Trichoderma Reesei菌生物降解酶CIP 1和CIP 2的生物信息学分析

运用生物信息学方法，对已报道的Trichoderma reesei QM6a菌株生物降解酶CIP 1和CIP 2基因进行了生物信息学分析,以明确里氏木霉生物降解酶CIP 1和CIP 2的理化性质、二级结构、信号肽、定位、跨膜结构、磷酸化位点及进化关系.结果表明：CIP 1和CIP 2均属于稳定蛋白，含有大量无规则卷曲；信号肽剪切位点分别在19~20和17~18位的氨基酸之间；两者无螺旋卷曲结构，无跨膜结构域，并定位在分泌途径信号肽（SP）上.

Bioinformatics Analysis of Trichoderma Reesei Biodegrading Enzyme CIP 1 and CIP 2

Bioinformatics analysis was performed on the CIP 1 and CIP 2 genes of biodegradation enzymes of the reported strain Trichoderma reesei QM6a through bioinformatics methods.The physical and chemical properties,secondary structure,signal peptide,localization,transmembrane structure,phosphorylation sites and evolutionary relationships of Trichoderma reesei biodegradable enzyme CIP 1 and CIP 2 were identified.The results showed that CIP 1 and CIP 2 are stable protein,containing a large number of random curls.Signal peptide cleavage sites are between amino acids 19~20,17~18,respectively.Both have no helical crimp structure,no transmembrane domain,and are located on the secretory pathway signal peptide (SP).