溶藻弧菌HY9901鞭毛蛋白flaC基因的克隆和序列分析及真核表达质粒的构建

参照GenBank上登录的副溶血弧菌鞭毛蛋白flaC基因序列设计引物，PCR扩增溶藻弧菌HY9901株的flaC全长基因，序列分析结果显示该基因为1 155 bp，编码384个氨基酸.与GenBank中其他弧菌的同源基因序列比对显示，溶藻弧菌flaC基因与副溶血弧菌flaC基因的同源性最高(87%).将该基因定向克隆到真核表达质粒pcDNA3.1(+)中，获得带溶藻弧菌鞭毛蛋白flaC基因的真核表达重组质粒pcDNA-flaC，为其DNA疫苗的进一步研究奠定了基础.

Cloning and Sequencing of flaC Gene of Vibrio Alginolyticus Strain HY9901 and Construction of Its Eukaryotic Expression Recombinant Plasmid

To investigate the possibility of flaC as a candidate antigen for vaccine production,primers were designed based on flaC gene sequences published in GenBank.The flaC gene of Vibrio alginolyticus HY9901 was amplified by PCR and cloned into pMD19-T vector.Sequence analysis revealed that flaC gene is 1 155 bp and encodes a putative protein of 384 amino acids.The flaC gene sequence of V.algonilyticus showed highest identity to V.parahaemolytus(87%).The PCR product was cloned into eukaryotic expression vector pc...