原儿茶酸分子印迹聚合物的色谱识别性能

应用非共价法在四氢呋喃溶剂中合成了原儿茶酸印迹聚合物.通过静态平衡吸附实验和高效液相色谱分析，评价了分子印迹聚合物对模板及其类似物的键合行为.结果表明：由于分子印迹聚合物基体存在大量多空的识别位点，并且这些位点的大小和形状与模板分子十分匹配，因此，分子印迹聚合物对模板分子具有高的吸附能力和选择性.Scatchard分析表明印迹聚合物表面有2类不同性能的键合位点.流动相及其流速对分析物的保留行为有较大影响.

Analogue Recognition and Chromatographic Behaviors of Molecularly Imprinted Polymer for Protocatechuic Acid

A non-covalent imprinting approach was applied to synthesize protocatechuic acid (3,4-dihydroxybenzoicacid) imprinted polymers (MIPs) in the polar tetrahydrofuran by using methacrylic acid as functional monomer. Byequilibrium adsorption experiments and high performance liquid chromatography (HPLC), the rebinding properties ofthe MIPs to protocatechuic acid (template) and its analogues were evaluated. It was found that the MIPs exhibited highadsorption capability and selectivity for the template and two kinds of absorption sites in the polymer were detected byScatchard curve analysis. The eluents and the mobile phase flow rate had an important influence on the retention of theanalytes on the MIPs column. All of the results showed that the stronger affinity of the MIPs to the template than its an-alogues resulted from the size and shape recognition in the MIPs network based on hydrogen bonding interaction.