别磁化底物及大肠杆菌膜对其胞内谷氨酸脱羧酶 (GAD)活性的影响 ,发现在不同磁场强度、不同作用时间下 ,磁化底物其影响不一致 ,活性最高可达 4 5 2 % ,且有滞后和恢复效应 ;磁物菌体膜未见膜活性显著变化 磁场处理菌液 ,未见明显灭菌作用 ,也未见对其生长有显著影响
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| 关 键 词： | 磁场 微生物电极 大肠杆菌 谷氨酸脱羧酶 |
The Effect of Magnetic Field on Escherichia Coli and Glutamic Acid Decarbolxylase |
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| HE Hua-jun ,ZHU Yuan-bao ,FAN Qiu-ling ,CAO Zhi-qiang ,HE Shuang-e.The Effect of Magnetic Field on Escherichia Coli and Glutamic Acid Decarbolxylase[J].Journal of Jishou University(Natural Science Edition),1999,20(3):25-29. |
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| Authors: | HE Hua-jun ZHU Yuan-bao FAN Qiu-ling CAO Zhi-qiang HE Shuang-e |
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| Institution: | HE Hua-jun 1,ZHU Yuan-bao 2,FAN Qiu-ling 2,CAO Zhi-qiang 2,HE Shuang-e 2 |
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| Abstract: | A promising microbiology Escherichia coli-based electrode was prepared,Its life time lasts over 38 days.when the substrate was treated by magnetic field,the maximum change on the activity of Glutamic Acid Decarboxylase (GAD) reached 45.2%.The delaying and reversible effect were observed.However,when magnetic field was imposed on E.coli membrane,there is no significant change on the activity.Moreover,no magnetic field effect on the growth and synthesis of GAD by E.coli was obtained. |
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| Keywords: | magnetic field microbiology electrode escherichia coli glutamic acid decarboxylase |
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